Endothelial cell growth supplement pack

All chemicals including those used for immunoblotting and anti-tubulin (T5168) antibody were obtained from Sigma (Buchs, Switzerland). Antibody against p21^Cip1 (OP64) was purchased from Calbiochem (Genève, Switzerland); antibody against phosphor-p53-S15 (#9284s) was from Cell Signalling (Allschwil, Switzerland); antibodies against Arg-I (sc-18351), Arg-II (sc-20151) and p53 (sc-6243) were from Santa-Cruz (Nunningen, Switzerland); Alexa Fluor680-conjugated anti-mouse IgG (A21057); Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and dihydroethidium (DHE) were from Molecular Probes/Invitrogen (Lucerne, Switzerland); IRDye800-conjugated anti-rabbit IgG (926-32211) were from LI-COR Biosciences (Bad Homburg, Germany); the membrane-permeable 4,5-Diaminofluorescein diacetate (DAF-2DA) was from VWR international SA (Dietikon, Switzerland); X-gal was from Promega (Dübendorf, Switzerland); Endothelial cell growth supplement (ECGS) pack was from PromoCell GmbH (Allschwil, Switzerland) and all cell culture media and materials were purchased from Gibco BRL (Lucerne, Switzerland).

All chemicals, if not specifically indicated, including those used for Western blotting, mouse anti-tubulin monoclonal antibodies were obtained from Sigma (Buchs, Switzerland). Rabbit antibodies against phospho-S6-S235/236 (#2211s), S6 (#2217s) and anti-VCAM-1 (#12367S) were from Cell Signaling (Allschwil, Switzerland); mouse anti-eNOS (#610297) and anti-S6K1 (#611260) were from BD Transduction laboratories (Allschwil, Switzerland); rabbit anti-Arg-II (sc-20151) and mouse anti-ICAM-1 (sc-8439) were purchased from Santa-Cruz Biotech (Nunningen, Switzerland); Alexa Fluor680-conjugated anti-mouse IgG (A21057) and dihydroethidium (DHE) were from Molecular Probes/Invitrogen (Lucerne, Switzerland); X-gal was from Promega (Dübendorf, Switzerland); the membrane-permeable 4,5-diaminofluoresceine acetate (DAF-2DA) was from VWR international SA (Dietikon, Switzerland); QuickTiter™ Adenovirus Titer Immunoassay Kit (VPK-109, Cell Biolabs, Inc) were purchased from LuBioScience GmbH (Luzern, Switzerland); endothelial cell growth supplement (ECGS) pack was from PromoCell GmbH (Allschwil, Switzerland); all cell culture media and materials were purchased from Gibco BRL (Lucerne, Switzerland).

Reagents were purchased or obtained from the following sources: rabbit antibodies against Arg-II (sc-20151) and E-selectin (sc-14011) and mouse antibody ICAM-1 (sc-8439) were from Santa Cruz Biotech (Dallas, USA); mouse antibodies against tubulin (T5168) and β-actin (A5441) and Dimethyloxaloylglycine (DMOG) were from Sigma (St. Louis, Missouri, USA); anti-VCAM-1 (12367S) was from Cell Signaling Technology (Danvers, USA); mouse antibody against HIF1α (610958) was from BD Biosciences (New Jersey, USA); rabbit antibody against HIF2α (PAB12124) was from Abnova (Taipei, Taiwan); Alexa fluor 680 conjugated anti-mouse IgG (A21057), MitoSox, and Hoechst 33342 were from Invitrogen/Thermo Fisher Scientific (Waltham, MA USA); IRDye 800-conjugated anti-rabbit IgG (926-32211) was from LI-COR Bioconcept (Lincoln, USA); endothelial cell growth supplement (ECGS) pack was from PromoCell GmbH (Heidelberg, Germany); and all cell culture media and materials were from Gibco/Thermo Fisher Scientific (Waltham, MA USA).