For histological analysis, lungs were inflated with 4% paraformaldehyde or formalin (3.7% formaldehyde) and fixed for 36 h. Fixed paraffin sections were rehydrated, subjected to antigen retrieval, and blocked in Tris-buffered saline (0.1% Triton X-100 containing 1% bovine serum albumin) or protein-free blocking solution (DAKO), and sequentially incubated with specific primary antibodies and biotinylated (DAKO) or Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA). Images were produced with a conventional microscope mounted with a DP71 digital camera (Olympus, Japan), an LSM 710 T-PMT confocal microscope (Carl Zeiss, Germany), and an AXIO Zoom.V16 and ApoTome.2 (Carl Zeiss). Images were processed with equivalent parameters using the ZEN Light Edition software (Carl Zeiss).
Lsm 710 t pmt confocal microscope
Manufactured by Zeiss
Sourced in Japan
The LSM 710 T-PMT confocal microscope is a high-performance optical microscope designed for advanced imaging applications. It features a sensitive photomultiplier tube (T-PMT) detector that enables efficient fluorescence detection. The microscope is capable of producing high-resolution, three-dimensional images of samples by scanning the focused laser beam across the specimen and collecting the emitted fluorescence signals.
Automatically generated - may contain errors
Lab products found in correlation
Zen light edition software, by Zeiss (2 mentions)
Axio zoom v16 and apotome 2, by Zeiss (2 mentions)
Dp71 digital camera, by Olympus (2 mentions)
Protein free blocking solution, by Agilent Technologies (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Biotinylated secondary antibody, by Thermo Fisher Scientific (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
2 protocols using lsm 710 t pmt confocal microscope
1
Histological Analysis of Lung Tissue
2
Histological Analysis of Lung Tissues
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For histological analyses, lungs were inflated with 4% paraformaldehyde or formalin (3.7% formaldehyde) and fixed for 36 h. Fixed paraffin sections were rehydrated, subjected to antigen retrieval, blocked in TBS (0.1% Triton X-100 containing 1% BSA) or DAKO protein-free blocking solution, and sequentially incubated with specific primary antibodies, biotinylated secondary antibodies (DAKO), and the Alexa Fluor system (Invitrogen, Waltham, MA, USA). Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using the in situ Cell Death Detection kit (Roche). Images were produced using a conventional microscope mounted with a DP71 digital camera (Olympus), an LSM 710 T-PMT confocal microscope (Carl Zeiss), and an AXIO Zoom.V16 and ApoTome.2 (Carl Zeiss). Images were processed with equivalent parameters using the ZEN Light Edition software (Carl Zeiss) https://www.zeiss.com.cn/microscopy/products/microscope-software/zen-lite/zen-lite-download.html accessed on 6 October 2023.
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