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Glucose oxidase method

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The glucose oxidase method is a laboratory technique used to measure the concentration of glucose in a sample. It involves the enzymatic conversion of glucose to gluconic acid and hydrogen peroxide, which is then detected and quantified. This method provides a reliable and accurate way to determine glucose levels in various biological samples.

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Lab products found in correlation

Elisa kit, by Phoenix Pharmaceuticals (2 mentions) Glucose analyzer, by Beckman Coulter (2 mentions) Autoanalyzer, by Roche (2 mentions) Radioimmunoassay, by Merck Group (1 mentions) Glucagon, by Merck Group (1 mentions) Nefa hr kit, by Fujifilm (1 mentions) Plasma insulin, by Merck Group (1 mentions) C peptide, by Merck Group (1 mentions) Elisa, by Diapharma (1 mentions) Immulite 2000, by Siemens (1 mentions) Aba 200 atc biochromatic analyzer, by Abbott (1 mentions) Estr us ct, by PerkinElmer (1 mentions) Shbg riact, by PerkinElmer (1 mentions) Access assay, by Beckman Coulter (1 mentions) Taqman, by Thermo Fisher Scientific (1 mentions) Ion exchange high performance liquid chromatography method, by Bio-Rad (1 mentions) Immulite, by Siemens (1 mentions) Enzyme immunoassay, by RayBiotech (1 mentions) Sandwich enzyme linked immunosorbent assay, by RayBiotech (1 mentions) Microparticle enzyme immunoassay, by Abbott (1 mentions)

35 protocols using glucose oxidase method

1

Plasma Glucose and Copeptin Measurements

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Plasma glucose was measured by the glucose oxidase method (Beckman Instruments, Fullerton, California, USA). UUN was measured by the local hospital laboratory using the urease/glutamate dehydrogenase method. Fasting samples were obtained from blood collected in plasma heparin or serum EDTA tubes from volunteers immediately after exiting the chamber and stored at −70 °C. Copeptin was measured on stored samples using a commercially available ELISA kit (Phoenix Pharmaceuticals, Burlingame, California, USA); the intraassay and interassay coefficients of variation were 5.7% and 6.8% respectively
Chang D.C., Basolo A., Piaggi P., Votruba S.B, & Krakoff J. (2019). Hydration biomarkers and copeptin: relationship with ad libitum energy intake, energy expenditure, and metabolic fuel selection. European journal of clinical nutrition, 74(1), 158-166.
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2

Comprehensive Lipid and Glucose Analysis

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All chemical analyses were performed on blood samples obtained after a 12-hour overnight fast and stored at −70°C. Plasma glucose levels were determined in duplicate using a glucose oxidase method (Beckman, Palo Alto, CA). Plasma immunoreactive insulin levels were measured in duplicate using a modification of the double antibody radioimmunoassay technique.62 Total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol and triglyceride levels were determined by standardized methodologies at the Northwest Lipid Research Laboratories.63 (link) LDL relative flotation rate (Rf), which characterizes LDL peak buoyancy, was determined by density gradient ultracentrifugation.64 (link) Hepatic lipase activity, which leads to more atherogenic, smaller, denser LDL,65 (link),66 (link) was measured in plasma after heparin bolus.63 (link)
Barry D.R., Utzschneider K.M., Tong J., Gaba K., Leotta D.F., Brunzell J.D, & Easterling T.R. (2015). Intraabdominal fat, insulin sensitivity, and cardiovascular risk factors in postpartum women with a history of preeclampsia. American journal of obstetrics and gynecology, 213(1), 104.e1-104.11.
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3

Glucose and Insulin Measurement Protocols

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Plasma glucose was measured by either the modified Hoffman method (Technicon Instruments, Tarrytown, NY, USA) or the glucose oxidase method (Beckman Instruments, Fullerton, CA, USA). Plasma insulin concentrations were determined by the Herbert modification [28 (link)] of the Yalow and Berson method [29 (link)] or by automated analysers (Access, Beckman Instruments; Concept 4, ICN Radiochemicals, Costa Mesa, CA, USA). Values from the final later insulin assays were regressed to those of the original assay.
Paddock E., Hohenadel M.G., Piaggi P., Vijayakumar P., Hanson R.L., Knowler W.C., Krakoff J, & Chang D.C. (2017). One-hour and two-hour postload plasma glucose concentrations are comparable predictors of type 2 diabetes mellitus in Southwestern Native Americans. Diabetologia, 60(9), 1704-1711.
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4

Vascular assessment in cardiometabolic patients

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In all groups, we measure two vascular indexes with VASERA VS 1000 Instrument (Fukuda Denshi Japan): ankle/brachial ratio (Winsor Index WI), obtained calculating the oscillometric curve area of systolic peak pressures, and peripheral arterial β-stiffness (cardio/ankle vascular index: CAVI) [20 (link)].
In N1 + N2 patients, dorsal transcutaneous allux skin oximetry and carbon dioxide tension (expressed in mm Hg) were measured with 4 chambers oximetry/laser-doppler PERIMED V 5400 instrument.
For clinic data, blood sample was measured only in the fasting sample, as well as lipids. Blood glucose was determined by glucose oxidase method (Beckman, Fullerton, CA). Glycated hemoglobin (HbA1c; upper normal range 5.8% or 40 mmol/mol with IFCC units) was determined by on-line high-pressure liquid chromatography (HPLC; C-R4A Bio-Rad, Milan, Italy) from capillary blood [21 (link)]. Serum triglycerides (TG), total cholesterol HDL-cholesterol, and LDL-cholesterol were measured with enzymatic method [22 ]. Microalbuminuria was calculated with nephelometric method as albumin/creatinine ratio [23 (link)].
Sambataro M., Seganfreddo E., Canal F., Furlan A., del Pup L., Niero M., Paccagnella A., Gherlinzoni F, & dei Tos A.P. (2014). Prognostic Significance of Circulating and Endothelial Progenitor Cell Markers in Type 2 Diabetic Foot. International Journal of Vascular Medicine, 2014, 589412.
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5

Plasma Glucose and Insulin Measurement

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Plasma glucose was measured by the glucose oxidase method (Beckman Instruments, Fullerton, CA). Plasma insulin concentrations were determined by the Herbert modification of the Yalow and Berson method (31 (link)) or by automated analyzers (Access, Beckman Instruments; Concept 4, ICN Radiochemicals, Costa Mesa, CA, USA). Values from the later insulin assays were regressed to those of the original assay.
Travis K.T., Ando T., Stinson E.J., Krakoff J., Gluck M.E., Piaggi P, & Chang D.C. (2022). Trends in spontaneous physical activity and energy expenditure among adults in a respiratory chamber, 1985 – 2005. Obesity (Silver Spring, Md.), 30(3), 645-654.
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6

Fasting Biomarker Measurement Protocol

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Following an overnight fast, 30 mL of blood was collected by venipuncture into 2 tubes containing EDTA and centrifuged within 30 minutes at 4°C to separate plasma. Total cholesterol and TG concentrations were measured using a Hitachi 704 clinical chemistry analyzer (Boehringer Mannheim Diagnostics, Indianapolis, IN) with reagents supplied by the manufacturer (cholesterol/HP, cat. no. 816302; triglycerides/GPO, cat. no 816370). HDL-C was measured in the clear supernatant following a double precipitation with high-molecular weight dextran sulfate as previously described (24 (link)). Plasma samples were stored at –80°C for analysis of inflammatory biomarkers high sensitivity C-reactive protein (hs-CRP), interleukin-8 (IL-8) and tumor necrosis factor alpha (TNFα) using a Proinflammatory Panel V-Plex Kit (Meso Scale Diagnostics, Inc). Glucose levels were measured by a glucose oxidase method (Beckman Instruments, Fullerton, CA) and insulin by insulin specific double antibody radioimmunoassay using human insulin standards and tracer (Linco, St. Louis, MO) with baseline and post-intervention samples included in the same assay.
Miller M., Sorkin J.D., Mastella L., Sutherland A., Rhyne J., Donnelly P., Simpson K, & Goldberg A.P. (2016). Poly is more Effective than Mono - Unsaturated Fat For dietary management IN the Metabolic Syndrome: The MUFFIN Study. Journal of clinical lipidology, 10(4), 996-1003.
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7

Plasma Glucose and Insulin Assay

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Plasma fasting glucose concentrations were measured by the glucose oxidase method (Beckman Instruments, Fullerton, CA) and plasma fasting insulin concentrations were determined by an automated radioimmunoassay (Concept 4; ICN, Costa Mesa, CA). Fasting insulin concentrations were log-transformed for analysis.
Weise C.M., Thiyyagura P., Reiman E.M., Chen K, & Krakoff J. (2015). A potential role for the midbrain in integrating fat‐free mass determined energy needs: An H2 15O PET study. Human Brain Mapping, 36(6), 2406-2415.
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8

Insulin Sensitivity Measurement Protocol

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Insulin-stimulated glucose uptake (M) was measured as an index of insulin sensitivity. Subjects were provided with all meals for the 2 days preceding the clamp to control nutrient intake. After a 12-h overnight fast, subjects underwent the hyperinsulinemic-euglycemic glucose clamp (26 ,27 (link)) as performed in our laboratory (20 (link)). Insulin was infused at a rate of 555 pmol/m2/min, and M is reported in micromoles of glucose infused per kilogram of fat-free mass per minute (µmol/kg FFM/min). Clamp data were not available for one subject after AEX+WL due to a technical problem. Plasma glucose levels were analyzed at 5-min intervals using the glucose oxidase method (Beckman Instruments, Fullerton, CA). Plasma insulin levels were determined by radioimmunoassay (Millipore, St. Charles, MO). Mean insulin and glucose levels during the clamp were 1,131 ± 32 pmol/L and 5.1 ± 0.1 mmol/L, respectively, and did not differ before and after AEX+WL (P > 0.4).
Prior S.J., Blumenthal J.B., Katzel L.I., Goldberg A.P, & Ryan A.S. (2014). Increased Skeletal Muscle Capillarization After Aerobic Exercise Training and Weight Loss Improves Insulin Sensitivity in Adults With IGT. Diabetes Care, 37(5), 1469-1475.
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9

Biochemical Measurements in Plasma

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Plasma insulin, glucagon, cortisol, and c-peptide (Millipore, St. Louis, USA) were measured by the Vanderbilt Diabetes Research and Training Center’s (DRTC) hormone assay and analytical services core. Plasma glucose was measured using the glucose oxidase method (ref. 22 ; Beckman Instruments). Glycerol and lactate23 (link) and plasma specific activity20 (link) were measured as described previously and free fatty acids were measured using a commercially available kit (NEFA-HR kit; Wako Chemicals; Osaka, Japan).
Gregory J.M., Muldowney J.A., Engelhardt B.G., Tyree R., Marks-Shulman P., Silver H.J., Donahue E.P., Edgerton D.S, & Winnick J.J. (2019). Aerobic exercise training improves hepatic and muscle insulin sensitivity, but reduces splanchnic glucose uptake in obese humans with type 2 diabetes. Nutrition & Diabetes, 9, 25.
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10

Plasma Aβ and APOE Genotyping Protocol

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Participants underwent a 75-gram 2-hour OGTT after an overnight fast. Blood samples were obtained through an intravenous catheter and plasma glucose was measured using the glucose oxidase method (Beckman Instruments, Fullerton, Calif., USA) [17 (link)]. All participants had glucose values measured at 0, 60, and 120 min. For some individuals, insulin was measured via radioimmunoassay as described by Kuzuya et al. [18 (link)] for three time points (202 individuals for fasting insulin, 141 individuals for 60 and 120 min). For those who had fasting insulin, the homeostasis model assessment insulin resistance (HOMA-IR) index was calculated with the following formula: fasting insulin (in μU/ml) × fasting glucose (in mg/dl)/405 [19 (link)].
Plasma Aβ42 was measured with an ELISA technique using a rabbit polyclonal antibody to capture nonspecific Aβ followed by an Aβ42-specific 6E10 mouse monoclonal antibody (Signet Laboratories, Dedham, Mass., USA). APOE testing was conducted by the genotyping core of the University of Washington Alzheimer's Disease Research Center. In brief, DNA was extracted from buffy coat preparations and subjected to PCR amplification with primer sequences and methods as per Hixson and Vernier [20 (link)].
Hanson A.J., Banks W.A., Hernandez Saucedo H, & Craft S. (2016). Apolipoprotein E Genotype and Sex Influence Glucose Tolerance in Older Adults: A Cross-Sectional Study. Dementia and Geriatric Cognitive Disorders EXTRA, 6(1), 78-89.
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