Czc25146

Trichostatin A, tubacin and nocodazole were from Sigma Aldrich, PTL from Eurodiagnostico, LRRK2-IN1, TAE684 and CZC25146 from the Michael J. Fox Foundation, GSK2578215A from Tocris, compound 68 (ID 9108605) and compound 70 (ID 9119202) from Chembridge Corporation (San Diego, USA), and GNE-0877 and GNE-7915 from MedchemExpress (USA), and MLi2 from MRC PPU, Dundee, UK. Natural streptolysin-O was from Abcam (ab63978), and non-hydrolyzable GTP test kit from Jena Bioscience (NK-102).

EZ spheres were differentiated into dopamine neurons using FGF-8, purmorphamine, and growth factors as previously described (Ebert et al., 2013 ). Induction of sensory neurons was accomplished using an established protocol (Chambers et al., 2012 (link)). For kinase inhibition experiments, 1 μM LRRK2-IN-1, 1 μM GSK2578215A, and 200 nM CZC25146 (kindly provided by Sabine Hilfiker but commercially available through Tocris and Sigma) was added at the 2-week time point and freshly supplemented every feeding for a total of 2 weeks. DMSO (1 μM and 200 nM) was used as the vehicle control.