ROS production was assessed using the DCFH-DA probe (S0033; Beyotime). Neurons were treated with pre-formulated DCFH-DA in the dark for 20 min at 37° C. Subsequently, the medium was removed, and neurons were rinsed thrice with a serum-free culture medium. The optical density (OD) values were either measured or captured using a fluorescence microscope. The content of 8-OHdG and MDA, as well as the activity of SOD, were determined using assay kits from Nanjing Jiancheng (Nanjing, China).
Dcfh da probe s0033
Manufactured by Beyotime
Sourced in China
The DCFH-DA probe (S0033) is a fluorescent dye used for the detection of reactive oxygen species (ROS) in cells. It is a non-fluorescent compound that becomes fluorescent upon oxidation, providing a way to measure the levels of ROS in a sample.
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Lab products found in correlation
2 protocols using dcfh da probe s0033
1
ROS Detection in Neurons
2
Measuring Cellular ROS Levels in Human Leukemia Cells
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Human leukemia cells in 24-well plates were incubated at 37 °C for 30 min with 500 μl of 10 μmol/L DCFH-DA probe (S0033; Beyotime Institute of Biotechnology, Haimen, China), with shaking every 5 min. The cells were then washed with PBS (three times, 5 min each) to remove any remaining extracellular DCFH-DA probe [23 (link)]. The fluorescence intensity, representing cellular ROS levels, was detected using a Gemini XPS fluorimetric microplate reader (MolecularDevices, Shanghai, China), with excitation and emission wavelengths of 488 and 525 nm, respectively.
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