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α fluoromethylhistidine

Manufactured by LGC
Sourced in United States

α-fluoromethylhistidine is a synthetic amino acid analogue used in research applications. It functions as a specific and irreversible inhibitor of the enzyme histidine decarboxylase, which is responsible for the biosynthesis of the neurotransmitter histamine.

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3 protocols using α fluoromethylhistidine

1

Pharmacological Agents Preparation and Administration

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All chemicals were used as received from the supplier. Diphenhydramine hydrochloride (20 mg kg−1 or 50 mg kg−1 Sigma Aldrich, St. Louis, MO, USA), zolantidine dimaleate (10 mg kg−1; Tocris, Minneapolis, MN, USA), immepip dihydrobromide (5 mg kg−1; Sigma Aldrich, St. Louis, MO, USA), thioperamide maleate (20 mg kg−1; Sigma Aldrich and Tocris), tacrine hydrochloride (2 mg kg−1; Tocris), and α-fluoromethylhistidine (20 mg kg−1; Toronto Research Chemicals, North York, ON, CAN) were all dissolved in sterile saline (0.9% NaCl solution, Mountainside Medical Equipment, NY, USA) at 5 mL kg−1. Reserpine (10 mg kg−1; Sigma Aldrich, St. Louis, MO, USA) was dissolved in 0.1% acetic acid (Sigma Aldrich, St. Louis, MO, USA) in sterile saline at 5 mL kg−1. Tetrabenazine (Sigma Aldrich) was dissolved in 10% DMSO (Sigma Aldrich, St. Louis, MO, USA) in sterile saline with 1 M HCl (10 µL mL−1 injection volume). All solutions were made fresh at the time of injection and all injections were given via intraperitoneal (i.p.) injection. Urethane (Sigma Aldrich, St. Louis, MO, USA) was dissolved in sterile saline as a 25% w/v solution and administered at 7 µL g−1.
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2

Pharmacological Manipulation of Histaminergic Signaling

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All chemicals were used as received from the supplier. Diphenhydramine hydrochloride (20 mg kg -1 ; Sigma Aldrich, St. Louis, MO, USA), zolantidine dimaleate (10 mg kg -1 ; Tocris, Minneapolis, MN, USA), immepip dihydrobromide (5 mg kg -1 ; Sigma Aldrich), thioperamide maleate (20 mg kg -1 or 50 mg kg -1 Sigma Aldrich and Tocris), tacrine hydrochloride (2 mg kg -1 ; Tocris), and α-fluoromethylhistidine (20 mg kg -1 ; Toronto Research Chemicals, North York, ON, CAN) were all dissolved in sterile saline (0.9% NaCl solution, Mountainside Medical Equipment, NY, USA) at 5 mL kg -1 . Reserpine (10 mg kg -1 ; Sigma Aldrich) was dissolved in 0.1 % acetic acid (Sigma Aldrich) in sterile saline at 5 mL kg -1 . Tetrabenazine (Sigma Aldrich) was dissolved in 10 % DMSO (Sigma Aldrich) in saline with 1 M HCl (10 µL mL -1 injection volume). All solutions were made fresh at the time of injection and all injections were given via intraperitoneal (i.p.) injection. Urethane (Sigma Aldrich) was dissolved in sterile saline as a 25 % w/v solution and administered at 7 µL kg -1 .
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3

Pharmacological Manipulation of Histaminergic Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were used as received from the supplier. Diphenhydramine hydrochloride (20 mg kg -1 ; Sigma Aldrich, St. Louis, MO, USA), zolantidine dimaleate (10 mg kg -1 ; Tocris, Minneapolis, MN, USA), immepip dihydrobromide (5 mg kg -1 ; Sigma Aldrich), thioperamide maleate (20 mg kg -1 or 50 mg kg -1 Sigma Aldrich and Tocris), tacrine hydrochloride (2 mg kg -1 ; Tocris), and α-fluoromethylhistidine (20 mg kg -1 ; Toronto Research Chemicals, North York, ON, CAN) were all dissolved in sterile saline (0.9% NaCl solution, Mountainside Medical Equipment, NY, USA) at 5 mL kg -1 . Reserpine (10 mg kg -1 ; Sigma Aldrich) was dissolved in 0.1 % acetic acid (Sigma Aldrich) in sterile saline at 5 mL kg -1 . Tetrabenazine (Sigma Aldrich) was dissolved in 10 % DMSO (Sigma Aldrich) in saline with 1 M HCl (10 µL mL -1 injection volume). All solutions were made fresh at the time of injection and all injections were given via intraperitoneal (i.p.) injection. Urethane (Sigma Aldrich) was dissolved in sterile saline as a 25 % w/v solution and administered at 7 µL kg -1 .
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