Trimethylsilyldiazomethane solution

Manufactured by Merck Group

Sourced in United Kingdom

Trimethylsilyldiazomethane solution is a laboratory reagent used in organic synthesis. It is a source of the trimethylsilyldiazomethane functional group, which can be used in various chemical reactions. The solution is typically prepared in an organic solvent such as hexane or diethyl ether.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using trimethylsilyldiazomethane solution

Quantifying Auxin Levels in Arabidopsis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Endogenous free IBA and IAA were quantified from shoot and root segments of 9-days-after-germination (dag) light-grown (16 h) Arabidopsis seedlings by using gas chromatography-mass spectrometry (GC–MS) as described in Leljak-Levanic et al. (2010) (link). Methylation was performed by adding equal sample amounts of a 1:10 diluted solution (in diethylether) of trimethylsilyldiazomethane solution (Sigma-Aldrich) for 30 min at room temperature. The mixture was then evaporated and resuspended in 50 μl of ethyl acetate for GC–MS analysis. Data are means of four independent lots of 30–50 seedlings each, and equivalent to ca. 100 mg root shoot material, respectively.
Homozygous generations of Arabidopsis abcg36-4 and abcg36-4 abcg37-2 expressing DR5:GFP were obtained by crossing with DR5:GFP lines (Ottenschlager et al., 2003 (link)). Seedlings were grown vertically for 5 (dag) and then for 4 h on 5 (M IBA plates and analyzed by confocal laser-scanning microscopy. DR5:GFP signals in the very root tip were analyzsed using the Fiji software³.

Aryal B., Huynh J., Schneuwly J., Siffert A., Liu J., Alejandro S., Ludwig-Müller J., Martinoia E, & Geisler M. (2019). ABCG36/PEN3/PDR8 Is an Exporter of the Auxin Precursor, Indole-3-Butyric Acid, and Involved in Auxin-Controlled Development. Frontiers in Plant Science, 10, 899.

+ Open protocol

+ Expand

Synthesis of Methacrylic Acid Diblock Copolymers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methacrylic
acid (MAA), 2-hydroxybutyl methacrylate
(HBMA; actually a 1:1 molar ratio of 2- and 4-isomers as judged by ¹H NMR spectroscopy^{15 (link)}), and 4,4′-azobis(4-cyanovaleric
acid) (ACVA) were purchased from Sigma-Aldrich U.K. and used as received
unless otherwise specified. Deionized water was used in all experiments.
4-cyano-4-(2-phenylethanesulfanylthiocarbonyl)sulfanylpentanoic acid
(PETTC) was prepared as described previously.^{39 (link)} The trimethylsilyldiazomethane solution (2.0 M in diethyl
ether), THF (HPLC, ≥99.9%), and glacial acetic acid (≥99.85%)
used for the preparation and analysis of the methylated diblock copolymers
were also purchased from Sigma-Aldrich U.K. Methanol-d₄, dimethyl sulfoxide-d₆,
and dimethylformamide-d₇ used for ¹H NMR spectroscopy were purchased from Goss Scientific Instruments
Ltd. (Cheshire, U.K.). All other solvents were purchased from Sigma-Aldrich
U.K. or VWR Chemicals.

Cockram A.A., Neal T.J., Derry M.J., Mykhaylyk O.O., Williams N.S., Murray M.W., Emmett S.N, & Armes S.P. (2017). Effect of Monomer Solubility on the Evolution of Copolymer Morphology during Polymerization-Induced Self-Assembly in Aqueous Solution. Macromolecules, 50(3), 796-802.

+ Open protocol

+ Expand

Synthesis of Methacrylate Polymer Precursors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methyl
methacrylate (MMA, 99%), ethyl methacrylate
(EMA, 99%), n-butyl methacrylate (BMA, 99%), n-hexyl methacrylate (HMA, 99%), DMAEMA (99%), and MAA (99.5%)
were purchased from Sigma-Aldrich (Gillingham, U.K.) and were passed
through a basic alumina column to remove inhibitor prior to polymerization.
2-Ethylhexyl methacrylate (EHMA, 98%) was purchased from Alfa Aesar
(Heysham, U.K.), and its inhibitor was removed using an alumina column.
Isopropanol (IPA, 99.9%), triethanolamine (TEA, 99%), 4,4′-azobis(4-cyanovaleric
acid) (ACVA), 1,4-dioxane (99.5%), trimethylsilyldiazomethane solution
(2.0 M in diethyl ether), benzyl bromide (BzBr, 98%), and deuterated
acetone were purchased from Sigma-Aldrich. Deuterated chloroform (CDCl₃) and deuterated dimethyl sulfoxide (DMSO-d₆) for NMR spectroscopy measurements were purchased from
VWR (Lutterworth, U.K.). Deionized water was obtained using an Elgastat
Option 3A water purifier (Elga, High Wycombe, U.K.). 4-Cyano-4-(2-phenylethanesulfanylthiocarbonyl)sulfanylpentanoic
acid (PETTC) used for RAFT polymerization was prepared and purified
in-house, as reported previously.^{35 (link)} Unless
stated otherwise, all materials were used as received.

Neal T.J., Parnell A.J., King S.M., Beattie D.L., Murray M.W., Williams N.S., Emmett S.N., Armes S.P., Spain S.G, & Mykhaylyk O.O. (2021). Control of Particle Size in the Self-Assembly of Amphiphilic Statistical Copolymers. Macromolecules, 54(3), 1425-1440.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!