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Axiovert observer z1 inverted fluorescence microscope

Manufactured by Zeiss
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The Axiovert Observer Z1 is an inverted fluorescence microscope designed for life science research. It features a high-resolution optical system and advanced imaging capabilities. The microscope is equipped with fluorescence illumination and can be used for a variety of imaging applications.

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Lab products found in correlation

Cell tak adhesive, by Corning (2 mentions) Axiovision 4, by Zeiss (2 mentions) Axiocam mrm camera, by Zeiss (2 mentions)

Close spelling variants of this product

Axiovert Observer Z1 inverted microscope Observer Z1 inverted fluorescence microscope Axiovert Observer Z1 inverted Axiovert Observer microscope Z1 Observer Z1 inverted microscope Observer Z1 fluorescence microscope Z1 Inverted Fluorescence microscope Axiovert inverted fluorescence microscope Axiovert Observer Z1 Inverted Observer Z1

2 protocols using axiovert observer z1 inverted fluorescence microscope

1

Visualizing Toxoplasma Cysteine Protease Activity

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Brains containing ME49 cysts were harvested from infected mice and homogenized in 1 ml of 1X PBS by sequential syringing through 19G and 23G. Homogenized brain material were then incubated for 30 min with either 200 nM BODIPY-LHVS (BO-LHVS) alone or a 1:1 or 1:10 mixture of BO-LHVS and LHVS. After three washes with 1X PBS, brain material was fixed with 4% paraformaldehyde and stained with rabbit anti-TgCPL antibodies at a 1:200 dilution followed by Alexa 594-conjugated goat anti-rabbit antibodies. Stained brain material was then allowed to adhere on slides coated with Cell-tak adhesive (Corning). Images were captured with a Zeiss Axiovert Observer Z1 inverted fluorescence microscope and an AxioCAM MRm camera and processed using Zeiss Axiovision 4.3 software.
Di Cristina M., Dou Z., Lunghi M., Kannan G., Huynh M.H., McGovern O.L., Schultz T.L., Schultz A.J., Miller A.J., Hayes B.M., van der Linden W., Emiliani C., Bogyo M., Besteiro S., Coppens I, & Carruthers V.B. (2017). Toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection. Nature microbiology, 2, 17096.
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2

Visualizing Toxoplasma Cysteine Protease Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brains containing ME49 cysts were harvested from infected mice and homogenized in 1 ml of 1X PBS by sequential syringing through 19G and 23G. Homogenized brain material were then incubated for 30 min with either 200 nM BODIPY-LHVS (BO-LHVS) alone or a 1:1 or 1:10 mixture of BO-LHVS and LHVS. After three washes with 1X PBS, brain material was fixed with 4% paraformaldehyde and stained with rabbit anti-TgCPL antibodies at a 1:200 dilution followed by Alexa 594-conjugated goat anti-rabbit antibodies. Stained brain material was then allowed to adhere on slides coated with Cell-tak adhesive (Corning). Images were captured with a Zeiss Axiovert Observer Z1 inverted fluorescence microscope and an AxioCAM MRm camera and processed using Zeiss Axiovision 4.3 software.
Di Cristina M., Dou Z., Lunghi M., Kannan G., Huynh M.H., McGovern O.L., Schultz T.L., Schultz A.J., Miller A.J., Hayes B.M., van der Linden W., Emiliani C., Bogyo M., Besteiro S., Coppens I, & Carruthers V.B. (2017). Toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection. Nature microbiology, 2, 17096.
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