U spect 2 ct scanner

Manufactured by MILabs

Sourced in Netherlands

The U-SPECT-II/CT scanner is a high-performance small-animal imaging system that combines single-photon emission computed tomography (SPECT) and computed tomography (CT) capabilities. It is designed to provide high-resolution and high-sensitivity imaging of small laboratory animals.

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Lab products found in correlation

Ly2510924, by MedChemExpress (1 mentions) Inveon micropet ct, by Siemens (1 mentions) Advanced workstation, by GE Healthcare (1 mentions) Pmod 3, by PMOD Technologies (1 mentions) Gamma counter, by PerkinElmer (1 mentions)

Spelling variants of this product

U-SPECT-II/CT (12 citations) U-SPECT-II (10 citations) U-SPECT+/CT (5 citations) U-CT scanner (2 citations)

12 protocols using u spect 2 ct scanner

Multi-Tracer SPECT/CT Imaging of Tumor Mice

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One hour prior to SPECT/CT imaging, all MPC11- and CT26-bearing BALB/c mice received 300 μCi ¹²⁵I and 300 μCi ^99mTc-duramycin (^99mTc-duramycin) intravenously. Mice were imaged in a USPECT-II/CT scanner (MILabs, Utrecht, the Netherlands). CT was performed for 5 min, followed by SPECT, which simultaneously captured both ^99mTc and ¹²⁵I signals. SPECT data were reconstructed using a standard ^99mTc window and ¹²⁵I window, creating two distinct datasets. Window modes are described as follows: (1) ^99mTc: 20% window, 140 keV (range 126–154 keV); (2) ¹²⁵I: 100% window, 30 keV (range 15–45 keV). Data processing and quantitation of ROI were performed by Imanis Life Sciences (Rochester, MN, USA) using PMOD software (Zurich, Switzerland).

Zhang L., Suksanpaisan L., Jiang H., DeGrado T.R., Russell S.J., Zhao M, & Peng K.W. (2019). Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection. Molecular Therapy Oncolytics, 15, 178-185.

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Multimodal Imaging of Small Animals

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Imaging was performed in the Mayo Clinic Small Animal Imaging Core using a U-SPECT-II/CT scanner (MILabs, Utrecht, The Netherlands)(20 ). Scan-volumes for both the SPECT and CT were selected based on orthogonal optical images provided by integrated webcams. Micro-CT image acquisition was performed in 4 min for normal resolution (169-μm square voxels, 640 slices) at 0.5 mA and 60 kV. Image acquisition time was approximately 20 minutes for SPECT (24 projections at 50 seconds per bed position). All pinholes focus on a single volume in the center of the tube and by using an XYZ stage large volumes up to the entire animal can be scanned at uniform resolution.(21 ) Coregistration of the SPECT and CT images was performed by applying pre-calibrated spatial transformation to the SPECT images to match with the CT images. In order to mask background signal from the stomach, animals were administered 350 μl undiluted barium sulfate (40% w/v, Tagitol V, E-Z-EM, Lake Success, NY, USA) by oral gavage using a 22-gauge plastic feeding tube (Instech Laboratories, Plymouth, PA, USA), as previously described.(22 (link))

Hickey R.D., Mao S.A., Amiot B., Suksanpaisan L., Miller A., Nace R., Glorioso J., Peng K.W., Ikeda Y., Russell S.J, & Nyberg S.L. (2015). Noninvasive 3D imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene. Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 21(4), 442-453.

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Multimodal Imaging of Tumor-Bearing Mice

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PET and CT scans were performed on a Siemens Inveon microPET/CT. SPECT and CT images were obtained using a MILabs U-SPECT-II/CT scanner. Tumor-bearing mice were briefly sedated with isoflurane (2%–2.5% isoflurane in 2 L/min O₂) for intravenous injection of 4 to 7 MBq of [⁶⁸Ga]Ga-BL02, or [⁶⁸Ga]Ga-Pentixafor for PET imaging, or 13.1 to 15.2 MBq of [¹⁷⁷Lu]Lu-BL02 for SPECT imaging. Mice received intraperitoneal injection of 7.5 μg (0.25–0.3 mg/kg) of LY2510924 (MedChemExpress), 15 minutes prior to radiotracer administration as blocking controls. The animals were allowed to roam freely during the uptake period (50 or 110 minutes for PET imaging; 1, 4, 24, or 72 hours for SPECT imaging), after which they were sedated and scanned. The parameters of the CT and PET, and CT and SPECT data acquisitions can be found in the Supplementary Materials and Methods.

Kwon D., Takata K., Zhang Z., Chong L., Fraser B., Zeisler J., Miyata-Takata T., Merkens H., Rousseau J., Aoki T., Kuo H.T., Tan R., Zhang C., Lau J., Villa D., Uribe C.F., Lin K.S., Steidl C, & Benard F. (2022). Targeting Refractory Mantle Cell Lymphoma for Imaging and Therapy Using C-X-C Chemokine Receptor Type 4 Radioligands. Clinical Cancer Research, 28(8), 1628-1639.

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Molecular Weight and Central Delivery

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All animal procedures were conducted in accordance with Radboud University Medical Center animal welfare guidelines. Nine male Sprague–Dawley rats with an average weight of 250 g were anesthetized with 2.5–3 % isoflurane inhalation anesthesia and injected with 150 μl of I-125–labeled cetuximab (146 kDa), etanercept (51 kDa), and anakinra (17 kDa). Drugs with different molecular weights were selected to determine the effect of molecular weight on central delivery. For each drug, two rats were given injections in the area overlying the cervical spine at the C6-C7 level using a 30-gauge needle at a depth of 6 mm as described by Tobinick et al. [16 (link)]. One rat was given an injection in the dorsal tail vein, followed by flushing with 1 ml of saline. Directly after injection, the rats were placed in head-down position for 3 minutes. Five minutes after the injection, single-photon emission computed tomography (SPECT) was performed using a U-SPECT-II/CT scanner (MILabs, Utrecht, the Netherlands). After completion of scanning, which took 20 minutes, all rats were euthanized and bio-distribution was determined.

Roerink M.E., Groen R.J., Franssen G., Lemmers-van de Weem B., Boerman O.C, & van der Meer J.W. (2015). Central delivery of iodine-125–labeled cetuximab, etanercept and anakinra after perispinal injection in rats: possible implications for treating Alzheimer’s disease. Alzheimer's Research & Therapy, 7, 70.

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In vivo aortic angiography in mice

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In vivo micro-computed tomography angiography was under 1.5–2% isoflurane anesthesia using the U-SPECT-II/CT scanner (Milabs, Utrecht, the Netherlands) in a subset of randomly selected mice (3 control Apoe^−/− and 4 TGFβR2^iSMC-Apoe mice). Five minutes prior to image acquisition, mice were given an intrajugular bolus of Exia 160 (5 μL/g body weight, Binitio Biomedicals, Ontario, Canada). Micro-CT data were acquired with an x-ray source of 50 kVp tube voltage, 48 mA tube current, 11 × 11 detector binning model, 40 ms exposure per projection for contrast-enhanced CT acquisitions. A single frame of 480 projections for 4:58 min of continuous x-ray exposure was used without cardiac or respiratory gating. Volumetric micro-CT images were reconstructed using a Feldkamp algorithm with calibrated Hounsfield units. Image data were transferred to an image processing workstation (Advanced Workstation, version 4.4, GE Healthcare) for further reconstruction and quantitative analysis. The whole aortic tree was segmented and inner luminal diameter of the descending thoracic aorta was calculated.

Chen P.Y., Qin L., Li G., Malagon-Lopez J., Wang Z., Bergaya S., Gujja S., Caulk A.W., Murtada S.I., Zhang X., Zhuang Z.W., Rao D.A., Wang G., Tobiasova Z., Jiang B., Montgomery R.R., Sun L., Sun H., Fisher E.A., Gulcher J.R., Fernandez-Hernando C., Humphrey J.D., Tellides G., Chittenden T.W, & Simons M. (2020). Smooth muscle cell reprogramming in aortic aneurysms. Cell stem cell, 26(4), 542-557.e11.

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In Vivo SPECT/CT Imaging of Xenograft Tumors

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For SPECT/CT imaging, EJ xenograft-bearing mice were anesthetized with 0.8–1.8% isoflurane in air; tumor-bearing mice (n = 3 per group) received intratumoral injections of 15 MBq of either ¹¹¹In-NOTA-DTox-HMP-NLS-EGF or control ¹¹¹In in Hanks solution in a volume equal to half of the tumor volume. Whole body imaging was performed on a U-SPECT-II/CT scanner (MILabs, Utrecht, Netherlands) beginning immediately after injection and continuing for 5 × 10-min frames using a 1.0-mm-diameter pinhole collimator with subsequent immediate whole-animal CT acquisition. For high resolution tumor imaging, a 0.35-mm-diameter pinhole collimator was used. Additional SPECT/CT imaging was performed during the subsequent days (3–5 frames × 10 min). Images were reconstructed using U-SPECT-Rec2.34b software from the manufacturer of the instrument, followed by coregistration of SPECT images to the corresponding CT images. Quantitative analysis of images after 3D-reconstruction was performed using PMOD 3.4 software (PMOD Technologies Ltd., Switzerland).
The radioactivity concentration in tumor and other organs or tissues was determined by selecting a few spheres within the organ or tissue of interest followed by division of the summarized signal within each sphere by its volume.

Rosenkranz A.A., Slastnikova T.A., Karmakova T.A., Vorontsova M.S., Morozova N.B., Petriev V.M., Abrosimov A.S., Khramtsov Y.V., Lupanova T.N., Ulasov A.V., Yakubovskaya R.I., Georgiev G.P, & Sobolev A.S. (2018). Antitumor Activity of Auger Electron Emitter 111In Delivered by Modular Nanotransporter for Treatment of Bladder Cancer With EGFR Overexpression. Frontiers in Pharmacology, 9, 1331.

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Multimodal Imaging of Glioma in Mice

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SPECT/CT images
were obtained using
a u-SPECT-II/CT scanner (Milabs, Utrecht, The Netherlands) equipped
with a 0.6 mm multi-pinhole collimator. The glioma-bearing mouse was
injected with ∼37 MBq of ^99mTc-P6G-RGD₂ in 0.1 mL saline via the tail vein. At 60 min p.i., the animal was
placed into a shielded chamber connected to an isoflurane anesthesia
unit (Univentor, Zejtun, Malta). Anesthesia was induced using an air
flow rate of 350 mL/min and ∼3.0% isoflurane. After induction
of anesthesia, the animal was immediately placed supine on the scanning
bed. The air flow rate was then reduced to ∼250 mL/min with
∼2.0% isoflurane. Rectangular scans in the regions of interest
(ROIs) from SPECT and CT were selected on the basis of orthogonal
optical images provided by the integrated webcams. After SPECT (75
projections over 30 min per frame, 2 frames), the animal was transferred
into the CT scanner and imaged using “normal” acquisition
settings (2° intervals) at 45 kV and 500 μA. After CT acquisition,
the animal was allowed to recover in a lead-shielded cage.

Yang Y., Ji S, & Liu S. (2014). Impact of Multiple Negative Charges on Blood Clearance and Biodistribution Characteristics of 99mTc-Labeled Dimeric Cyclic RGD Peptides. Bioconjugate Chemistry, 25(9), 1720-1729.

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Multimodal Imaging of Tumor-Bearing Mice

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SPECT/CT images were obtained using a u-SPECT-II/CT scanner (Milabs, Utrecht, The Netherlands) equipped with a 1.0 mm multi-pinhole collimator. The tumor-bearing mouse was injected with ~37 MBq of ^99mTc radiotracer (~ 1.0 µg HYNIC-conjugated cyclic RGD peptide) in 0.1 mL saline via the tail vein. At 60 min p.i., the animal was placed into a shielded chamber connected to an isoflurane anesthesia unit (Univentor, Zejtun, Malta). Anesthesia was induced using an air flow rate of 350 mL/min and ~3.0% isoflurane. After induction of anesthesia, the animal was immediately placed supine on the scanning bed. The air flow rate was then reduced to ~250 mL/min with ~2.0% isoflurane. Rectangular scan in the regions of interest (ROIs) from SPECT and CT were selected on the basis of orthogonal optical images provided by the integrated webcams. After SPECT acquisition (75 projections over 30 min per frame, 2 frames), the animal was then transferred into the attached CT scanner and imaged using the ‘normal’ acquisition settings (2 degree intervals) at 45 kV and 500 µA. After CT acquisition, the animal was allowed to recover in a lead-shielded cage.

Zhao Z.Q., Yang Y., Fang W, & Liu S. (2016). Comparison of Biological Properties of 99mTc-Labeled Cyclic RGD Peptide Trimer and Dimer Useful as SPECT Radiotracers for Tumor Imaging. Nuclear medicine and biology, 43(11), 661-669.

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Small Animal SPECT Imaging with Technetium Tracer

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SPECT images of SD rats (n = 3) were obtained using a u-SPECT-II/CT scanner (Milabs, Utrecht, The Netherlands) equipped with a 1.0 mm multi-pinhole collimator. The animal was placed into a shielded chamber connected to an isoflurane anesthesia unit (Univentor, Zejtun, Malta). Anesthesia was induced using an air flow rate of 350 mL/min and ~3.0% isoflurane, and maintained using the air flow rate of ~250 mL/min with ~2.5% isoflurane during the whole time of preparation and image data acquisition (6 frames: 75 projections over 5 min per frame).
The animal was administered with the ^99mTc radiotracer (120 – 180 MBq) in 0.5 mL saline containing ~20% propylene glycol through a catheter, followed with 0.5 mL saline solution flash. Rectangular scan in regions of interest (ROIs) from SPECT and CT were selected one the basis of the orthogonal X-ray images provided by the CT. After SPECT acquisition, the animal was allowed to recover in a shielded cage.

Zheng Y., Ji S., Tomaselli E., Ernest C., Freiji T, & Liu S. (2014). Effect of Co-Ligands on Chemical and Biological Properties of 99mTc(III) Complexes [99mTc(L)(CDO)(CDOH)2BMe] (L = Cl, F, SCN and N3; CDOH2 = Cyclohexanedione Dioxime). Nuclear medicine and biology, 41(10), 813-824.

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SPECT Imaging of 99mTc-ISboroxime-N3 in Rats

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SPECT study was performed the u-SPECT-II/CT scanner (Milabs, Utrecht, The Netherlands) equipped with a 1.0 mm multi-pinhole collimator. The SD rat was placed into a shielded chamber connected to an isoflurane anesthesia unit (Univentor, Zejtun, Malta). Anesthesia was induced using an air flow rate of 350 mL/min and ~3.0% isoflurane, and maintained using an air flow of ~250 mL/min with ~2.5% isoflurane during image data acquisition (6 frames: 75 projections over 5 min per frame). ^99mTc-ISboroxime-N₃ (~185 MBq dissolved in 0.5 mL saline containing ~20% propylene glycol) was injected into the animal via tail vein through a catheter, followed with 0.5 mL saline solution flash. Rectangular scan in regions of interest (ROIs) from SPECT and CT were selected one the basis of orthogonal X-ray images provided by CT. After SPECT acquisition, the animal was allowed to recover in a cage.

Liu M., Zheng Y., Avcibasi U, & Liu S. (2016). Novel 99mTc(III)-azide complexes [99mTc(N3)(CDO)(CDOH)2B-R] (CDOH2 = cyclohexanedione dioxime) as potential radiotracers for heart imaging. Nuclear medicine and biology, 43(11), 732-741.

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