cultured cells were imaged on an Olympus IX71 inverted microscope using Openlab
software (PerkinElmer). Chromogenic stains were imaged on a Leica DM 1000 LED.
Fiji50 (link) and/or
Photoshop (Adobe) were used to process (brightness, contrast and gamma) and
merge channels. Thick sections for 3D analysis of connectivity and clonal
analysis were imaged on a Leica upright AOBS confocal microscope and processed
and analyzed using Imaris (Bitplane) or Volocity (PerkinElmer) software. For
R26R-Confetti+/− mouse
and pSMAD3 analysis, images were acquired using a Nikon A1R GaAsP inverted SP
confocal microscope and NIS elements software and processed and analyzed using
Imaris software. Sirius-red-stained sections were imaged using a Cytation 5 cell
imaging multi-mode reader (BioTek).