S4207

Xenograft tumor experiments were approved by the Animal Care and Use Committee at the University of Pennsylvania. Briefly, ten female NSG mice (the Jackson Laboratory, 6 weeks, 005557) were injected subcutaneously into both flanks with 2 million LPS246 cells stably expressing TetO-FBP2. Before injection, cells were resuspended in 100 μl PBS mixed with an equal volume of Matrigel (Corning, 356234). Once palpable tumors were established, tumor volume was measure with a digital caliper. When the average tumor size reached 100 mm³, ten mice were randomly separated into two groups: (1) Doxycycline diet (Bio-Serv, S3888) and (2) control diet (Bio-Serv, S4207). Upon completion of the experiment, the animals were sacrificed by CO₂ inhalation and xenograft tumors were dissected for downstream analyses. Mice were housed in a controlled environment (12 h light/12 h dark cycle, humidity 30~70%, temperature 20~22°C), and had free access to water and rodent diet. All animal experiments were performed in accordance with the Guide for Care and Use of Laboratory Animals of the NIH. All animal studies were approved by Institutional Animal Care and Use Committee Office of Animal Welfare of University of Pennsylvania.