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Orca flash 4.0 mounted

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The Orca Flash 4.0 is a scientific-grade sCMOS camera designed for high-speed, high-resolution imaging applications. It features a large sensor size, low read noise, and high quantum efficiency, making it suitable for a wide range of scientific research and industrial applications.

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Lab products found in correlation

Shandon cytospin 4, by Thermo Fisher Scientific (2 mentions) Alexafluor488 conjugated ab specific to relmα, by Bioss Antibodies (2 mentions) Las advanced fluorescence software, by Leica (2 mentions) Vectashield mounting medium, by Vector Laboratories (2 mentions)

Close spelling variants of this product

Orca Flash 4.0 (385 citations) Flash 4.0 (72 citations)

2 protocols using orca flash 4.0 mounted

1

Immunofluorescent Staining of Sorted Lung B Cells

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Sorted B cells (2 ×105) from lung tissue were suspended in 200 μL of 1X PBS with 2.5% FCS. The sorted cell suspensions were loaded into a Shandon Cytospin 4 (Thermo Electron Corporation, Waltham, MA), spun at 800–1000 rpm for 5 min and stored at −80°C. Frozen cytospin slides were thawed at room temperature for 30 min, fixed in 4% PFA for 15 min, and stained with an AlexaFluor488-conjugated Ab specific to RELMα (Bioss Inc., Woburn, MA). Coverslips were applied to the slides using Vectashield mounting medium (Vector Laboratories, Burlingame, CA) with DAPI. Images were taken using a Leica DM6000B fluorescent microscope, Orca Flash 4.0 mounted digital camera (Hamamatsu Photonics K.K., Japan) and LAS Advanced Fluorescence software (Leica Microsystems, Buffalo Grove, IL). Fluorescent channels were photographed separately and then merged. Exposure times and fluorescence intensities were normalized to appropriate control images.
Chen F., Wu W., Jin L., Millman A., Palma M., El-Naccache D.W., Lothstein K.E., Dong C., Edelblum K.L, & Gause W.C. (2018). B Cells Produce the Tissue-Protective Protein RELMα during Helminth Infection, which Inhibits IL-17 Expression and Limits Emphysema. Cell reports, 25(10), 2775-2783.e3.
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2

Immunofluorescent Staining of Sorted Lung B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted B cells (2 ×105) from lung tissue were suspended in 200 μL of 1X PBS with 2.5% FCS. The sorted cell suspensions were loaded into a Shandon Cytospin 4 (Thermo Electron Corporation, Waltham, MA), spun at 800–1000 rpm for 5 min and stored at −80°C. Frozen cytospin slides were thawed at room temperature for 30 min, fixed in 4% PFA for 15 min, and stained with an AlexaFluor488-conjugated Ab specific to RELMα (Bioss Inc., Woburn, MA). Coverslips were applied to the slides using Vectashield mounting medium (Vector Laboratories, Burlingame, CA) with DAPI. Images were taken using a Leica DM6000B fluorescent microscope, Orca Flash 4.0 mounted digital camera (Hamamatsu Photonics K.K., Japan) and LAS Advanced Fluorescence software (Leica Microsystems, Buffalo Grove, IL). Fluorescent channels were photographed separately and then merged. Exposure times and fluorescence intensities were normalized to appropriate control images.
Chen F., Wu W., Jin L., Millman A., Palma M., El-Naccache D.W., Lothstein K.E., Dong C., Edelblum K.L, & Gause W.C. (2018). B Cells Produce the Tissue-Protective Protein RELMα during Helminth Infection, which Inhibits IL-17 Expression and Limits Emphysema. Cell reports, 25(10), 2775-2783.e3.
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