Cell proliferation was measured using a Cell Proliferation Assay Kit (XTT baser) (Biological Industries Ltd., Israel). The assay was performed according to the manufacturer's protocol. Cells (3 × 105/well) were cultured in 96-well plates. Before cells reached confluence, we cultured PBMC in the presence of M-CSF (100 ng/mL) and RANKL (30 ng/mL) and rebamipide (0, 1, and 3 mM). After 72 hours, the cells were collected, and cell proliferation was measured using the kit.
Cell proliferation assay kit xtt baser
Manufactured by Sartorius
Sourced in Israel
The Cell Proliferation Assay Kit (XTT-based) is a lab equipment product used to measure cell proliferation and viability. It utilizes the XTT reagent, which is reduced by metabolically active cells, resulting in the formation of a colored formazan dye that can be quantified using a spectrophotometer. This allows for the assessment of cellular metabolic activity, which is directly correlated with the number of viable cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cell proliferation assay kit xtt baser
1
Measuring Osteoclast Proliferation with Rebamipide
2
Peptide A Modulates FLS Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proliferation of FLS was measured using a Cell Proliferation Assay Kit (XTT baser) (Biological Industries Ltd. Israel). The assay was performed according to the manufacturer's protocol. Cells (0.6-1.0 × 103/well) were cultured in 96-well plates. Before cells were confluent, various concentrations of Peptide A or scrambled peptide as a control were added to the wells. After 24 or 48 h, the cells were collected, and cell proliferation was measured using the kit. Experiments were repeated 5 times. All experiments were performed in quadruplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!