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2 protocols using glacial acetic acid

1

Biomimetic Wound Dressing Fabrication

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Polycaprolactone (PCL, Mn = 80 kDa) and type A gelatin (300 g Bloom) were obtained from Sigma Aldrich (St. Louis, Missouri, USA). Chitosan (CS, 90% deacetylated powder, Mw = 200 kDa), 4-octenyl itaconate (OI), and 2-(N-morpholino) ethanesulfonic acid (MES) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Glacial acetic acid and 2,2,2-trifluoroethanol (TFE) were obtained from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Adhesive bandage was obtained from Hainuo Medical Technologies Co., Ltd. (Qingdao, China). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). N-hydroxysuccinimide (NHS) was obtained from Adamas-beta (Shanghai, China). LB Broth and LB Broth Agar were purchased from Sangon Biotech (Shanghai, China).
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2

Cisplatin-Loaded Alginate-Gelatin Hydrogel

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Cisplatin was purchased from Meryer (Shanghai) Chemical Technology Co., Ltd. CS and sodium alginate were purchased from Shanghai Macklin Inc. CaCl2 was purchased from Sun Chemical Technology (Shanghai) Co., Ltd. Gelatin was purchased from Shanghai Haohong Scientific Co., Ltd. Methacrylic anhydride, glacial acetic acid, and tetramethylenediamine were purchased from Meryer (Shanghai) Chemical Technology Co., Ltd. APS was purchased from Guangzhou Chemical Reagent Factory. Phosphate buffer and Duchenne Phosphate buffer were purchased from Beijing Solarbio Science & Technology Co., Ltd. PEGDA was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Rabbit anti-γH2AX antibody was purchased from Abcam Plc. 4′,6-Diamidino-2-phenylindole was bought from Sigma. Horseradish peroxidase-labeled goat anti-rabbit IgG antibody and Live & Dead Viability/Cytotoxicity Assay Kit was bought from Invitrogen Trading (Shanghai) Co., Ltd. Cell-CountingCCK-8 was bought from Dojindo Molecular Technologies (Japan). TUNEL apoptosis detection kit was bought from Promega (Beijing) Biotech Co., Ltd. Dulbecco's modified Eagle medium (DMEM), fetal bovine serum, and penicillin–streptomycin mix were purchased from Thermo Fisher Scientific Inc. All the other reagents and materials were used as received.
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