High sensitivity glp 1 active chemiluminescent elisa kit

Manufactured by Merck Group

Sourced in United States

The High sensitivity GLP-1 active chemiluminescent ELISA kit is a laboratory equipment product designed to detect and quantify glucagon-like peptide-1 (GLP-1) levels in biological samples. It utilizes a chemiluminescent detection method to provide high sensitivity for the measurement of active GLP-1 concentrations.

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3 protocols using high sensitivity glp 1 active chemiluminescent elisa kit

Glucose-Induced Hormonal Responses

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GIP, GLP-1 and insulin levels were measured in plasma before and 15 minutes after mice were challenged with 4 g/kg glucose. Blood glucose was measured at the tail vain with an Abbott FreeStyle Lite glucometer (Abbott Laboratories, USA). Animals were killed with CO₂ before blood from the vena cava inferior was collected in EDTA tubes (Sarstedt AG & Co, Germany) and immediately mixed with DPP-IV inhibitor (EMD Millipore Corporation, USA). Total GIP levels in the plasma were determined with a rat/mouse total GIP ELISA kit (EMD Millipore Corporation, USA). Active GLP-1 levels in the plasma were measured using a high sensitivity GLP-1 active chemiluminescent ELISA kit (EMD Millipore Corporation, USA). Plasma insulin levels were determined with an ultra-sensitive mouse insulin ELISA kit (Crystal Chem, Inc, USA).

Röder P.V., Geillinger K.E., Zietek T.S., Thorens B., Koepsell H, & Daniel H. (2014). The Role of SGLT1 and GLUT2 in Intestinal Glucose Transport and Sensing. PLoS ONE, 9(2), e89977.

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Glucose and Oil Tolerance Test

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GLP-1 and insulin levels were measured immediately before and 30-min after 2 g/kg d-glucose and 100 μL olive oil challenge to mimic a meal in mice fasted overnight (16 h). Blood samples were collected from the tail vein in EDTA-precoated tubes containing DPP-IV inhibitor (DPP4-010, Millipore). Plasma active GLP-1 levels were determined using high sensitivity GLP-1 active chemiluminescent ELISA kit (EMD Millipore Corporation). Plasma insulin levels were measured using ultrasensitive insulin ELISA kit (Alpco). Plasma β-hydroxybutyrate was measured using an automatic chemistry analyzer AU400 (Olympus Diagnostics) in blood samples collected from non-fasted mice.

Schmitt C.C., Aranias T., Viel T., Chateau D., Le Gall M., Waligora-Dupriet A.J., Melchior C., Rouxel O., Kapel N., Gourcerol G., Tavitian B., Lehuen A., Brot-Laroche E., Leturque A., Serradas P, & Grosfeld A. (2016). Intestinal invalidation of the glucose transporter GLUT2 delays tissue distribution of glucose and reveals an unexpected role in gut homeostasis. Molecular Metabolism, 6(1), 61-72.

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Glucose-Induced Hormone Secretion Dynamics

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When performing IPGTT and OGTT, sera were harvested at 0, 15, and 30 minutes after intraperitoneal injection or oral gavage of glucose, and insulin levels in these sera were determined using Ultra Sensitive Mouse Insulin ELISA Kit (Crystal Chemical) per manufacturer’s instructions. To assess glucose-induced secretion of GLP-1 and GIP, TNFR1^−/−, TNF/LT^Δ3, and their littermate controls were fasted for 16 hours, followed by oral gavage of glucose (2 g/kg body weight). Mice were then euthanized for harvesting plasma at 0, 15, and 30 minutes after oral gavage of glucose. Plasma were obtained in the presence of EDTA (10% of blood), DPP4 inhibitor (10 μl/ml blood, Millipore), and protease and phosphatase inhibitor mini table (0.00987 g/mouse, Thermo Fisher). GLP-1 (High Sensitivity GLP-1 Active Chemiluminescent ELISA Kit, EMD Millipore Corporation), and GIP (RAT/MOUSE GIP (TOTAL) ELISA KIT, EMD Millipore Corporation) levels in these plasmas were determined per manufacturer’s instructions.

Chen S., Wei W., Chen M., Qin X., Qiu L., Zhang L., Zhang Y., Cao Q, & Ying Z. (2018). TNF Signaling Impacts Glucagon-Like Peptide-1 Expression and Secretion. Journal of molecular endocrinology, 61(4), 153-161.

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