Bovine serum

Manufactured by Gemini Bio

Sourced in United States

Bovine serum is a biological material derived from the blood of cattle. It is commonly used as a supplement in cell culture media to provide essential nutrients, growth factors, and other components necessary for the growth and maintenance of cells in vitro.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (3 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Quant it picogreen assay kit, by Thermo Fisher Scientific (1 mentions) L glutamine, by Gemini Bio (1 mentions) Penicillin, by Gemini Bio (1 mentions) Streptomycin, by Gemini Bio (1 mentions) Minimum essential medium (mem), by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Flp in 293 cells, by Thermo Fisher Scientific (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Mdck siat1 cells, by Merck Group (1 mentions) Mdck siat1 cells, by Thermo Fisher Scientific (1 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions) Heat inactivated, by Gemini Bio (1 mentions) Lipofectamine, by Thermo Fisher Scientific (1 mentions) L alanyl l glutamine dipeptide, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

FBS (1100 citations) BSA (45 citations) Bovine serum albumin (BSA, (10 citations) BenchMark Fetal Bovine Serum (6 citations) Low-IgG bovine serum albumin (BSA; (4 citations) Low-endotoxin bovine serum albumin (BSA) (3 citations) Fetal bovine serum (v/v) (2 citations) Adult Bovine Serum (2 citations) Foetal bovine serum (FBS (2 citations) GemCell heat inactivated fetal bovine serum (2 citations)

6 protocols using bovine serum

Quantifying Nucleic Acid Content

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The Quant-iT ™ Picogreen ® assay kit and the Presto Blue kit were from Life Technologies (Carlsbad, US).
Bovine serum, L-glutamine, penicillin and streptomycin were from Gemini Bio-Products (Sera Laboratories International, Bolney, UK). All other reagents were purchased from Sigma-Aldrich (Poole, UK).

Viswanath A., Vanacker J., Germain L., Leprince J.G., Diogenes A., Shakesheff K.M., White L.J, & des Rieux A. (2017). Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of biomedical materials research. Part A, 105(1).

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Cell Culture Conditions for Y79-Rb and HeLa

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Cell lines were cultured as follows: Both Y79-Rb (ATCC, HTB-18; Manassas, VA) and Hela cells (ATCC, CCL-2; Manassas, VA) were grown in DMEM (Mediatech, Manassas, VA) supplemented with 5% heat-inactivated bovine serum (Gemini Bio-Products, Sacramento, CA) and 1% Penicillin/Streptomycin. Cultures were maintained at 37°C with 5% CO₂.

Liu A., Ildefonso C.J., Bond W.S., Hurwitz M.Y, & Hurwitz R.L. (2020). Inhibitors of metalloprotease, γ-sectretase, protein kinase C and Rho kinase inhibit wild-type adenoviral replication. PLoS ONE, 15(7), e0236175.

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Culturing and Embedding Stem Cells

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Previously characterised human stem cells of the apical papilla (SCAP) (RP89 cells) 27 were used between passages 6 and 9. SCAP were grown at 37°C in 5% CO2 in Minimum Essential Medium (Sigma-Aldrich) supplemented by 10% bovine serum (Gemini Bio-Products), 1% of L-glutamine (Gemini) and 1% of penicillin and streptomycin (Gemini) until they reached ~80% confluence. SCAP were then incorporated in the pre-gel solutions, placed in 96 well plates and incubated at 37°C for 1 hr.

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Influenza Virus Propagation and Cell Line Engineering

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To propagate influenza viruses, MDCK-SIAT1 cells (Millipore Sigma) were used. Cells were maintained with complete media comprising Dulbecco’s modified Eagle’s medium high glucose (DMEM; ThermoFisher) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Gemini Bio-Products), 100 units ml⁻¹ penicillin (ThermoFisher), 100 μg ml⁻¹ streptomycin (ThermoFisher), and geneticin (1 mg ml⁻¹) (ThermoFisher). To develop constitutively PB1- or HA-expressing MDCK-SIAT1 cells, one plasmid encoding both puromycin resistance and influenza genes was transfected into MDCK-SIAT1 cells using Lipofectamine 2000 (ThermoFisher). Two days post transfection, cells were transferred from 6-well plates into 10-cm dishes containing DMEM media with 10% bovine serum (Gemini Bio-Products), penicillin, streptomycin, geneticin, and puromycin (0.25 μg ml⁻¹; ThermoFisher) for selection. Medium was changed every 48 h. Clonal selection was performed using 8 or 10 mm cloning cylinders (Fisher Scientific) about 2 weeks after the transfection. Clonal cell lines were screened using a reporter virus prepared on a polyclonal cell line. To rescue influenza viruses, Flp-In 293 cells (ThermoFisher) were transfected as described below.

Creanga A., Gillespie R.A., Fisher B.E., Andrews S.F., Lederhofer J., Yap C., Hatch L., Stephens T., Tsybovsky Y., Crank M.C., Ledgerwood J.E., McDermott A.B., Mascola J.R., Graham B.S, & Kanekiyo M. (2021). A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies. Nature Communications, 12, 1722.

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Axenic Culture of E. histolytica Trophozoites

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E. histolytica strain HM1:IMSS trophozoites were grown axenically in TYI-S-33 (trypticase-yeast extract-iron-serum) medium supplemented with 1X Diamond's vitamins (SAFC Biosciences, Lenexa, KS, USA), 15% heat-inactivated bovine serum (Gemini Bio-Products, West Sacramento, CA), 100 U penicillin/ml and 100 mg streptomycin sulfate/ml (Gibco/Life Technologies, Grand Island, NY, USA), at 37°C in T-25 tissue culture flasks [31 (link)].

Linford A.S., Jiang N.M., Edwards T.E., Sherman N.E., Van Voorhis W.C., Stewart L.J., Myler P.J., Staker B.L, & Petri WA J.r. (2014). Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase. Molecular and biochemical parasitology, 193(1), 33-44.

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Establishing SH-SY5Y Neuronal Cell Lines

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Cell cultures were authenticated prior to purchase from American Type Culture Collection and again at passages 20 to 50 after purchase for morphology, growth rate, and expression of a discrete selection of human genes expressed in mature neurons (Supplement Table 1). SH-SY5Y clonal lines were grown in Dulbecco’s Modified Eagle’s Medium: Ham’s F12 (1:1) media (Gibco, Gaithersburg, MD), supplemented with 2 mM L-alanyl-L-glutamine dipeptide (GlutaMAX), 100 IU/ml penicillin, 100 µg/ml streptomycin (Thermo Fisher Scientific, Waltham, MA) and 10% heat-inactivated bovine serum (Gemini Bio Products, Sacramento, CA). Plasmids containing long isoform hCB₁ receptor, hCB₂ receptor, or the neo gene (Missouri S&T cDNA Resource Center, Rolla, MO) were transfected using Lipofectamine (Invitrogen, Life Technologies, Carlsbad, CA). The cells were selected for plasmid expression using 450 mg/L geneticin (G418) (Life Technologies, Carlsbad, CA) and clonal cell lines were isolated.

Lyons E.L., Leone-Kabler S., Kovach A.L., Thomas B.F, & Howlett A.C. (2020). Cannabinoid receptor subtype influence on neuritogenesis in human SH-SY5Y cells. Molecular and cellular neurosciences, 109, 103566.

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