Technical agar

Manufactured by Merck Group

Sourced in United States

Technical agar is a gelling agent derived from red seaweeds. It is used in microbiological and biotechnological applications to prepare solid growth media for culturing microorganisms.

Automatically generated - may contain errors

Lab products found in correlation

Technical agar, by BD (2 mentions) Luria broth lb, by Thermo Fisher Scientific (2 mentions) Mueller hinton broth (mhb), by Merck Group (2 mentions)

3 protocols using technical agar

Synthetic Sputum Agar Plug Preparation

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For the solid agar plugs,
synthetic sputum media 2 (SCFM2) was prepared as reported in literature^{44 (link)} at twice the initial concentration and combined
with 3% technical agar (Sigma-Aldrich).
Solvents and other reagents
were acquired from commercial sources and used as-received, unless
stated otherwise.

Kasza K., Richards B., Jones S., Romero M., Robertson S.N., Hardie K.R., Gurnani P., Cámara M, & Alexander C. (2024). Ciprofloxacin Poly(β-amino ester) Conjugates Enhance Antibiofilm Activity and Slow the Development of Resistance. ACS Applied Materials & Interfaces, 16(5), 5412-5425.

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Culturing and Enumerating E. coli

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The E. coli strains used in this study are listed in Table 1. Bacterial strains were grown at 37 °C with shaking [180 rotations per minute (r.p.m.)] for 18 h to stationary phase in Luria–Broth (LB; Thermo Fisher Scientific, USA). For culture on solid media, bacteria were grown on LB supplemented with 1.5 % technical agar (BD Biosciences, USA). Bacterial c.f.u. were quantified by serial tenfold dilution of bacterial cultures and plating onto Mueller–Hinton broth (MHB; Sigma-Aldrich, USA) supplemented with 1.5 % technical agar. Agar plates were incubated statically in air for 18 h at 37 °C.

Humphrey M., Larrouy-Maumus G.J., Furniss R.C., Mavridou D.A., Sabnis A, & Edwards A.M. (2021). Colistin resistance in Escherichia coli confers protection of the cytoplasmic but not outer membrane from the polymyxin antibiotic. Microbiology, 167(11), 001104.

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E. coli Growth Conditions Optimization

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The E. coli strains used in this study are listed in Table 1. Bacterial strains were grown at 37°C with shaking (180 rotations per minute (r.p.m.)) for 18 hours to stationary phase in Luria Broth (LB; Thermo Fisher Scientific, USA). For culture on solid media, bacteria were grown on LB supplemented with 1.5% technical agar (BD Biosciences, USA). Bacterial colony forming units (c.f.u.) were quantified by serial 10-fold dilution of bacterial cultures and plating onto Mueller-Hinton broth (MHB; Sigma-Aldrich, USA) supplemented with 1.5% technical agar. Agar plates were incubated statically in air for 18 hours at 37°C.

Humphrey M., Larrouy‐Maumus G., Furniss R.C.D., Mavridou D.A., Sabnis A., & Edwards A.M. (2021). Colistin resistance in Escherichia coli confers protection of the cytoplasmic but not outer membrane from the polymyxin antibiotic.

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