Td 88137

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Sourced in United States, United Kingdom, Montenegro

The TD.88137 is a laboratory equipment product from Inotiv. It is designed for use in scientific research and analysis applications. The core function of the TD.88137 is to perform a specific task or measurement, but a detailed description is not available while maintaining an unbiased and factual approach.

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TD. 88137 Western Diet (3 citations) Harlan TD.88137 (3 citations) TD.88137 Adjusted Calories Diet (2 citations)

305 protocols using td 88137

Aortic Valve Injury Model in Mice

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The animal studies were approved by the Animal Ethics Committee of Ren Ji Hospital of Shanghai Jiao Tong University School of Medicine and carried out in accordance with the NIH guidelines (Guide for the care and use of laboratory animals). Adult male C57BL/6J mice and male ApoE^–/– mice (C57BL/6J background) aged 6–8 weeks were purchased from GemPharmatech (Jiangsu, China), housed in a pathogen-free, temperature-controlled environment under a 12:12 h light-dark cycle.
Aortic-valve injury was induced as previously described with minor modification (10 (link), 17 (link)). Briefly, the mice were anesthetized by intraperitoneal injection of a solution comprising 150 mg/kg ketamine and 16 mg/kg xylazine, and a spring wire was introduced into the carotid artery. The wire was passed over the aortic valve under echocardiography guidance and rotated over the valve 110 times. Additionally, the leaflets were scratched with the body of the wire 30 times. The carotid artery was ligated before closing the skin incision. Sham surgery was performed in the same way without advancing the wire across the aortic valve into the left ventricle. After surgery, C57BL/6J mice received normal chow or a Western diet (TD88137; Harlan Teklad), ApoE^–/– mice fed with a Western diet (TD88137; Harlan Teklad).

Wen D., Hu L., Shan J., Zhang H., Hu L., Yuan A., Pu J, & Xue S. (2023). Mechanical injury accentuates lipid deposition in ApoE–/– mice and advance aortic valve stenosis: A novel modified aortic valve stenosis model. Frontiers in Cardiovascular Medicine, 10, 1119746.

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Lupus Pathogenesis in Female Mice

Cited 1 time

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All experimental procedures were approved in advance by the University of Nebraska Medical Center's Institutional Animal Care and Use Committee. Lupus is a chronic autoimmune disease that affects more women than men and is characterized by a 9:1 female‐to‐male ratio (Rider et al., 2018 (link)). Hence, based on the sex bias, the present study was limited to female mice. Female FVB/N mice were purchased from Jackson Laboratories (Bar Harbor, ME, Cat# JAX:001800; RRID: IMSR_JAX:001800), and n = 6–8 per group were housed on corncob bedding on a 12‐h light/dark cycle. Commencing at 12 weeks of age, mice were fed either a high‐fat “Western” diet (HFD; fat 42% kcal, sucrose 34% kcal; Cat# TD88137, Teklad, USA) or a control diet (fat 12.6% kcal, sucrose 34% kcal; Cat# TD05230, Teklad, USA), which was a modified version of the TD88137 diet with matched sucrose level. Both diets contained casein as a protein source. Except when mice were housed in metabolic cages for 24 h urine collection, mice were group housed in standard micro isolator cages.

Kakalij R.M., Dsouza D.L., Ha L, & Boesen E.I. (2024). TLR7 activation by imiquimod worsens glycemic control in female FVB/N mice consuming a high‐fat diet. Physiological Reports, 12(3), e15949.

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Angiotensin II-Induced Aortic Aneurysm Model

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Fifteen male low density-lipoprotein receptor-deficient (LDLr -/-) mice (2 months of age, on a C57BL/6 background) were obtained from the Jackson Laboratory (Bar Harbor, ME). Eleven mice were used for aneurysm studies while four other mice were used as healthy age-matched controls. Aneurysms were induced by systemic infusion of angiotensin II (AngII, Bachem Americas, Torrance, CA) in combination with a diet with saturated fat (21% wt/wt) and cholesterol (0.2% wt/wt; catalog no. TD88137; Harlan Teklad) 13 (link). Briefly, mice were fed with high-fat diet for one week before, and six weeks during AngII infusion. Osmotic pumps (model 2004; Alzet, Cupertino, CA) filled with AngII were implanted subcutaneously through an incision at the right back shoulder of the mice. 2% to 3% isoflurane was inhaled by the mice as anesthesia throughout the surgical process. The pumping rate for AngII was set to 1000ng/kg/min. Pumps were explanted four weeks after the implantation and mice were allowed to recover for two weeks. Disease progression was monitored with a high-frequency ultrasound machine, Fujifilm VisualSonics Vevo 2100 (Fujifilm VisualSonics, Toronto, ON, Canada), by utilizing a linear array probe (MS-550D, broadband frequency 22 MHz -55 MHz). All animal use protocols for the study were approved by the Institutional Animal Care and Use Committee (IACUC) at Clemson University.

Wang X., Lane B.A., Eberth J.F., Lessner S.M, & Vyavahare N.R. (2019). Gold nanoparticles that target degraded elastin improve imaging and rupture prediction in an AngII mediated mouse model of abdominal aortic aneurysm. Theranostics, 9(14), 4156-4167.

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Isolation of Adipose Preadipocytes from Mice

Cited 2 times

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C57BL/6J mice (9-weeks-old) were kept in a pathogen-free facility and maintained under a 12 h light–dark cycle at 22° C. 3 mice were fed ad libitum with a high-fat diet (HFD; TD88137 Harlan Teklad) and 3 mice were fed ad libitum with a regular diet (RD). VAT were excised and isolation of preadipocytes [63 (link)]. Animal care and experimental procedures were approved by the Ethics Committee in Animal Experimentation of West China Hospital, Sichuan University, Chengdu, China (record #: 2019014A).
In the GEO database GSE28598 data set, Smad3+/+ mice (WT) and Smad3−/− mice (KO) were fed with a regular diet (RD) or 55% high fat diet (HFD) for 8 weeks. Diet-induced obese (DIO) mice were intraperitoneally injected with 1.5 mg/kg body weight of control 13C4 antibody (IgG) or anti-TGF-β antibody (1D11) three times a week for 8 weeks [22 (link)].

Mao R., Yang F., Zhang Y., Liu H., Guo P., Liu Y, & Zhang T. (2021). High expression of CD52 in adipocytes: a potential therapeutic target for obesity with type 2 diabetes. Aging (Albany NY), 13(8), 11043-11060.

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Metabolic Impact of Genomic Deletion in Mice

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All mouse work was performed under the approved guidelines of the Emory University IACUC. All studies were performed on heterozygous male and female C57BL/6N- Del16^+/Bdh1-Tfrc (B6.Del16^+/Bdh1-Tfrc, MGI:6241487) mice and wild type (WT) littermates [40 ]. All breeding was between B6.Del16^+/Bdh1-Tfrc and WT C57BL/6 N (Charles River Laboratories). Starting at postnatal day 21, mice were fed either a standard diet (STD, LabDiet 5001) low in fat (13.4% energy from fat) or a high-fat diet (HFD, Teklad TD.88137, 42.0% energy from fat) for the remainder of their lives. No method of randomization was used to assign the diet challenge; the random assignment of mice to experimental groups (WT or B6.Del16^+/Bdh1-Tfrc) was based on Mendelian inheritance. No statistical method was used to estimate sample size. Body weight was monitored weekly from 1–16 weeks of age. Indirect calorimetry and behavioral assays were performed on mice between 16–20 weeks of age. At the conclusion of indirect calorimetry, mice were euthanized, and liver tissue was collected for metabolomics analysis. An independent cohort of mice were euthanized and brain tissue was collected and immediately weighed to calculate the brain:body weight ratio. Mice were not fasted prior to euthanasia and tissue collection. The number of animals used in experiments is indicated in figure legends.

Pollak R.M., Purcell R.H., Rutkowski T.P., Malone T., Pachura K.J., Bassell G.J., Epstein M.P., Dawson P.A., Smith M.R., Jones D.P., Zwick M.E., Warren S.T., Caspary T., Weinshenker D, & Mulle J.G. (2022). Metabolic effects of the schizophrenia-associated 3q29 deletion. Translational Psychiatry, 12, 66.

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Dietary Effects on Mouse Metabolism

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The mice used in this study were the progeny of LG/J, SM/J and C57BL/6J animals obtained from the Jackson Labs. Pups from each strain were weaned at three weeks of age and then separated into sex-specific cages of no more than five animals per cage. At this time, one-half of the animals from each litter were fed a high-fat diet (42% calories from fat, TD88137, Harlan Teklad, Madison WI) and one-half were fed a relatively low-fat diet (15% calories from fat, D12284, Research Diets, New Brunswick, NJ, or 13% calories from fat, 5001, Laboratory Rodent Diet, St Louis MO). Animals’ assignment to their respective diet was random. The number of animals used in this study was determined by the number of mice required to have reproducible results and robust metabolic assays.
At 20 weeks of age, animals were fasted for 4h and anesthetized with an overdose of Ketamine/Xylazine cocktail. Blood was collected from the retro-orbital sinus and euthanasia was achieved by cardiac perfusion with PBS in fully anesthetized animals. Serum was frozen at −80°C until assayed. Adipose and liver tissues were flash frozen and stored at −80°C until assayed.

Lawson H.A., Zayed M., Wayhart J.P., Fabbrini E., Love-Gregory L., Klein S, & Semenkovich C.F. (2017). Physiologic and Genetic Evidence Links Hemopexin to Triglycerides in Mice and Humans. International journal of obesity (2005), 41(4), 631-638.

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Atherosclerosis in Ogg1/Nlrp3 Deficient Mice

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Ogg1^−/− mice were kindly provided from Dr. Christi A. Walter (Univ. Texas Health Science Center at San Antonio). Nlrp3^−/− mice were provided by Dr. K. A. Fitzgerald (University of Massachusetts Medical School, Worcester, MA). We have crossed for at least 8 generations and established Ogg1^−/−Ldlr^−/− and Ogg1^−/−Nlrp3^−/− DKO mice. Starting at 8 weeks of age, mice were fed a western diet (WD) (TD88137, Harlan Teklad) for 16 weeks. For bone marrow (BM) transplantation, BM from Ogg^−/−, WT, and Ogg1^−/−Nlrp3^−/− mice was transplanted into irradiated Ldlr^−/− mice. After recovery (8 wks), chimeric mice were placed on a WD for 12 wks. Whole blood was collected to confirm the efficiency of the BM transplant. All animal experimental procedures were conducted in strict compliance with the policies on animal welfare of the National Institute of Health. The protocol was approved by the Animal Care and Use Committee at Cedars-Sinai Medical Center.

Tumurkhuu G., Shimada K., Dagvadorj J., Crother T.R., Zhang W., Luthringer D., Gottlieb R.A., Chen S, & Arditi M. (2016). Ogg1-Dependent DNA Repair Regulates NLRP3 Inflammasome and Prevents Atherosclerosis. Circulation research, 119(6), e76-e90.

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Atherosclerosis Treatment with Vitamin D Depot

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Seven weeks old female ApoE^−/− were fed a high-fat diet (HFD, Harlan Teklad TD.88137, 42% kcal from fat) for 3 months before treatment. To prepare the 1,25-Dihydroxyvitamin D3 (aVD)-loaded FM scaffold, 1,25-Dihydroxyvitamin D3 (0.0067% w/w) (Sigma) was loaded into the polymers (PEG₄₅-b-PPS₄₄ and 20% VS-PEG₄₅-b-PPS₄₄) to form aVD-loaded FMs. The aVD-loaded FMs in PBS were then quickly vortexed with eight-arm PEG-thiol (10% w/v in PBS solution) before use. After 4 months on a high fat diet, 50 μl of various treatment groups were injected s.c. into the mid-scapular region of ApoE^−/− mice every month for 2 months: 1, PBS control; 2, free aVD; 3, aVD-FM-depots. The same amount of aVD (8 μg/kg/month) was used in groups 2 and 3. Mice were kept maintaining on a high-fat diet, and their activities were monitored during treatment.

Yi S., Karabin N.B., Zhu J., Bobbala S., Lyu H., Li S., Liu Y., Frey M., Vincent M, & Scott E.A. (2020). An Injectable Hydrogel Platform for Sustained Delivery of Anti-inflammatory Nanocarriers and Induction of Regulatory T Cells in Atherosclerosis. Frontiers in Bioengineering and Biotechnology, 8, 542.

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Behavioral Studies in High-Fat Diet Mice

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Twelve- to 16-week-old animals were used for behavioral studies. Some mice were used after completing body-weight monitoring and had previously been exposed to a high-fat diet (42.8% calories from fat, TD.88137, Harlan-Teklad). No behavioral differences, however, were noted between chow- and HFD-fed animals and groups (data not shown). Separate cohorts of mice not exposed to a HFD were used for wheel running and operant responding, respectively, so that no mouse included in the other behavioral tasks had been exposed to increase physical activity or calorie restriction. See Supplemental Experimental Procedures for full methodology for behavioral experiments.

Cui H., Lu Y., Khan M.Z., Anderson R.M., McDaniel L., Wilson H.E., Yin T.C., Radley J.J., Pieper A.A, & Lutter M. (2015). Behavioral Disturbances in Estrogen-Related Receptor alpha-Null Mice. Cell reports, 11(3), 344-350.

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Apoe-/- Mice Atherosclerosis Treatment

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In Myh11-CreER^T2-eYFP and Cdh5-CreER^T2-eYFP Apoe^−/− mice, Cre recombinase was activated with a series of ten tamoxifen injections (1mg/day/mouse; Sigma Aldrich, T-5648) over a 2-week period. One week after the tamoxifen treatment, mice were switched from a normal chow diet (Harlan Teklad TD.7012) to a high fat Western-type diet (WD), containing 21% milk fat and 0.15% cholesterol (Harlan Teklad; TD.88137) for 18 weeks followed by 100mg/kg/bw ABT-263 treatment for 6 (2 cycles- 5 days ON and 14 days OFF) weeks or 50mg/kg/bw ABT-263 treatment for 9 (3 cycles- 5 days ON and 14 days OFF) weeks. ABT-263 (S1001) was obtained from Selleck (Houston, TX 77014, USA), formulated in Vehicle (PBS with 15% dimethylsulfoxide and 7% Tween-20) and injected intraperitoneally (IP) at a dose of 100mg/kg/bw or 50mg/kg/bw as shown in the figures, figure legends, and results sections.

Karnewar S., Karnewar V., Shankman L.S, & Owens G.K. (2023). Treatment of advanced atherosclerotic mice with the senolytic agent ABT-263 is associated with reduced indices of plaque stability and increased mortality. bioRxiv.

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