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Mouse α v5 clone sv5 pk2

Manufactured by Bio-Rad

The Mouse α-V5 (clone SV5-Pk2) is a monoclonal antibody that recognizes the V5 epitope tag. The V5 epitope is a short peptide sequence derived from the P and V proteins of the paramyxovirus of simian virus 5 (SV5).

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2 protocols using mouse α v5 clone sv5 pk2

1

Antibody Panel for Microscopy Studies

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All the primers used in this study were purchased from Thermo Fisher, gene blocks were purchased from IDT DNA, and restriction enzymes were purchased from New England BioLabs. The primary antibodies used in this study are the following: mouse α-V5 (clone SV5-Pk2, Bio-Rad); rabbit α-V5 (ICL, RV5-45A-Z); rat α-hemagglutinin (HA, clone 3F10, Sigma); mouse α-tubulin (clone B-5-1-2, Sigma); rabbit α-Histone H3 (ab1971, Abcam); mouse α-Centrin (CrCen clone 20H5, EMD Millipore); and rabbit α-PfGAP45 [a gift from Julian Rayner at the University of Cambridge (54 (link))]. Secondary antibodies and other reagents (Alexa Fluor 405 NHS-Ester, SYTOX Deep Red Nucleic Acid Stain, and Hoechst 33342 Solution) used for microscopy were purchased from Thermo Fisher.
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2

Antibody Reagents for Microscopy Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the primers used in this study were purchased from Thermo Fisher, gene blocks were purchased from IDT DNA, and restriction enzymes were purchased from New England Biolabs. The primary antibodies used in this study are the following: mouse α-V5 (clone SV5-Pk2, BioRad); rabbit α-V5 (ICL, RV5-45A-Z), rat α-hemagglutinin (HA, clone 3F10, Sigma); mouse α-tubulin (clone B-5-1-2, Sigma); rabbit α-Histone H3 (ab1971, Abcam); mouse α-Centrin (CrCen clone 20H5, EMD Millipore); and rabbit α-PfGAP45 (gift from Julian Rayner at the University of Cambridge [53 (link)]). Secondary antibodies and other reagents (Alexa Fluor 405 NHS-Ester, SYTOX Deep Red Nucleic acid stain, and Hoechst 33342 solution) used for microscopy were purchased from Thermo Fisher.
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