The Dh of nanoparticles was determined by DLS. Typically, an aqueous nanoparticle solution was measured with a Malvern Zetasizer NanoS instrument equipped with a 4 mW He-Ne 633 nm laser module at 25 °C. Measurements were carried out at a detector angle of 173° (back scattering). Data were analyzed by the Malvern DTS 6.20 software. Dh was calculated by fitting the apparent diffusion coefficient in the Stokes–Einstein equation Dh=kT/(3πηDapp), where k is the Boltzmann constant, T is the temperature and η is the viscosity of the solvent. Dh coincides with the hydrodynamic diameter when the sample is made of monodispersed spherical particles (Dapp equals the translational diffusion Dt).
Dts 6
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The DTS 6.20 software is a comprehensive data analysis solution developed by Malvern Panalytical. It provides users with advanced tools for processing and interpreting data obtained from various analytical instruments. The software's core function is to enable efficient data management, analysis, and visualization, supporting researchers and scientists in their analytical workflows.
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4 protocols using dts 6
1
Dynamic Light Scattering for Nanoparticle Size Determination
2
DNA Compound Interaction Analysis
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All measurements were carried out using a ZetasizerNano ZS from Malvern equipped with a 4 mW He-Ne laser (633 nm wavelength) at a fixed detector angle of 173° with an avalanche photodiode detector in sodium cacodylate buffer (I = 0.05 M, pH 5.0) at 25 °C in UV-transparent microcuvettes (1 cm) equipped with a stopper. Mixtures of DNA (50 μM) and compound 4 (5–100 μM) were prepared and filtered prior to measurement through 0.20 μm nylon filters. The autocorrelation functions of the backscattered light fluctuations were analysed with the DTS 6.20 software from Malvern providing the hydrodynamic diameter (Z-average), polydispersity, and size distribution (NNLS analysis).
3
Hydrodynamic Characterization of HES and HES-MTX
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Hydrodynamic parameters of HES and HES-MTX conjugate were characterized by dynamic light scattering (DLS) technique to obtain their hydrodynamic size, polydispersity information, and zeta potential. The sample solution was illuminated by a 633 nm laser, and the light intensity scattered at an angle of 173° was measured. At least six consecutive measurements were carried out for each sample. The experimental results consist of the overall average size, size distributions by intensity, and the overall polydispersity index (PDI). All samples were measured at 25°C using a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, U.K.) in a 12-μL quartz cuvette (size measurement) and folded capillary cells (zeta potential). The HES and HES-MTX conjugate concentrations were 5.5 mmol L−1 (glucose-equiv). DLS data were analysed using DTS 6.10 software (Malvern Instruments). The intensity particle size distributions were obtained by using the General Purpose algorithm included in the DTS software.
4
Hydrodynamic Size Characterization of MPs
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The hydrodynamic size of MPs was determined using Zetasizer Nano ZS (Malvern, Worcestershire, UK), with the following parameters: refractive index (1.450) and solvent viscosity (PBS, 0.9060 10−4 Pa×s).
About three-to-six consecutive measurements of each sample were taken, with an acquisition time of 30 sec per correlation function. Data were analyzed using DTS 6.10 software (Malvern Instruments, Worcestershire, UK). Particle-size distributions were obtained using the general-purpose algorithm included in the DTS software. Each sample was prepared independently 3–4 times from the same sample as flow cytometry phenotyping was performed. All functional and further statistical analyses were performed in monodispersed samples with a polydispersity index (PdI) <0.400.
About three-to-six consecutive measurements of each sample were taken, with an acquisition time of 30 sec per correlation function. Data were analyzed using DTS 6.10 software (Malvern Instruments, Worcestershire, UK). Particle-size distributions were obtained using the general-purpose algorithm included in the DTS software. Each sample was prepared independently 3–4 times from the same sample as flow cytometry phenotyping was performed. All functional and further statistical analyses were performed in monodispersed samples with a polydispersity index (PdI) <0.400.
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