Normal human epidermal keratinocytes (NHEKs) from neonatal donors were obtained from ThermoFisher (Carlsbad, CA). Cells were cultured until the second passage and seeded on six-well plates for 24 h before treatments with EpiLife® media supplemented with keratinocyte growth supplement and 60 µM calcium (ThermoFisher; Carlsbad, CA).
The EpiDerm-FT™ skin cultures, reconstructed from cells of human origin, were purchased as preserved culture inserts from MatTek Corp. (Ashland, MA). They were cultured and acclimated at the air–liquid interface at 37 °C and 5% CO2 for 24 h in six-well plates. SIG-1191 formulated in water was single administered by topical application (25 µL) and incubated for 24 h.
The EpiDerm-FT™ skin cultures, reconstructed from cells of human origin, were purchased as preserved culture inserts from MatTek Corp. (Ashland, MA). They were cultured and acclimated at the air–liquid interface at 37 °C and 5% CO2 for 24 h in six-well plates. SIG-1191 formulated in water was single administered by topical application (25 µL) and incubated for 24 h.