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VPS35 is a protein that functions as a component of the retromer complex, which is involved in the retrieval of transmembrane proteins from endosomes to the trans-Golgi network. The retromer complex plays a role in the recycling of proteins and the maintenance of cellular homeostasis.

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Lab products found in correlation

Bclaf1, by Santa Cruz Biotechnology (1 mentions) Hsp70, by Santa Cruz Biotechnology (1 mentions) β actin, by Proteintech (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Hsp90α, by Cell Signaling Technology (1 mentions) Tsg101, by Santa Cruz Biotechnology (1 mentions) Recombinant human il 6, by Thermo Fisher Scientific (1 mentions) Py705 stat3, by Cell Signaling Technology (1 mentions) Mouse il 6, by Thermo Fisher Scientific (1 mentions) β tubulin, by Thermo Fisher Scientific (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Vps26, by Abcam (1 mentions)

Close spelling variants of this product

Anti-VPS35 antibody (2 citations) Anti-VPS35 (2 citations)

3 protocols using vps35

1

Comprehensive Western Blot Methodology

Cited 1 time
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Western blotting was performed as described previously [24 (link)]. The primary antibodies were as followed: β-actin (66,009–1–1 g, PROTEINTECH), Bclaf1 (26,809–1-AP, Santa Cruz Biotechnology), VPS35 (sc-374,372, Santa Cruz Biotechnology), HSF1 (sc-9144, Santa Cruz Biotechnology), Hsp70 (sc-69,705, Santa Cruz Biotechnology), Hsp90α (8165 s, Cell Signaling Technology), TSG101 (sc-7964, Santa Cruz Biotechnology), CD63 (25,682–1-AP, PROTEINTECH). The secondary antibodies (Donkey anti-mouse/rabbit IRDye 680/800) were purchased from Li-COR Biosciences (Lincoln, Nebraska, USA). Blots were scanned by LI-COR Odyssey infrared imaging system and quantified with Image J software v1.8.0.
Tan W., Zhang J., Liu L., Liang M., Li J., Deng Z., Zheng Z., Deng Y., Liu C., Li Y., Xie G., Zhang J., Zou F, & Chen X. (2022). Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma. Translational Oncology, 26, 101502.
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2

Silencing GPR177, VPS35, and ATP6V0 in MSCs

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MSCs were transfected with siRNA for GPR177, VPS35, and ATP6V0 (Santa Cruz), respectively on days 1, 4, and 7 by using the same protocol above described. Silencing was detected by western blotting after the treatments. The secreted Wnt3a in the collected medium was quantified by ELISA (USCN).
Lin S.S., Ueng S.W., Niu C.C., Yuan L.J., Yang C.Y., Chen W.J., Lee M.S, & Chen J.K. (2014). Effects of hyperbaric oxygen on the osteogenic differentiation of mesenchymal stem cells. BMC Musculoskeletal Disorders, 15, 56.
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3

Antibody Validation and Cell Line Characterization

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Recombinant human IL-6, OSM, INF-β, IFN-γ, and mouse IL-6 (Peprotech); mouse monoclonal antibodies against Flag (1:1000 for immunoblots (IB), 1:200 for immunofluorescent staining (IF)) and β-actin (1:1000 for IB) (Sigma), β-tubulin (Invitrogen, 1:1000 for IB), HA (Covance, 1:1000 for IB), PCNA (F-2, sc-25280, 1:1000 for IB) and VPS35 (B-5, sc-374372, 1:1000 for IB) (Santa Cruz Biotechnology); rabbit monoclonal antibodies against pY705-STAT3 (D3A7, 1:1000 for IB, 1:100 for IF), BCL-XL (#2764, 1:1000 for IB), c-MYC (D84C12, 1:1000 for IB), and p-P65 (S536, 1:1000 for IB) (Cell Signaling Technology); rabbit polyclonal antibodies against STAT3 (C-20, sc-482, 1:1000 for IB, 1:200 for IF) and p65 (C-20, sc-372, 1:1000 for IB) (Santa Cruz Biotechnology), JAK1 (#610232, BD, 1:500 for IB), VPS26 (ab-23892, Abcam, 1:1000 for IB), and TRIM27 (#18791, IBL, 1:500 for IB) were purchased from the indicated companies. Mouse and rabbit polyclonal antibodies against gp130 were raised against N-terminal portion of human gp130 protein.
HEK293, HeLa, HT29, and RKO cells were obtained from ATCC. For all the cells used, mycoplasma contamination was checked.
Zhang H.X., Xu Z.S., Lin H., Li M., Xia T., Cui K., Wang S.Y., Li Y., Shu H.B, & Wang Y.Y. (2018). TRIM27 mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis. Nature Communications, 9, 3441.
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