Silica beads

Manufactured by MP Biomedicals

Sourced in United States

Silica beads are spherical particles made of silica, a naturally occurring mineral compound. They are used as a versatile lab equipment for various applications, such as chromatography, adsorption, and desiccation. The core function of silica beads is to provide a high surface area for interactions and separations in laboratory settings.

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Lab products found in correlation

Sybr green, by Thermo Fisher Scientific (2 mentions) Quantstudio 3, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Iscript, by Bio-Rad (2 mentions) Fastprep 24 tissue and cell homogenizer, by MP Biomedicals (1 mentions) Fastprep 24 5g bead beating grinder and lysis system, by MP Biomedicals (1 mentions) Protease inhibitor cocktail, by Promega (1 mentions) Glass beads, by Bertin Technologies (1 mentions) Dnase digestion, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

0.1 mm silica beads (11 citations) 0.1 mm spherical silica beads (5 citations) LME) glass/zirconia/silica beads Tube (2 citations)

5 protocols using silica beads

Extraction and Purification of Chicken IgY

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The EYP IgY extraction was done as described elsewhere [26 (link)], with some modifications. Briefly, 9 mL of sterile PBS and 3 g of EYP were vigorously mixed and an equal volume of chloroform added. The mixture was briefly homogenized, centrifuged at 4 000 x g for 30 min and the water-soluble fraction, which contained the IgYs, was collected.
Aliquots of each EEYP were frozen at -80°C overnight and mechanically broken using a clean mortar and pestle. A 3 gr fraction was used for EYP IgY extraction, with the addition of silica beads (MP Biomedicals, Solon, OH, USA) and the use of a FastPrep-24 Tissue and Cell Homogenizer (MP Biomedicals,) set at 6.0 m/s for 30 s before the chloroform extraction in order to maximize the release of IgYs.

Soumaila Garba A., Thibodeau A., Perron A., Laurent-Lewandowski S., Letellier A, & Fravalo P. (2019). In vitro efficacy of potentiated egg yolk powder against Campylobacter jejuni does not correlate with in vitro efficacy. PLoS ONE, 14(3), e0212946.

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Quantitative PCR Analysis of Mouse Tissues

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Flash frozen perfused isolated mouse tissues (quadriceps, hippocampus) were placed in plastic tubes with silica beads (MP Biomedical, 116913100) and homogenized in the FastPrep-24 5G bead beating grinder and lysis system (MP Biomedical, 116005500) in TRI-zol (Invitrogen, 15596026). RNA was extracted via standard phenol-chloroform procedures followed by DNase digestion (ThermoFisher, AM2238). cDNA was generated using iScript (Bio-Rad Technologies, 1708891). Quantitative PCR was run using Sybrgreen (Life Technologies, 4309155) for chemical detection (Applied Biosystems; QuantStudio 3). Enrichment was determined based on double-delta CT value. Primers were ordered from IDT Predesigned qPCR Assays unless otherwise specified. Primers used are listed in the key resources table.

Matthews I., Birnbaum A., Gromova A., Huang A.W., Liu K., Liu E.A., Coutinho K., McGraw M., Patterson D.C., Banks M.T., Nobles A.C., Nguyen N., Merrihew G.E., Wang L., Baeuerle E., Fernandez E., Musi N., MacCoss M.J., Miranda H.C., La Spada A.R, & Cortes C.J. (2023). Skeletal muscle TFEB signaling promotes central nervous system function and reduces neuroinflammation during aging and neurodegenerative disease. Cell reports, 42(11), 113436.

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Protein Extraction from Mycobacterium tuberculosis

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Wild-type H37Rv and complemented strains were grown in 50 mL Middlebrook 7H9 10% OADC 0.05% tween-80 containing the proper antibiotics up to an OD₆₀₀ of 0.5–0.7. Cellular pellets were washed twice using 10 mM Tris HCl pH 8.0. Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals). Cells were disrupted by using a L-Beader 3 (Loccus Biotecnologia) at a speed setting of 4000 rpm, 10 cycles of 30 s each, cooling between cycles. After lysis, the cell free supernatants were collected by centrifugation at 2300g for 5 min at room temperature. The supernatants were filtered through 0.22 μm Millex Durapore filters (Millipore). Protein concentrations were measured using the bicinchoninic acid assay (Pierce).

Villela A.D., Eichler P., Pinto A.F., Rodrigues-Junior V., Yates III J.R., Bizarro C.V., Basso L.A, & Santos D.S. (2015). Gene replacement and quantitative mass spectrometry approaches validate guanosine monophosphate synthetase as essential for Mycobacterium tuberculosis growth. Biochemistry and Biophysics Reports, 4, 277-282.

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Cell Disruption Bead Mixtures

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Mixtures of beads used for cell disruption were: (i) “Beads mixture A” (BMA) containing 2/3 silica beads 0.1 mm (MP Biomedicals) and 1/3 glass beads 0.5 mm (Bertin Technologies), (ii) “Beads mixture B” (BMB) containing 2/3 silica beads 0.1 mm and 1/3 glass beads 0.1 mm (Bertin Technologies), and (iii) “Beads mixture C” (BMC) corresponding to 1/3 of each bead types.

Hayoun K., Gouveia D., Grenga L., Pible O., Armengaud J, & Alpha-Bazin B. (2019). Evaluation of Sample Preparation Methods for Fast Proteotyping of Microorganisms by Tandem Mass Spectrometry. Frontiers in Microbiology, 10, 1985.

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Quantifying Gene Expression in Mouse Tissues

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Flash frozen perfused isolated mouse tissues (Quadricep, Hippocampus) were placed in plastic tubes with silica beads (MP Biomedical, 116913100) and homogenized in TRIzol (Invitrogen, 15596026). RNA was extracted via standard phenol-chloroform procedures followed by DNase digestion (Thermo Fisher, AM2238). cDNA was generated using iScript (Bio-Rad Technologies, 1708891). Quantitative PCR was run using Sybrgreen (Life Technologies, 4309155) for chemical detection (Applied Biosystems; QuantStudio 3). Enrichment was determined based on double-delta CT value. Primers were ordered from IDT Predesigned qPCR Assays unless otherwise specified. Primers used are listed in Primer Table 1.

Matthews I., Birnbaum A., Gromova A., Huang A., Liu K., Liu E.A., Coutinho K., McGraw M., Patterson D.C., Banks M.T., Nobles A.C., Nguyen N., Merrihew G.E., Wang L., Baeuerle E., Fernández E., Musi N., MacCoss M.J., Miranda H.C., Spada A.R.L., & Cortés C.J. (2022). Skeletal Muscle TFEB Signaling Promotes Central Nervous System Function and Reduces Neuroinflammation during Aging and Neurodegenerative Disease.

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