P4 2080 plus hplc system

Optical rotation values were obtained on a Jasco P-1010 polarimeter at the sodium D line (589 nm). UV spectra were recorded on an Agilent 8453 spectrophotometer. IR spectra were recorded on a Bruker Tensor 27 FT-IR instrument. NMR spectra were recorded on a Bruker Avance III Spectrometer at 500.13 MHz for ¹H and 125.76 MHz for ¹³C and a Bruker Avance III 400 Spectrometer at 400.13 MHz for ¹H, 100.62 MHz for ¹³C NMR, chemical shifts were referenced to TMS ^δH and ^δC = 0 ppm. DEPT, COSY-45, gTOCSY, gROESY, gHSQC, gHMQC, and gHMBC, spectra were recorded using standard Bruker pulse sequences. Low resolution mass spectra were recorded on a Waters MaldiSynapt instrument (ESI and APPI), a Waters Xevo TQD instrument (ESI), an Aviv Analytical 5975-SMB instrument (GCMS with cold EI) and Agilent Technologies GCMS5977A MDS with 7890B GC system. High resolution mass spectra were recorded on a Waters MaldiSynapt instrument (ESI and APPI) and Bruker Maxis Impact QTOF APGC instrument equipped with a Bruker SCION456 GC. HPLC separations were performed on a Merck Hitachi HPLC system (L-6200 Intelligent pump and L-4200 UV-VIS detector), a JASCO P4-2080 plus HPLC system with a Multiwavelength detector, and an Agilent 1100 Series HPLC system.

Optical rotation values were obtained on a JASCO P-1010 polarimeter at the sodium D line (589 nm). UV spectra were recorded on an Agilent 8453 spectrophotometer. IR spectra were recorded on a Bruker Tensor 27 FT-IR instrument. NMR spectra were recorded on Bruker Avance and Avance III spectrometers at 500.13 MHz for ¹H and 125.76 MHz for ¹³C. DEPT, COSY-45, gTOCSY, gROESY, gHSQC, gHMQC and gHMBC spectra were recorded using standard Bruker pulse sequences. NMR chemical shifts were referenced to TMS δ_H and δ_C = 0 ppm. High-resolution mass spectra were recorded on a Waters MaldiSynapt instrument (ESI) and LC-MS spectra were recorded on a Waters Xevo TQD instrument (ESI). HPLC separations were performed on a Merck Hitachi HPLC system (L-6200 Intelligent pump and L-4200 UV-VIS detector), a JASCO P4-2080 plus HPLC system with a multiwavelength detector and an Agilent 1100 Series HPLC system.

Optical rotation values were obtained on a Jasco P-1010 polarimeter (Jasco, Oklahoma City, OK, USA) at the sodium D line (589 nm). UV spectra were recorded on an Agilent 8453 spectrophotometer (Santa Clara, CA, USA). IR spectra were recorded on a Bruker Tensor 27 FT-IR instrument (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance and Avance III spectrometers (Billerica, MA, USA) at 500.13 MHz for ¹H and 125.76 MHz for ¹³C. DEPT, COSY-45, gTOCSY, gROESY, gHSQC, gHMQC, and gHMBC spectra were recorded using standard Bruker pulse sequences. NMR chemical shifts were referenced to TMS δ_H and δ_C = 0 ppm. High-resolution mass spectra were recorded on a Waters MaldiSynapt instrument (Waters, Milford, MA, USA) (ESI), and LC-MS spectra were recorded on a Waters Xevo TQD instrument (Waters, Milford, MA, USA) (ESI). HPLC separations were performed on a Merck Hitachi HPLC system (l-6200 Intelligent pump and l-4200 UV-VIS detector), a JASCO P4-2080 plus HPLC system with a Multiwavelength detector, and an Agilent 1100 Series HPLC system.