Glc 603

Manufactured by Nu-Chek Prep

Sourced in United States

The GLC-603 is a gas chromatography instrument designed for the analysis and separation of volatile organic compounds. It features a high-performance flame ionization detector (FID) for sensitive and accurate detection of sample components. The GLC-603 is capable of providing quantitative and qualitative analysis of a wide range of organic compounds.

Automatically generated - may contain errors

Lab products found in correlation

Rtx 2330 column, by Restek (1 mentions) Gc 2010, by Shimadzu (1 mentions) Gcms qp2010 plus, by Shimadzu (1 mentions) Glc 463, by Nu-Chek Prep (1 mentions) Hexane, by Vetec (1 mentions) Isopropanol, by Vetec (1 mentions) Sodium sulfate na2so4, by Vetec (1 mentions) Milli q system, by Merck Group (1 mentions) Glc 643, by Nu-Chek Prep (1 mentions) Cpsil 88 column, by Agilent Technologies (1 mentions)

3 protocols using glc 603

Lipid Analysis of A. actinomycetemcomitans

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipid analysis was performed based on the method of Whittaker et al. using cells grown in liquid culture [23 (link)]. Mid-logrithmic phase A. actinomycetemcomitans was grown in 1L broth, collected by centrifugation and washed once with PBS. Fatty acids were saponified, methylated and extracted with methyl tert-butyl ether (MTBE) followed by a second extraction of the organic phase with 0.3N NaOH. Analysis was performed using a gas chromatograph (GC2010, Shimadzu, Kyoto, Japan) equipped with a split injector (temperature: 260°C, injection volume: 1 μL, split ratio: 1:20) and a mass spectrometer (GCMS-QP2010 Plus, Shimadzu, Kyoto, Japan) using a Rtx-2330 column (30 m length × 0.25 mm diameter × 0.20 μm film thickness; Restek Bellefonte, PA). FAME were detected in full scan mode (m/z 45 to 500) and identification was achieved using authentic standard mixtures (BAME mix, Supelco Inc., Bellefonte, PA; GLC-463 and GLC-603 from Nu-Chek Prep. Elysian, MN). Mass spectra were based on the web-accessible mass spectra database at American Oil Chemists’ Society (AOCS) lipid library (http://lipidlibrary.aocs.org/).

Smith K.P., Fields J.G., Voogt R.D., Deng B., Lam Y.W, & Mintz K.P. (2015). Alteration in abundance of specific membrane proteins of Aggregatibacter actinomycetemcomitans is attributed to deletion of the inner membrane protein MorC. Proteomics, 15(11), 1859-1867.

+ Open protocol

+ Expand

Chocolate Samples Fatty Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thirty-two chocolate samples (white, dark, and milk chocolates) from different national and multinational brands produced in Brazil were used to carry out this research. Samples were purchased in local markets. The addition of PHVO is still acceptable in this country, and some products had high trans fats contents declared. All reagents used in the experiments were of analytical grade, and water used in aqueous solutions was purified by deionization (Milli-Q system; Millipore, Bedford, MA, USA). Methanol, potassium hydroxide (KOH) and sodium bisulfate (NaHSO4) were purchased from Panreac Química S.A. (Madrid, Spain). Hexane, isopropanol, and sodium sulfate (Na2SO4) were obtained from Vetec (Rio de Janeiro, RJ, Brazil). Analytical FAME standards (GLC-603, GLC-611, GLC-643, GLC-80, GLC-409) were acquired from Nu-Chek Prep Inc. (Elysian, MN, USA).

Amorim T.L., Duarte L.M., de Oliveira M.A.L., de la Fuente M.A, & Gómez-Cortés P. (2020). Prediction of Fatty Acids in Chocolates with an Emphasis on C18:1 trans Fatty Acid Positional Isomers Using ATR-FTIR Associated with Multivariate Calibration. Journal of agricultural and food chemistry, 68(39).

+ Open protocol

+ Expand

Hamburger Lipid Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hamburger samples were thawed and lipids extracted with 2:1 chloroform:methanol according to Folch et al. [20 (link)]. Separate 1.5 mol/L methanolic HCl and 0.5 mol/L sodium methoxide methylations of hamburger lipid extracts (10 mg) were performed according to Kramer et al. [21 (link)] with the inclusion of 1 mg c10-17:1 (Nu-Chek Prep. Inc. Elysian, MN, USA) as an internal standard. Fatty acid methyl esters were analysed by GC using a CP-Sil88 column (100 m, 25 μm ID, 0.2 μm film thickness, Varian Inc., Walnut Creek, CA, USA) using complementary temperature programs with 150°C and 175°C plateaus according to Kramer et al. [21 (link)]. Conjugated linoleic acid (CLA) isomers not separated by GC were further analysed using Ag⁺-HPLC as described by Cruz-Hernandez et al. [22 (link)]. Individual peaks were identified using reference standards (GLC-603, Nu-Chek Prep. Inc., Elysian, MN, USA; BC-Mix1, Applied Science, State College, PA, USA) and peak order and retention times reported in the literature [21 (link)–23 (link)]. Only groups/families of FA and major FA within groups were reported.

Mapiye C., Aalhus J.L., Vahmani P., Rolland D.C., McAllister T.A., Block H.C., Uttaro B., Proctor S.D, & Dugan M.E. (2014). Improving beef hamburger quality and fatty acid profiles through dietary manipulation and exploitation of fat depot heterogeneity. Journal of Animal Science and Biotechnology, 5(1), 54.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!