Hydrogen peroxide

All experimental solutions were prepared using Milli-Q water. Fulvic acid (FA) was purchased from IHSS. Two humic acids (HAs) differing in SUVA 254 were employed: one was from Sigma-Aldrich and the other from IHSS. Sodium persulfate was obtained from Sigma-Aldrich. Sulfuric acid (98%) was the product of Kanto Chemical and was used after dilution. Hydrogen peroxide was purchased from Kanto Chemical and sodium hydroxide was purchased from Wako Pure Chemical Industries; we prepared a 0.1 mol/L solution of the latter. UV lamps radiating at 185 nm (QGL8W-31) and 254 nm (QGL8W-21) were purchased from Iwasaki Electric. Input energy are 8 W for both lamps. The raw water sample was collected from the in ow of a drinking water treatment plant located in a sub-tropical region of Japan.

Materials: Terbium acetate tetrahydrate (>99.9%), n-BuLi (in n-hexane, 1.6 M) and hydrogen peroxide were purchased from Kanto Chemical Co., Inc. Hexafluoroacetylacetone (>95.0%), triphenylphosphine oxide (>98.0%), 2,2'-bis(diphenylphosphino)biphenyl (>98.0%), 1,4dibromobenzene (>99.0%), 4,4'-dibromobiphenyl (>98.0%) and chlorodiphenylphosphine (>97.0%) were obtained from Tokyo Chemical Industry Co., Ltd. All other chemicals and solvents were reagent grade and were used without further purification.
Apparatus: 1 H NMR spectra were recorded using a JEOL JNM-ECS400 (400 MHz). 1 H NMR chemical shifts were determined by tetramethylsilane (TMS) as an internal standard. Infrared spectra were recorded using a JASCO FT/IR-4600. Elemental analyses were performed using an Exeter Analytical, Inc. CE-440 Elemental Analyzer.

All chemicals were of analytical grade and were used without further purification.
Deionized water was prepared by means of an Elix water purification system (Millipore Co. Ltd., Molsheim, France). Lignin peroxidase was purchased from Santa Cruz Biotechnology (Dallas, TX). Hydrogen peroxide was purchased from Kanto Chemical (Tokyo, Japan). Veratryl alcohol was obtained from Tokyo Kasei Kogyo (Tokyo, Japan).
The other reagents were purchased from Wako Pure Chemicals (Osaka, Japan).
A tartrate buffer solution (50 mM, pH 2.5) was prepared by dissolving an appropriate amount of tartaric acid and adjusting the pH using a 1 M sodium hydroxide solution. A tartrate buffer solution that included 50 mM SDS was employed as a background electrolyte. The background electrolyte was prepared by disolving 2.5 mmol of SDS in 50 mL of the buffer solution. The change in pH value after the addition of SDS was less than ±0.1 pH. The standard solutions of lignin peroxidase, veratryl alcohol, and veratraldehyde were prepared by being dissolved with water. A mixture containing 2 mM veratryl alcohol and 0.4 mM H2O2 was prepared with a tartrate buffer without SDS (pH 2.5) for use as the substrate solution in the in-capillary reaction.

Honokiol and magnolol were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Rat adrenal medulla PC12 cells were obtained from American Type Culture Collection (Manassas, VA, USA). 6-OHDA and RPMI-1640 medium were purchased from Sigma-Aldrich (St Louis, MO, USA). Antibiotic-Antimycotic (100 ×) liquid, horse serum and GlutaMAX TM -I Supplement were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum was purchased from Valley Biomedical (Winchester, VA, USA). The CellTiter-Glo ® Luminescent Cell Viability Assay kit was obtained from Promega Co. (Madison, WI, USA). Hydrogen peroxide was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Cell culture Petri dishes (collagen type I coated ware) and 24-well cell culture multidisc (collagen type I coated ware) were from AGC Techno Glass Co. Ltd. (Shizuoka, Japan). The Bio-Rad Protein Assay was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). All other reagents were of the highest grade commercially available.