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218 protocols using sas stat software

1

Randomized Transcranial Direct Current Stimulation

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The randomization sequence was built using SAS/STAT Software (Statistical Analysis System, version 9.4). Block randomization with different block sizes was used for HD-tDCS3 × 1 condition (anode center, cathode center and sham), and current intensity (1, 2, and 3 mA). Simple balanced randomization within a single randomization block of four was used to determine the sham condition (anode center or cathode center).
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2

Statistical Analysis of Experimental Data

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Statistical analysis was performed using SAS/STAT software (version 9.4 by Statistical Analysis System Corporation, North Carolina, USA). Data were compared using Fisher's exact test and χ2 to evaluate differences between qualitative variables and using a t test to compare quantitative variables. One-way ANOVA was used to determine statistically significant differences between the means of three or more groups. A p value of <0.05 based on a two-tailed analysis was considered significant.
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3

Genetic Association Analysis Protocol

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The Hardy–Weinberg equilibrium (HWE) for genotype distributions was assessed with the χ2-test. Association analyses were carried out by comparing genotype frequencies of each polymorphism in all patient cohorts using SPSS v17.0 and HaploView v4.2 [63 (link)]. To rule out false positive results, an online adjustment tool for multiple correction testing was used (https://multipletesting.com (accessed on 7 October 2020)), enabling us to perform simultaneous Bonferroni, Holm, Hochberg and false discovery rate (FDR) analyses [64 (link)]. Linkage disequilibrium and haplotype analyses were performed with the HaploView v4.2 software (https://mybiosoftware.com/haploview-4-2-analysis-visualization-ld-haplotype-maps.html (accessed on 07 October 2020)). Genotype data of a healthy, Caucasian population for linkage disequilibrium testing were obtained from the 1000 Genomes Browser (https://www.internationalgenome.org/1000-genomes-browsers/index.html (accessed on 7 October 2020)).
Association analysis between PANSS subscale factors was carried out with the General Linear Model (GLM) algorithm of the Statistical Analysis System’s SAS/STAT software version 14.2. ANOVA of the luciferase assays was performed with the GraphPad InStat software (version 3.05).
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4

Randomized Block Design Analysis of Accession Traits

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The experimental data were analyzed using a joint randomized block design analysis as an approximation for simplicity. The analysis of variance was performed using the PROC GLM procedure of the SAS/STAT software (SAS Institute Inc., Cary, NC, USA). The linear model was
where yijk is the phenotype value of the k-th accession for the j-th replication in the i-th environment, μ is the population mean, ti is the effect of the i-th environment, rj(i) is the effect of the j-th replication in the i-th environment, gk is the effect of the k-th accession, (gt)ik is the interaction effect between accession and environment, and εijk is the random error following N(0, σ2). Except that the effect of accession was considered fixed, all other effects were considered random. The trait heritability for the single environment and multiple environments was estimated, respectively, as
where σg2 is the genotype variance, σgt2 is the genotype and year interaction variance, σ2 is the error variance, nt is the number of years, and nr is the number of replications. The variance components were estimated using the PROC MIXED procedure of the SAS/STAT software (SAS Institute Inc., Cary, NC, USA). The genetic coefficient of variation (GCV) was calculated as GCV = σg/μ.
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5

Pediatric Ophthalmologist Ergonomic Insights

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Statistical analysis of collected responses included odds ratios, Pearson Chi-Square exact tests (due to the small counts of some factor combinations), Spearman correlations, and a negative binomial count data model. Statistical analyses were generated with PROC FREQ and PROC LOGISTIC from SAS/STAT software, Version 9.4 (© 2002–2012) of the SAS System for Windows (Cary, NC). The Institutional review board approved this study from the University of Nebraska Medical Center. The study was limited to pediatric ophthalmologists to provide a more homogenous sampling group that can be meaningfully interpreted for ergonomic change and innovation.
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6

Factors Influencing Youth Sports Participation

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Descriptive statistics were used to summarize children’s participation in YS for the primary social epidemiology study (n = 281) and the community trial sub-study (n = 215). Mixed-models were used to analyze the dichotomous outcome (i.e., 1 = Yes; 0 = No) of YS participation, and the continuous outcome of average minutes of daily MVPA. For all models, community was included as a random effect (34 (link)). SAS/STAT software, version 9.4 (© 2002–2012) of the SAS System for Windows (Cary, NC), was used for all analyses. PROC GLIMMIX was performed to examine the dichotomous outcome of YS participation with the fixed effects of grade, sex, ethnoracial status, and family income. Interactions between the effects of grade, sex, ethnoracial status, and family income on YS participation were also explored. PROC MIXED was used to examine average minutes of daily MVPA with the fixed effects of grade, sex, ethnoracial status, family income, and YS participation. Similarly, interaction effects of grade, sex, ethnoracial status, family income, and YS participation on average time in MVPA were examined. Backward elimination of non-significant interactions based on statistical significance of p < 0.05 was used where non-significant higher order interactions were eliminated first, and then the models were refit (57 , 58 ).
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7

Evaluating Dietary Shifts During Lockdown

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Data are represented as number and percentage in parentheses (%) for categorical variables or mean (sd) for continuous variables.
Internal consistency for the nineteen UPFs included in the questionnaire was assessed by computing Cronbach’s α coefficient (considered satisfactory if higher than or equal to 0·70).
One-way χ2 tests were used to assess differences between increased v. reduced consumption during lockdown as compared to before.
Regression models adjusted for age groups and sex were used to estimate the association of the UPF score (dependent variable) with demographic and socio-economic correlates; based on multivariable regression analysis (model 2), variables with P < 0·10 were included in the multivariable-adjusted regression models used to estimate the association of changes in UPF with lockdown-induced factors and diet-related modifications.
Missing data from categorical variables were assigned a missing indicator. For education, marital status, occupational class, number of cohabitants and living area (< 2 % of missing values) missing values were imputed to the cohort-specific modal value. All analyses were also separately performed for each cohort.
Statistical tests were two-sided, and P values < 0·05 were considered to indicate statistical significance.
Data analysis was generated using SAS/STAT software, version 9.4 (SAS Institute Inc.).
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8

Comparative Analysis of HIV Assays

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All data analyses were performed using SAS/STAT software (Cary, NC). Specimens with sufficient sample volume for completion of the CDC algorithm, the cobas HIV-1/HIV-2 Qual test, and alternate NAT (if required) were included in statistical analyses. Positive, negative, and overall percent agreements (PPA, NPA, and OPA) and associated 2-sided 95% Clopper-Pearson exact confidence intervals (CIs) for comparisons between assays and algorithms were calculated for each viral target (HIV-1 and HIV-2).
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9

Forage Effects on Calf Growth

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The data were analyzed using SAS/STAT software (version 9.4, SAS Institute Inc., Cary, NC). Feed intake and growth data were repeatedly measured using a mixed model that included fixed effects of forage, age, and the forage × age interaction and the random effects of the block and calves within the block. A total of 5 blocks were created. One-way ANOVA was used to analyze the fermentation parameters of the rumen and bacterial diversity. Statistically significant differences among groups were evaluated by Duncan's test. Differences were declared to be statistically significant at P < 0.05. Least squares means with the standard error of the means are reported.
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10

Interrater Agreement on Auditory Perceptual Ratings

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Descriptive statistics were calculated to describe the sample characteristics. Weighted kappa statistics were utilized to examine the interrater agreement of ordinal auditory perceptual ratings between the two raters. The results were assessed as <0 indicating less than chance agreement, 0.01–0.20 as slight agreement, 0.21–0.40 as fair agreement, 0.41–0.60 as moderate agreement, 0.61–0.80 as substantial agreement, and 0.81–0.99 as almost perfect agreement [19 (link)]. Multiple regression analysis models were used to assess the relationships among each voice protocol variable as the outcome and each auditory-perceptual feature as the predictor controlling for sex and age at assessment. The analyses were conducted using SAS/STAT software (version 9.4, SAS System for Windows, SAS Institute Inc., Cary, NC, USA, 2012). All analyses were two-tailed with a p < 0.05 deemed as statistically significant.
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