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Pseudomonas aeruginosa p aeruginosa

Sourced in China

Pseudomonas aeruginosa (P. aeruginosa) is a species of Gram-negative bacteria that is commonly found in soil, water, and on the skin. It is a rod-shaped, aerobic bacterium that is known for its ability to thrive in a variety of environments. P. aeruginosa is often used in laboratory settings for research purposes, as it is a well-studied model organism.

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2 protocols using pseudomonas aeruginosa p aeruginosa

1

Antimicrobial Susceptibility of Common Pathogens

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The bacterial strains S. aureus (CICC 10201, China), Escherichia coli (E. coli) (CICC 10003, China), and Pseudomonas aeruginosa (P. aeruginosa) (CICC 20152, China) were purchased from the China Center of Industrial Culture Collection (CICC) and Klebsiella pneumonia (K. pneumonia) CGMCC 1.0839, Proteus vulgaris (P. vulgaris) CGMCC 1.1651, Enterobacter cloacae (E. cloacae) CGMCC 1.8726, and Acinetobacter Baumannii (A. Baumannii) CGMCC 1.10395 from the China General Microbiological Culture Collection Center. S. pyogenes is a clinical isolate found in our hospital.
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2

Bacterial Detection with Aptamer-Functionalized PEI

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Citric acid monohydrate (C6H8O7•H2O), N-hydroxy succinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Sinopharm Chemical Reagent Co. Ltd. (China). Polyethyleneimine (PEI) was purchased from Aldrich-Sigma Chemical Co. Ltd. (USA). E. coli O157:H7, Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) were purchased from China Center of Industrial Culture Collection (CICC). E. coli O157:H7 aptamer was purchased from Sangon Biotech Co. Ltd. (China) and its sequence was given as follows: 5′-COOH-GCA ATG GTA CGG TAC TTC CCC ATG AGT GTT GTG AAA TGT TGG GAC ACT AGG TGG CAT AGA GCC GCA AAA GTG CAC GCT ACT TTG CTA A-3′. All reagents were of analytical grade and used directly without any treatment. Ultrapure water (18.2 MΩ) was utilized throughout the experiment.
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