We collected 15 residual specimens of a positive blood culture bottle (BacT/Alert® FA or BacT/Alert® FN) from adult patients with bacteremia at Toyama University Hospital after the detection of bacterial growth with the BacT/ALERT 3D system (bioMerieux, Inc., Mercy-l’Etoile, France). All specimens were confirmed to be monomicrobial with Gram staining before use, because conventional antimicrobial susceptibility (and even identification) had not been completed at the time of enrollment. However, they were eventually confirmed to be monomicrobial using solid media at the Clinical Laboratory Center (certified ISO15189) at Toyama University Hospital. The MIC values determined by the conventional method, as reference values, were determined with the MicroScan WalkAway system (Siemens Healthcare Diagnostics, IL, USA) in the clinical laboratory.
Bact alert fn
Manufactured by bioMérieux
Sourced in France, United States
The BacT/Alert® FN is a blood culture system designed to detect microorganisms in blood samples. It uses colorimetric sensors to monitor the growth of microorganisms and provides a rapid and automated detection of positive blood cultures.
Automatically generated - may contain errors
Lab products found in correlation
Bact alert fa, by bioMérieux (5 mentions)
Bact alert pf, by bioMérieux (2 mentions)
Bact alert 3d system, by bioMérieux (1 mentions)
Microscan walkaway system, by Siemens (1 mentions)
Microflex lt, by Bruker (1 mentions)
Maldi tof ms, by Bruker (1 mentions)
Bactec plus aerobic f, by BD (1 mentions)
Vitek ms, by bioMérieux (1 mentions)
Bact alert 3d 120, by bioMérieux (1 mentions)
8 protocols using bact alert fn
1
Evaluating Antimicrobial Susceptibility of Bacteremia
2
Rapid Blood Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 10 mL aliquot of healthy venous blood was added to both aerobic and anaerobic bottles. We used BacT/Alert® FA (bioMerieux, Durham, NC, USA) as an aerobic bottle and BacT/Alert® FN (bioMerieux) as an anaerobic bottle. These bottles were incubated for 6 or 24 h at 35 °C. After incubation, the healthy culture-negative specimens were processed with the same procedures as clinical specimens as described below, except for reaction time (for 1 h rather than 2 h) with the ATP-eliminating reagent.
3
Sterility Testing of CIKs Supernatant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sterility test was performed on cellular supernatants at the end of CIKs expansion. Briefly, 10 ml and 4 ml of supernatant respectively for anaerobic and aerobic microorganisms and fungal/yeasts species, were inoculated within the Bact-ALERT® FN and Bact-ALERT® PF (Biomérieux, INC. Durham, NC). The sample was then analyzed by the Laboratory of Bacteriology and Virology—Pediatric Clinical Pathology of City of Health and Science of Turin. The results were available after 7–8 days of incubation.
4
Sepsis Diagnosis with Blood Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, an episode was defined as each separate case of clinically suspected community-onset sepsis treated with intravenous antibiotics. Only one episode per patient and admission were included in the final data analysis to avoid bias. For each episode, two sets of blood cultures from two different puncture sites were collected before administration of the first dose of intravenous antibiotics. However, for a few episodes, due to feasibility, only one set of blood culture was collected. Blood cultures were conducted in BacT/ALERT® FN (bioMérieux, France). Typing and definite species identification with MALDI-TOF MS was performed on a Microflex LT mass spectrometer (Bruker Daltonics, United States) with BioTyper software v2.0 using default parameter settings. Spectral scores above 2.0 were used as cut-off for correct identification. Antibiotic susceptibility was determined by accredited laboratory methods according to EUCAST guidelines (www.eucast.org ).
5
Synovial Fluid Bacterial Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extracted synovial fluid samples were sent to the laboratory department of our hospital for routine aerobic and anaerobic bacterial culture for 7 days. (1) Aerobic culture: the specimens were inoculated on solid AGAR plates (containing goat blood) (Wenzhou Kangtai Biological Technology Co., LTD.) and broth medium (Wenzhou Kangtai Biological Technology Co., LTD.), and the plates were cultured in a constant temperature incubator at 37°C. (2) Anaerobic culture: synovial fluid samples were inoculated into anaerobic blood culture bottles (BacT/AlerT FN, Biomerieux, France), which were placed in the Biomerieux BacT/Alert 3D microbial culture system. All synovial fluid samples were identified by Biomrieux Compact‐2 automatic microbial identification drug susceptibility system and Bruker MALDI‐TOF MS identification mass spectrometry.
6
Blood Culture Media Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, we used the following commonly used commercial blood culture media: BACTEC PLUS Aerobic/F and BACTEC lytic/10 anaerobic/F (BD Diagnostics, Sparks, MD, USA), BacT/Alert FA and BacT/Alert FN (bioMerieux, Durham, NC, USA), and VersaTREK REDOX 1 and REDOX2 (80 mL; TREK Diagnostic Systems, Cleveland, OH, USA). Each type of bottle used in the analyses was from the same batch. The human blood used in the study was purchased from the Red Cross Blood Center of Henan Province and was drawn not more than 5 days prior to use and was stored at 4°C.
7
Anaerobic Bacteroides fragilis Identification and Susceptibility
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood cultures were obtained by general venipuncture guidelines to draw no less than 10 ml of blood when possible. Blood was injected to BacT/ALERT FA and BacT/ALERT FN (bioMérieux, Marcy-l'Etoile, France) bottles. The positive blood cultures were inoculated on Brucella agar and phenylethyl alcohol blood agar, and then incubated in an anaerobic chamber (Forma Scientific, Marietta, OH, USA) at 35℃ for 48 hours. All isolates were identified by either conventional methods [8 ], the Vitek Anaerobe and Corynebacterium (ANC) identification card (bioMérieux), or the VITEK MS (bioMérieux) matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) system. An episode with positive culture of Propionibacterium acnes was excluded, which was considered as a common skin commensal. The antimicrobial susceptibility testing was performed for 44 isolates of the Bacteroides fragilis group following the CLSI agar dilution method [9 ].
8
Automated Blood Culture Monitoring Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All blood cultures were received as per routine work from all wards and ICU of the hospital covered by the Medical Microbiology Laboratory of the PLA. Per routine processes, 8-10mL blood from the adult patient was injected into the blood culture bottle BACT/ALERT® FA and BACT/ALERT® FN (BioMérieux, Shanghai, CN), respectively, and 3-5ml venous blood from pediatric patient were injected into the special BacT/ALERT® PF blood culture bottles .
The blood cultures were then loaded into the automated blood culture continuous monitoring system，BacT-ALERT® 3D 120 (BioMérieux, Shanghai，CN). If no signal was received from a blood culture bottle after seven days, the bottle was retrieved and resulted as 'no growth after seven days (except for the samples which the doctor asks for extending culture time). If the instrument alarm indicated the bottle had possible positive results, it would then be taken out from the instrument for smear and optical microscopy examination. If there were no bacteria found in the microscope field, the bottle would be loaded into the instrument for more culturing until the positive alarm show up again in next seven day, or it will be reported as "no growth".
The blood cultures were then loaded into the automated blood culture continuous monitoring system，BacT-ALERT® 3D 120 (BioMérieux, Shanghai，CN). If no signal was received from a blood culture bottle after seven days, the bottle was retrieved and resulted as 'no growth after seven days (except for the samples which the doctor asks for extending culture time). If the instrument alarm indicated the bottle had possible positive results, it would then be taken out from the instrument for smear and optical microscopy examination. If there were no bacteria found in the microscope field, the bottle would be loaded into the instrument for more culturing until the positive alarm show up again in next seven day, or it will be reported as "no growth".
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!