EndoFectin™ Max Transfection Reagent (EFM1004) was purchased from GeneCopoeia, located in Rockville, MD, USA. DEAE-Dextran hydrochloride (#D9885) and Ang II (#A9525) were purchased from Sigma-Aldrich in St. Louis, MO, USA. FMK (#Axon 1848) was purchased from Axon Medchem in VA, USA. Finally, the reagent of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was bought from Sigma-Aldrich Chemie, located in St. Louis, MO, USA.
Reagent of 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a colorimetric assay used to measure cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to purple formazan in the presence of metabolically active cells.
Lab products found in correlation
2 protocols using reagent of 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
Optimizing Cell Transfection Protocols
EndoFectin™ Max Transfection Reagent (EFM1004) was purchased from GeneCopoeia, located in Rockville, MD, USA. DEAE-Dextran hydrochloride (#D9885) and Ang II (#A9525) were purchased from Sigma-Aldrich in St. Louis, MO, USA. FMK (#Axon 1848) was purchased from Axon Medchem in VA, USA. Finally, the reagent of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was bought from Sigma-Aldrich Chemie, located in St. Louis, MO, USA.
Cytotoxicity Evaluation of Breast Cancer Cell Lines
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