Reagent of 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

Manufactured by Merck Group

Sourced in United States

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a colorimetric assay used to measure cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to purple formazan in the presence of metabolically active cells.

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Lab products found in correlation

Acetonitrile, by Avantor (1 mentions) Deae dextran hydrochloride, by Merck Group (1 mentions) Ang 2, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Endofectin max transfection reagent, by GeneCopoeia (1 mentions) Docetaxel, by Selleck Chemicals (1 mentions) Abt 737, by Selleck Chemicals (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Bt474, by American Type Culture Collection (1 mentions) Skbr3, by American Type Culture Collection (1 mentions) Hcc1954, by American Type Culture Collection (1 mentions)

Spelling variants of this product

MTT reagent (974 citations)

2 protocols using reagent of 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

Optimizing Cell Transfection Protocols

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To ensure high-quality and reliable results, the following materials were used in the study. Ultrapure water with a resistivity of 18.2 MΩ cm was obtained from the Mili-Q water purification system (Milford, MA, USA). Acetonitrile, used for HPLC measurements, was of LC-MS grade and purchased from Burdick & Jackson (Muskegon, MI, USA). Formic acid was used as a mobile phase modifier and was of mass-spectrometry grade and obtained from Sigma-Aldrich (Darmstadt, Germany).
EndoFectin™ Max Transfection Reagent (EFM1004) was purchased from GeneCopoeia, located in Rockville, MD, USA. DEAE-Dextran hydrochloride (#D9885) and Ang II (#A9525) were purchased from Sigma-Aldrich in St. Louis, MO, USA. FMK (#Axon 1848) was purchased from Axon Medchem in VA, USA. Finally, the reagent of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was bought from Sigma-Aldrich Chemie, located in St. Louis, MO, USA.

Gao D., Cho C.W., Yang Z., Li X, & Kang J.S. (2023). Identification and Quantitation of the Bioactive Components in Wasted Aralia elata Leaves Extract with Endothelial Protective Activity. Molecules, 28(15), 5907.

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Cytotoxicity Evaluation of Breast Cancer Cell Lines

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Six human breast cancer cell lines including MDA-MB-231, MDA-MB-468, MCF-7, BT-474, HCC-1954, and SK-BR-3 were obtained from the American Type Culture Collection (Rockville, MD, USA) and grown in standard medium supplemented with 10% heat-inactivated fetal bovine serum, 100-IU/mL penicillin, and 100-µg/mL streptomycin. Cell lines were maintained at 37℃ in a humidified atmosphere with 5% CO₂. MCF-7, MDA-MB-231, and MDA-MB-468 cells were cultured in medium additionally supplemented with 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, and 10-µg/mL insulin. ABT-737, docetaxel, and benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (z-VAD-fmk) were purchased from Selleck Chemicals (Houston, TX, USA) and solubilized in dimethyl sulfoxide obtained from Thermo Fisher Scientific (Waltham, MA, USA). Reagent of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Hwang E., Hwang S.H., Kim J., Park J.H., Oh S., Kim Y.A, & Hwang K.T. (2018). ABT-737 ameliorates docetaxel resistance in triple negative breast cancer cell line. Annals of Surgical Treatment and Research, 95(5), 240-248.

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