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5 protocols using sup706

1

3D Printed Biomedical Device Fabrication

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Veroclear and SUP706, used for 3D printing, were purchased from Stratasys (Rehovot, Israel). The medical epoxy (Epo‐Tek 301) was obtained from Epoxy Technology (MA, USA). The magnets were obtained from Daehan Magnet (Seoul, Korea). Titanium membranes with a thickness of 2 μm were purchased from Nilaco (Tokyo, Japan). Acetonitrile (ACN) was obtained from Daejung (Siheung, Korea). Phosphate buffered saline (PBS) and formalin were purchased from Thermo Fisher Scientific (MA, USA). Epinephrine, trifluoroacetic acid (TFA), and acetaminophen were purchased from Sigma‐Aldrich (MO, USA). Glucagon was obtained from Novo Nordisk (Bagsværd, Denmark). The adrenaline/Epinephrine enzyme‐linked immunoassay (ELISA) kit was purchased from MyBioSource (CA, USA). The rat Glucagon enzyme immunoassay (EIA) kit was obtained from RayBiotech (GA, USA). Gentamicin was purchased from Shinpoong (Seoul, South Korea).
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2

Customized Microfluidic Insulin Delivery

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Veroclear and SUP706 were purchased from Stratasys (Rehovot) as 3D‐printing and supporting materials, respectively. The stainless‐steel springs were purchased from Tohatsu. Polyurethane films, 100 μm in thickness, and Teflon molds, were obtained from CY International and 3DMD, respectively. Rubber piston stoppers were obtained by disassembly from a 0.3 ml BD ultra‐fine insulin syringe. Medical epoxy (Epo‐tek 301) was purchased from Epoxy Technology. Phosphate‐buffered saline (PBS) and formalin were obtained from Thermo Fisher Scientific. The check valves were purchased from Minivalve. Trifluoracetic acid (TFA) was purchased from Sigma‐Aldrich. Exenatide (MW = 4187 Da) was purchased from Cosmogenetech. Both insulin and glucagon were obtained from NovoNordisk.
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3

3D Printing of Mandibular Models

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An Objet30 Prime (Stratasys, Ltd., Eden Prairie, MN, USA) 3D printer was used to produce the 10 mandibular models with MJ technology. The chosen materials were a white photopolymer resin VeroWhite (Stratasys, Ltd., Eden Prairie, MN, USA) and a water-soluble support material SUP706 (Stratasys, Ltd., Eden Prairie, MN, USA). The software Objet Studio Software v. 9.2.11.6825 (Stratasys, Ltd., Eden Prairie, MN, USA) was adjusted to the following printing settings with a tray material high-speed (HS), glossy surface option and a layer thickness of 28 microns. The model was positioned in the upper left corner of the built platform. The printing time was 12 h and 14 min. Subsequently, post-processing was required to remove the water-soluble supporting structures with a WaterJet Station (Stratasys, Ltd., Eden Prairie, MN, USA).
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4

3D-Printed Metoprolol Delivery Device

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The clock movements were purchased from Perrin H&W (Ontario, Canada). Veroclear and SUP706 were purchased from Stratasys (Rehovot, Israel) for 3D printing and supporting materials, respectively. The stainless steel springs and polyurethane films were obtained from Misumi (Seoul, Korea) and CY international (Delhi, India), respectively. The medical epoxy (Epo-tek 301) was purchased from Epoxy Technology (MA, USA). The check valves (DU032.002-154.01) and rubber septa (5182-0550) were purchased from Minivalve (OH, USA) and Agilent Technologies (CA, USA), respectively. Metoprolol was purchased from Tokyo Chemical Industry (Chuo-ku, Japan). PBS and formalin were obtained from Thermo Fisher Scientific (MA, USA). Methanol and formic acid were purchased from Daejung (Siheung, Korea) and Sigma-Aldrich (MO, USA), respectively.
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5

Additive Manufacturing of 3D Tumor Phantoms

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The CAD models were translated to the printer in a printable Standard Tessellation Language (STL) format. First, the STLs of the 11 cubic samples (C00–C100) were transferred to the PolyJetTM printer Connex3 Objet500 (Stratasys Ltd., EdenPrairie, MN, USA). Then, 11-cubes in sets of two were printed, each set with a different material for the grid structure: solid bio-compatible MED610 (Stratasys, EdenPrairie, MN, USA) with a Shore hardness of 83-86 (Scale D) and flexible TangoPlus (Stratasys Ltd., EdenPrairie, MN, USA) with rubber-like properties with a Shore hardness of 26-28 (Scale A). SUP706 (Stratasys Ltd., Eden Prairie, MN) was chosen as the support material. The samples were printed with different matrix thicknesses as designed in the CAD software, yielding a specific partial volume contribution C% of the MRI-signal generating material to the voxel.
The spherical tumour models were printed using a different material for the grid structure, TangoPlus, VeroClear and VeroMagenta (Stratasys Ltd.). The general properties of VeroClear and VeroMagenta were similar to those of MED610 but not approved biocompatible. The same support material as for the cubic samples, SUP706, was used.
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