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The MR766 is a laboratory instrument designed for the cultivation and maintenance of microorganisms. It provides a controlled environment for the growth and preservation of various microbial cultures. The MR766 allows for the precise regulation of temperature, humidity, and other environmental factors essential for the cultivation of microorganisms.

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Lab products found in correlation

Prvabc59, by American Type Culture Collection (5 mentions) Ccl 81, by American Type Culture Collection (4 mentions) Ibh30656, by American Type Culture Collection (4 mentions) Vero cell line, by American Type Culture Collection (2 mentions) Vero cell, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Huh7 cells, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)

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ZIKV MR766 (2 citations)

6 protocols using mr766

1

ZIKV Strain Propagation and Characterization

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African ZIKV strains MR766 (Uganda, 1947) and IbH30656 (Nigeria, 1968), as well as Asian ZIKV strain PRVABC59 (Puerto Rico, 2015) virus stocks were purchased from ATCC. Asian ZIKV strain H/PF/2013 (French Polynesia, 2013) was kindly provided by Dr. Michael Diamond (Washington University School of Medicine). All ZIKV virus stocks were propagated in Vero cell line (ATCC CCL-81, ATCC) and supernatant was harvested at 3–5 dpi. The viral titers were determined by plaque assays on Vero cells. Vero cells were authenticated by morphology check and growth curve analysis, and validated to be mycoplasma-free using Hoechst staining.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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2

Zika Virus Strain Propagation and Characterization

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African ZIKV strains MR766 (ATCC; Passage 5; Vero cells; Uganda, 1947) and IbH30656 (ATCC; Passage 2; Vero cells; Nigeria, 1968), as well as Asian ZIKV strain PRVABC59 (ATCC; Passage 2; Vero cells; Puerto Rico, 2015) virus stocks were purchased from ATCC. Asian ZIKV strain H/PF/2013 (Passage 2; Vero cells; French Polynesia, 2013) was kindly provided by Dr. Michael Diamond (Washington University School of Medicine). All ZIKV virus stocks were propagated in Vero cell line (ATCC CCL-81, ATCC) and supernatant was harvested at 3–5 dpi. The viral titers were determined by plaque assays on Vero cells. Vero cells were authenticated by morphology check, growth curve analysis, and validated to be mycoplasma-free with Hoechst staining.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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3

Zika Virus Infection in Vero Cells

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African green monkey kidney (Vero) cells (American Type Culture Collection, ATCC) were cultured in minimal essential medium (MEM, GIBCO, Gaithersburg, MD, USA) with 10% fetal bovine serum (FBS, GIBCO), 2 mM l-glutamine, 100 U/mL of penicillin and 100 μg/mL of streptomycin, and maintained at 37 °C with 5% CO2.
Two Asian lineage ZIKV strains, PRVABC59 (GenBank: KU501215) and SZ-WIV01 (GenBank: KU963796), and one African lineage ZIKV strain, MR766 (GenBank: LC002520) were used in this study. PRVABC59, originally isolated from the serum sample of a ZIKV-infected patient returning from Puerto Rico in 2015 [33 (link)], was purchased from ATCC. SZ-WIV01, originally isolated from the serum sample of a 38-year-old Chinese male patient who had travelled to Fiji and Samoa in 2016 [45 (link)], was kindly provided by the Academy of Military Medical Sciences of China. MR766, isolated from a sentinel monkey in Uganda in 1947 [46 (link)], was purchased from ATCC. All the three virus strains were passaged for less than five times in Vero cells in our lab. The 50% tissue culture infectious dose (TCID50) of the virus stocks were determined in Vero cells using the Reed–Muench method [47 (link)]. Viral RNA genomes were sequenced and found to be identical to the original ones as shown in https://www.viprbrc.org.
Li S., Armstrong N., Zhao H., Hou W., Liu J., Chen C., Wan J., Wang W., Zhong C., Liu C., Zhu H., Xia N., Cheng T, & Tang Q. (2018). Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System. Viruses, 10(1), 49.
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4

Zika and Dengue Virus Infection Protocols

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HEK-293 cells (CRL-1573) were purchased from the American Type Culture Collection (ATCC). Huh7 cells were obtained from the JCRB Cell Bank. These cells were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (both from HyClone, San Angelo, TX, USA) at 37 °C with 5% CO2 in a humidified incubator. The ZIKV strains PRVABC59 (ATCC VR-1843) and MR766 (ATCC VR-84) were purchased from ATCC. The ZIKV strain H/PF/2013 (001v-EVA1545) was obtained from the European Virus Archive. The ZIKV strain Asian (NCCP43245) and the DENV-4 strain (NCCP43257) were kindly provided by the Korean Centers for Disease Control and Prevention. A chemical library composed of 467,000 structurally diverse chemical compounds was obtained from the Korea Chemical Bank at the KRICT.
Shin H.J., Kim M.H., Lee J.Y., Hwang I., Yoon G.Y., Kim H.S., Kwon Y.C., Ahn D.G., Kim K.D., Kim B.T., Kim S.J, & Kim C. (2021). Structure-Based Virtual Screening: Identification of a Novel NS2B-NS3 Protease Inhibitor with Potent Antiviral Activity against Zika and Dengue Viruses. Microorganisms, 9(3), 545.
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5

ZIKV Strain Propagation and Characterization

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African ZIKV strains MR766 (Uganda, 1947) and IbH30656 (Nigeria, 1968), as well as Asian ZIKV strain PRVABC59 (Puerto Rico, 2015) virus stocks were purchased from ATCC. Asian ZIKV strain H/PF/2013 (French Polynesia, 2013) was kindly provided by Dr. Michael Diamond (Washington University School of Medicine). All ZIKV virus stocks were propagated in Vero cell line (ATCC CCL-81, ATCC) and supernatant was harvested at 3–5 dpi. The viral titers were determined by plaque assays on Vero cells. Vero cells were authenticated by morphology check and growth curve analysis, and validated to be mycoplasma-free using Hoechst staining.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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6

Zika Virus Strain Propagation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
African ZIKV strains MR766 (ATCC; Passage 5; Vero cells; Uganda, 1947) and IbH30656 (ATCC; Passage 2; Vero cells; Nigeria, 1968), as well as Asian ZIKV strain PRVABC59 (ATCC; Passage 2; Vero cells; Puerto Rico, 2015) virus stocks were purchased from ATCC. Asian ZIKV strain H/PF/2013 (Passage 2; Vero cells; French Polynesia, 2013) was kindly provided by Dr. Michael Diamond (Washington University School of Medicine). All ZIKV virus stocks were propagated in Vero cell line (ATCC CCL-81, ATCC) and supernatant was harvested at 3–5 dpi. The viral titers were determined by plaque assays on Vero cells. Vero cells were authenticated by morphology check, growth curve analysis, and validated to be mycoplasma-free with Hoechst staining.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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