Rapamycin (Rapa) was purchased from Tocris Bioscience (Bristol, UK). Cycloheximide (CHX), tunicamycin (TUN), 6-diazo-5-oxo-L-norleucine (DON), reduced L-glutathione (GSH), reduced glutathione ethyl ester (GSHee) were from Sigma-Aldrich (St. Louis, MO, USA). OU749, buthionine sulfoximine (BSO), and ferrostatin-1 (Fer-1) were from Cayman Chemicals (Ann Arbor, MI, USA). Antibodies specific for p70S6 kinase, phospho-p70S6 kinase (Thr 389), S6 ribosomal protein, phospho-S6 ribosomal protein (Ser 235/236), eIF2α, phospho-eIF2α (Ser 51), PERK, Bip and horseradish peroxidase (HRP)-linked secondary antibodies were from Cell Signaling Technology (Danvers, MA, USA). Antibody specific for phospho-GCN2 (Thr 899) was from Abcam (Cambridge, UK). ATF4 antibody was from Santa Cruz Biotechnology (Dallas, TX, USA).
Buthionine sulfoximine bso
Manufactured by Cayman Chemical
Sourced in United States
Buthionine sulfoximine (BSO) is a laboratory chemical used as an inhibitor of glutathione synthesis. It functions by inhibiting the enzyme gamma-glutamylcysteine synthetase, which is a key step in the production of glutathione.
Automatically generated - may contain errors
Lab products found in correlation
Reduced glutathione ethyl ester, by Merck Group (1 mentions)
Horseradish peroxidase hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
Ferrostatin 1 fer 1, by Cayman Chemical (1 mentions)
L glutathione reduced, by Merck Group (1 mentions)
Ou749, by Cayman Chemical (1 mentions)
6 diazo 5 oxo l norleucine, by Merck Group (1 mentions)
Phospho s6 ribosomal protein ser235 236, by Cell Signaling Technology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Phospho erk, by Santa Cruz Biotechnology (1 mentions)
Primary antibody against actin, by Merck Group (1 mentions)
Phospho nrf2, by Abcam (1 mentions)
Primary antibodies against nrf2, by Cell Signaling Technology (1 mentions)
Z val ala asp ome ch2f z vad fmk, by Peptide Institute (1 mentions)
Dl propargylglycine ppg, by Merck Group (1 mentions)
3 protocols using buthionine sulfoximine bso
1
Modulation of mTOR and eIF2α Pathways
2
Galangin's Modulation of Cellular Antioxidant Pathways
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Galangin (3,5,7-trihydroxyflavone) and primary antibodies against ERK, phospho-ERK, GCLC, and GSS were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and primary antibody against actin were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Buthioninesulfoximine (BSO) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies against TATA-binding protein (TBP) and phospho-Nrf2 were purchased from Abcam (Cambridge, MA, USA). Primary antibodies against Nrf2, AKT, and phospho-AKT were purchased from Cell Signaling Technology (Beverly, MA, USA). Cell TrackerTM Blue CMAC was purchased from Molecular Probes (Eugene, OR, USA). All other chemicals and reagents were of analytical grade.
3
Pharmacological Modulation of Glutathione Metabolism
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CDDP, SAS, and DL-propargylglycine (PPG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Buthionine sulfoximine (BSO) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Z-Val-Ala-Asp(OMe)-CH2F (Z-VAD-FMK) was purchased from Peptide Institute, Inc. (Osaka, Japan). Anti-xCT antibody (ab37185) was purchased from Abcam (Cambridge, UK). Anti-cleaved poly (ADP-ribosyl) polymerase (PARP) antibody (#9541) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Anti-GCLC antibody (GTX16315) was purchased from Gene Tex, Inc. (San Antonio, TX, USA). Anti-cystathionine gamma lyase (CGL) antibody (12217-1-AP) was purchased from Proteintech (San Antonio, TX, USA).
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