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Collagen and thrombin

Manufactured by Chrono-Log
Sourced in Australia, United States

Collagen and thrombin are laboratory-grade reagents used in various biomedical applications. Collagen is a natural protein found in connective tissues, while thrombin is an enzyme involved in the blood clotting process. These two components are often used together in research and clinical settings, but a detailed description of their specific functions and intended uses is not available within the scope of this request.

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2 protocols using collagen and thrombin

1

Blocking Innate Immune Receptors and Inflammatory Mediators

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Purified anti-human TLR2, TLR4 blocking antibodies and their matching isotype controls were obtained from BioLegend, Inc., USA. Bay 61–3606 and Phorbol 12-myristate 13-acetate (PMA), ticagrelor and Cell Detection ELISA PLUS kit were from Sigma-Aldrich, Australia. ML-171, aspirin, RGDS, cathepsin G inhibitor I, neutrophil elastase inhibitor (1-(3-methylbenzoyl)-1H-indazole-3-carbonitrile), myeloperoxidase inhibitor 1 (4-Aminobenzoic acid hydrazide), losartan were obtained from Cayman Chemical, USA. Abciximab (ReoPro) and low molecular weight heparin (Clexane enoxaparin sodium) were from Eli Lilly and Sanofi Aventis Australia Pty Ltd., respectively. DNAse I solution was purchased from STEMCELL Technologies Australia Pty Ltd. Collagen and thrombin were from Chrono-log Corporation, USA. Human PMN Elastase ELISA kit was obtained from Abcam Biotechnology, Cambridge, UK.
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2

Platelet Activation Assay with Matrine

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Matrine was purchased from MedChemExpress (Monmouth Junction, NJ, United States) with a purity ≥98% and dissolved in saline. Collagen-related peptide (CRP) was prepared as previously described (Arthur et al., 2012 (link)). Collagen and thrombin (≥10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, United States). Fibrinogen and BSA (bovine serum albumin) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Mepacrine (also known as Quinacrine) was from APExBIO (Boston, MA, United States). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, United States). PE-labelled anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI antibody were purchased from eBioscience (San Diego, CA, United States). Alexa Fluor-546-labelled phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA, United States). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and HRP-conjugated anti-rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA, United States).
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