αifnγ apc cy7 xmg1.2

Manufactured by BD

αIFNγ-APC-Cy7 (XMG1.2) is a fluorescently-labeled antibody product used in flow cytometry applications. It binds to interferon-gamma (IFNγ) and is conjugated with the fluorescent dyes APC-Cy7.

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Lab products found in correlation

Protein transport inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) αcd8α percp cy5 5 clone 53 6, by BD (2 mentions) αtnfα pe cy7 mp6 xt22, by BD (2 mentions) αmip1α pe clone 39624, by R&D Systems (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Pma ionomycin cell stimulation cocktail, by Thermo Fisher Scientific (1 mentions) αcd25 pe clone pc61, by Thermo Fisher Scientific (1 mentions) αgfp alexa 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

APC-Cy7 (76 citations)

2 protocols using αifnγ apc cy7 xmg1.2

CD8+ T Cell Cytokine Profiling

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Splenocytes were stimulated for 6 hours with DMSO or GUCY2C_254–262 and Protein Transport Inhibitor Cocktail (eBioscience). Cells were stained with Live/Dead fixable Aqua Dead Cell stain kit (Invitrogen) and αCD8α-PerCP-Cy5.5 (clone 53-6.7, BD Pharmingen) and ICS staining was performed using the BD Cytofix/Cytoperm Kit (BD Biosciences) and the following antibodies: αIFNγ-APC-Cy7 (XMG1.2) and αTNFα-PE-Cy7 (MP6-XT22) from BD Biosciences, and αMIP1α-PE (clone 39624, R&D Systems). Cells were fixed in 2% PFA and analyzed on a BD LSR II flow cytometer. Analyses were performed using FlowJo software (Tree Star).

Xiang B., Baybutt T.R., Berman-Booty L., Magee M.S., Waldman S.A., Alexeev V.Y, & Snook A.E. (2017). Prime-Boost Immunization Eliminates Metastatic Colorectal Cancer by Producing High Avidity Effector CD8+ T Cells. Journal of immunology (Baltimore, Md. : 1950), 198(9), 3507-3514.

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Cytokine Expression in CAR-T Cells

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CAR-transduced T cells were stimulated for 6 h with antigen coated on tissue culture plates at 1 μg/mL in PBS overnight at 4°C, or with Cell Stimulation Cocktail (PMA/Ionomycin, eBioscience). Incubation included the Protein Transport Inhibitor Cocktail (eBioscience) when assessing intracellular cytokines. Cells were stained with Live/Dead fixable Aqua Dead Cell stain kit (Invitrogen) and subsequently stained for surface markers using the following antibodies: αCD8α-PerCP-Cy5.5 (clone 53.6-7) and αCD69-PE (clone H1.2F3) from BD Biosciences and αCD25-PE (clone PC61.5, eBioscience). Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm Kit (BD Biosciences) and staining with the following antibodies: αGFP-Alexa-488 (Invitrogen), αIFNγ-APC-Cy7 (XMG1.2) and αTNFα-PE-Cy7 (MP6-XT22) from BD Biosciences, and αMIP1α-PE (clone 39624, R&D Systems). Cells were fixed in 2% PFA and analyzed on a BD LSR II flow cytometer. Analyses were performed using FlowJo software (Tree Star).

Magee M.S., Kraft C.L., Abraham T.S., Baybutt T.R., Marszalowicz G.P., Li P., Waldman S.A, & Snook A.E. (2016). GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity. Oncoimmunology, 5(10), e1227897.

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