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Plenti cmv gfp dest

Manufactured by Addgene
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The PLenti CMV-GFP-DEST is a lentiviral vector that allows for the expression of a gene of interest under the control of the CMV promoter. The vector also contains a GFP reporter gene for visual monitoring of transduced cells. This product is suitable for applications requiring lentiviral-mediated gene delivery and expression.

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Lab products found in correlation

Lr clonase, by Thermo Fisher Scientific (2 mentions) Bp clonase, by Thermo Fisher Scientific (2 mentions) Pdonr221, by Thermo Fisher Scientific (2 mentions) Plenti cmv to gfp zeo dest, by Addgene (2 mentions) Fluorochrome conjugated antibody, by BD (1 mentions)

Spelling variants of this product

PLenti-CMV-GFP (7 citations) PLenti CMV/TO GFP-Zeo DEST (2 citations)

3 protocols using plenti cmv gfp dest

1

Comprehensive Immunotherapy Protocol

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Anti-PD-1 antibody (clone J43, BE0033-2), anti-CTLA-4 (clone 9H10, BP0131), anti-Ly6G (clone 1A8, BE0075-1), and IFNAR depleting antibodies (Clone MAR1-5A3, BE0241) were purchased from Bio X Cell (West Lebanon, NH). Fluorochrome-conjugated antibodies were purchased from BD Biosciences (San Jose, CA) and BioLegend (San Diego, CA). pHAGE PGK-GFP-IRES-LUC-W (#46793), Lenti-LucOS (#22777), and pLenti-CMV GFP-DEST (# 19732) were obtained from Addgene. All other chemicals and reagents were from Sigma-Aldrich (St. Louis, MO) unless indicated.
Saddawi-Konefka R., O’Farrell A., Faraji F., Clubb L., Allevato M.M., Jensen S.M., Yung B.S., Wang Z., Wu V.H., Anang N.A., Msari R.A., Schokrpur S., Pietryga I.F., Molinolo A.A., Mesirov J.P., Simon A.B., Fox B.A., Bui J.D., Sharabi A., Cohen E.E., Califano J.A, & Gutkind J.S. (2022). Lymphatic-preserving treatment sequencing with immune checkpoint inhibition unleashes cDC1-dependent antitumor immunity in HNSCC. Nature Communications, 13, 4298.
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2

Lentiviral Expression System Protocols

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All recombinant DNA work was carried out under approval of the Environmental Health Safety Division of UNC-Chapel Hill. Lentiviral expression plasmids were created by E. Campeau and obtained from Addgene: pLenti CMV-GFP-DEST (736-1, Addgene plasmid 19732), pLenti CMV/TO GFP-Zeo DEST (719-1, Addgene 17431). Packaging plasmids psPax2 and pMD2.6 were created by Didier Trono (Addgene plasmids 12259 and 12260). Mouse ORF-eome constructs were acquired from the ATCC I.M.A.G.E Consortium, then cloned into pDONR 221 (Invitrogen) Gateway donor vectors using Clonase BP (Invitrogen). Cloned pDONR 221 vectors were sub-cloned into lentiviral expression vectors by Clonase LR reaction (Invitrogen). Lentiviral backbones were transfected with 1.5 μg packaging plasmids psPAX2 and pMDG2 with 15 μl Lipofectamine 2000 in 6-well plates into HEK293T cells. Viral particles were harvested at 24 and 48 hours post transfection. Viral particles were used to infect target cells with 10 μg/mL Polybrene in antibiotic-free media.
Dunleavey J.M., Xiao L., Thompson J., Kim M.M., Shields J.M., Shelton S.E., Irvin D.M., Brings V.E., Ollila D., Brekken R.A., Dayton P.A., Melero-Martin J.M, & Dudley A.C. (2014). Vascular channels formed by subpopulations of PECAM1+ melanoma cells. Nature communications, 5, 5200.
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3

Lentiviral Expression System Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
All recombinant DNA work was carried out under approval of the Environmental Health Safety Division of UNC-Chapel Hill. Lentiviral expression plasmids were created by E. Campeau and obtained from Addgene: pLenti CMV-GFP-DEST (736-1, Addgene plasmid 19732), pLenti CMV/TO GFP-Zeo DEST (719-1, Addgene 17431). Packaging plasmids psPax2 and pMD2.6 were created by Didier Trono (Addgene plasmids 12259 and 12260). Mouse ORF-eome constructs were acquired from the ATCC I.M.A.G.E Consortium, then cloned into pDONR 221 (Invitrogen) Gateway donor vectors using Clonase BP (Invitrogen). Cloned pDONR 221 vectors were sub-cloned into lentiviral expression vectors by Clonase LR reaction (Invitrogen). Lentiviral backbones were transfected with 1.5 μg packaging plasmids psPAX2 and pMDG2 with 15 μl Lipofectamine 2000 in 6-well plates into HEK293T cells. Viral particles were harvested at 24 and 48 hours post transfection. Viral particles were used to infect target cells with 10 μg/mL Polybrene in antibiotic-free media.
Dunleavey J.M., Xiao L., Thompson J., Kim M.M., Shields J.M., Shelton S.E., Irvin D.M., Brings V.E., Ollila D., Brekken R.A., Dayton P.A., Melero-Martin J.M, & Dudley A.C. (2014). Vascular channels formed by subpopulations of PECAM1+ melanoma cells. Nature communications, 5, 5200.
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