The following stocks were used: bamΔ86 (BDSC5427), bamDf (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057), GFP-Cid (BDSC25047), GFP-D1 (BDSC50850) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moonΔ1 (VDRC313735), moonΔ28 (VDRC313738), Bam-GFP (VDRC318001), w1118 (VDRC60000) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi2, rhiKG and UASp-GFP-Rhi were gifts of William Theurkauf; delWK36, delHN56, cuffQQ37and cuffWM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before [3 (link)]. GFP-tagged Rhi, Del, Cuff and Moon are previously described transgenes constructed by inserting N-terminal GFP into genomic BACs via recombineering [2 (link),8 (link)]. XO males were generated by crossing GFP-Rhi males to C(1)RM females. To perform GFP-Rhi ChIP, bamΔ86 and GFP-Rhi were recombined.
C 1 rm
Manufactured by Bloomington Drosophila Stock Center
The C(1)RM is a laboratory instrument designed for the cryogenic storage and handling of Drosophila stocks. It provides a controlled environment for the preservation and maintenance of fly lines at ultra-low temperatures.
Automatically generated - may contain errors
Lab products found in correlation
Bamdf, by Bloomington Drosophila Stock Center (2 mentions)
Uasp shrhi, by Bloomington Drosophila Stock Center (2 mentions)
Iso 1, by Bloomington Drosophila Stock Center (2 mentions)
Gfp rhi, by Bloomington Drosophila Stock Center (2 mentions)
Gfp del, by Vienna Drosophila Resource Center (2 mentions)
Gfp cuff, by Vienna Drosophila Resource Center (2 mentions)
Bam gfp, by Vienna Drosophila Resource Center (2 mentions)
Nos gal4, by Bloomington Drosophila Stock Center (2 mentions)
Tj gal4, by Kyoto Stock Center (2 mentions)
Bamδ86, by Bloomington Drosophila Stock Center (1 mentions)
Gfp cid, by Bloomington Drosophila Stock Center (1 mentions)
Bam86, by Bloomington Drosophila Stock Center (1 mentions)
2 protocols using c 1 rm
1
Drosophila Transgenic Stocks for ChIP-seq
2
Drosophila Germline Stem Cell Genetics
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The following stocks were used: bam ∆86 (BDSC5427), bam Df (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moon ∆1 (VDRC313735), moon ∆28 (VDRC313738), Bam-GFP (VDRC318001) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi 2 , rhi KG and UASp-GFP-Rhi were gifts of William Theurkauf; del WK36 , del HN56 , cuff QQ37 and cuff WM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before (Chen et al., 2016) (link). XO males were generated by GFP-Rhi males crossed to C(1)RM females. bam ∆86 and GFP-Rhi were recombined for testis ChIP.
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