Changes in size of proteins following the reactions of apoTf and Fe2Tf with Ru complexes were determined by a dynamic light scattering (DLS) technique, using a Malvern ZetaSizer NanoS instrument (173° scattering angle, 298 K) with ZEN0040 disposable cuvettes (Malvern Panalytical, Malvern, UK). Protein samples (0.10 mM) were diluted 10-fold with binding buffer before the measurements. The measured parameters were the averages of 12–15 scans (scan time, 3 s). Urea gel electrophoresis of apoTf and Fe2Tf samples [28 (link)] in the presence of varying concentrations of KP1019 was performed as described previously [29 (link)]. Analysis of Tf crystal structures that are available in the public domain from the Protein Data Bank (PDB) was performed with PyMOL software (version 2.1.1, Schrodinger LLC 2021, New York, NY, USA).
Zen0040 disposable cuvettes
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The ZEN0040 disposable cuvettes are single-use sample containers designed for use with Malvern Panalytical's analytical instruments. They provide a convenient and consistent way to present samples for analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using zen0040 disposable cuvettes
1
DLS and Urea Gel Analysis of Protein Complexes
2
Characterization of Casein Micelles in Skim Milk
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Raw milk samples were skimmed individually by centrifugation at 3,500 rpm for 30 min at 4°C, followed by filtration through glass wool to remove fat, and diluted 1:100 using ultrapure water (18.2 MΩ•cm).
The mean diameter of casein micelles, PDI, and zeta potential of each skim milk (as described above) were determined using a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). The measurement was carried out at 25°C, with a scattering angle of 173° (Visentin et al., 2015) (link) and each measurement had 3 automatic readings. Refractive index for protein was set to 1.45, with absorption coefficient of 0.001; for water, as dispersant, the refractive index was set to 1.33, viscosity was 0.8871 cP, and dielectric constant was 78.5. Sample cells used for casein micelle size and PDI determination were Malvern ZEN0040 disposable cuvettes. A Malvern DTS1070 disposable folded capillary tube was used for zeta potential measurement. These analyses were carried out in triplicate.
The mean diameter of casein micelles, PDI, and zeta potential of each skim milk (as described above) were determined using a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). The measurement was carried out at 25°C, with a scattering angle of 173° (Visentin et al., 2015) (link) and each measurement had 3 automatic readings. Refractive index for protein was set to 1.45, with absorption coefficient of 0.001; for water, as dispersant, the refractive index was set to 1.33, viscosity was 0.8871 cP, and dielectric constant was 78.5. Sample cells used for casein micelle size and PDI determination were Malvern ZEN0040 disposable cuvettes. A Malvern DTS1070 disposable folded capillary tube was used for zeta potential measurement. These analyses were carried out in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!