Anti ctbp

SKOV3 and HO8910 cells were used as representative ovarian cancer cell lines (see Supplementary materials for discussion of ovarian cancer cell lines). They were originally obtained from NICLR (National infrastructure of cell line resource) with certificates. SKOV3 and HO8910 were cultured in regular DMEM supplemented with 10% (v/v) FBS, penicillin-streptomycin (Thermo Fisher). Cisplatin, β-Estradiol (estrogen), fulvestrant, doxycycline (Dox), olaparib were all purchased from Sigma Aldrich. The anti-CtBP, anti-ERα, anti-Rad51, anti-GAPDH, anti-β-actin and anti-c-JUN antibodies were purchased from Santa Cruz (USA). The anti-caspase3, anti-PARP and the anti-γH2AX antibody was from Millipore (USA). anti-CtBP recognizes both CtBP1 and CtBP2 unless otherwise indicated. Unless specified in the manuscript, cells were cultured by normal media with physiological level of estrogen.