Vitamin E determination was performed according to the method described in EC Regulation no. 152/2009, using a high performance liquid chromatograph and a PDA-UV detector (HPLC Finningan Surveyor Plus, Thermo-Electron Corporation, Waltham, MA) at a wavelength of 292 nm. A HyperSil BDS C18 column, with silica gel, dimensions of 250 × 4.6 mm, and a particle size of 5 μm (Thermo-Electron Corporation, Waltham, MA), was used. Chromatographic analysis was carried out under isocratic conditions at a flow rate of 1.5 mL/min and a mobile phase of 4% water, using 96% methanol.
Hplc finningan surveyor plus
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HPLC Finningan Surveyor Plus is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design, allowing for customization to meet specific laboratory needs. The Surveyor Plus system includes a pump, autosampler, and photodiode array (PDA) detector, providing reliable and accurate separation and detection of a wide range of compounds.
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3 protocols using hplc finningan surveyor plus
1
Vitamin E Determination by HPLC
2
Quantification of Vitamin E, Lutein, and Zeaxanthin
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Vitamin E determination in plants and meat was performed using a high-performance liquid chromatograph (HPLC Finningan Surveyor Plus, Thermo-Electron Corporation, Waltham, MA, USA) and a PDA-UV detector at a wavelength of 292 nm [19 ].
Lutein and zeaxanthin content were analyzed using a high-performance liquid chromatograph (Perkin Elmer 200 series, Shelton, CT, USA) with a UV detector (445 nm), and a Nucleodur C18 column (Macherey-Nagel, Dueren, Germany), as described here [19 ]. The results were expressed as mg/kg.
Lutein and zeaxanthin content were analyzed using a high-performance liquid chromatograph (Perkin Elmer 200 series, Shelton, CT, USA) with a UV detector (445 nm), and a Nucleodur C18 column (Macherey-Nagel, Dueren, Germany), as described here [19 ]. The results were expressed as mg/kg.
3
Vitamin E Quantification by HPLC
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The vitamin E concentration was determined using a high-performance liquid chromatograph (HPLC Finningan Surveyor Plus, Thermo-Electron Corporation, Waltham, MA) equipped with PDA-UV detector (292 nm). Chromatographic determination was performed under isocratic conditions according to the method described in Regulation (EC) no. 152/2009 and by Varzaru et al., (2020) . The results were expressed as µg/g of dried sample.
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