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2 protocols using antibodies to fibronectin

1

Antibody Validation for Signaling Pathways

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Antibodies to H3K27me3, JMJD3, p-Smad3, Smad3, p-AKT, AKT, p-ERK1/2, ERK1/2, Notch1, Jagged-1, UTX and DNMT1 were purchased from Cell Signaling Technology (MA, USA). Antibodies to fibronectin, α-SMA, collagen III, JMJD3, TGF-β1, Notch3 were purchased from Abcam (MA, USA). An antibody to Smad7 was obtained from Santa Cruz Biotechnology (CA, USA). An antibody to β-actin, PTEN and FBXW7 were obtained from proteintech (Wuhan, China). β-tubulin was purchased from Sigma-Aldrich (MO, USA). GSKJ4 was purchased from Selleck (Houston, USA). DMSO and other chemicals were obtained from Beyotime (Shanghai, China). Lipofectamine 3000 was obtained from Invitrogen-Thermo Fisher Scientific (CA, USA).
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2

Characterization of Epithelial-Mesenchymal Transition in Liver Cells

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Following reagents (suppliers) were used in this study: apatinib (Selleck, Texas, USA); hepatocyte growth factor (HGF) (PeproTech, Suzhou, China); antibodies to CD68, ZO-1, N-cadherin, vimentin, Slug and β-actin (Santa Cruz Biotechnology, USA); antibodies to fibronectin, E-cadherin, Snail, p-Met, Met (Abcam, Cambridge, MA). HRP-conjugated secondary antibodies (Vazyme, Nanjing, China). ELISA kit for HGF, EGF, TGF-β1 and IL-6 (R&D Systems, Minneapolis, MN).
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