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Anti mouse ifn γ

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Anti-mouse IFN-γ is a laboratory reagent used for the detection and quantification of mouse interferon-gamma (IFN-γ) in various biological samples. It is a specific antibody that binds to and recognizes the IFN-γ protein produced by mouse cells.

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Lab products found in correlation

Anti mouse cd11c, by BioXCell (2 mentions) Anti mouse cd8, by BioXCell (2 mentions) Anti mouse cd25, by BioXCell (2 mentions) Armenian hamster igg, by BioXCell (2 mentions) Rat igg1, by BioXCell (2 mentions) Anti mouse il 12 p40, by BioXCell (2 mentions) Clone xmg1, by BioXCell (2 mentions) Pc 61, by BioXCell (1 mentions) Recombinant mouse il 23, by BioLegend (1 mentions) Mog35 55 peptide, by Mimotopes (1 mentions) Clone pc 61, by BioXCell (1 mentions) Anti mouse cd4, by BioXCell (1 mentions) Clone gk1, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions) Aim 5 medium, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Interleukin 4 (il 4), by Miltenyi Biotec (1 mentions) Interleukin 6 (il 6), by Miltenyi Biotec (1 mentions) Tgf β, by Miltenyi Biotec (1 mentions) Il 12, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

Anti-IFN-γ (46 citations) Anti-mouse IFN-γ (XMG1.2 (6 citations) Anti-mouse IFN-γ clone XMG1.2 (5 citations) IFN-γ (3 citations) Anti-mouse IFN-γ Ab(R4–6A2, (2 citations) Purified rat anti-mouse IFN-γ mAb (clone XMG1.2) (2 citations)

7 protocols using anti mouse ifn γ

1

Treg Cell Ablation and Depletion Protocol

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For Treg cell ablation studies, DT (Sigma-Aldrich) was injected
intraperitoneally (i.p.) at 50 µg per kg of body weight at the indicated
times. For neutralization and depletion studies, anti-mouse IFN-γ (1 mg,
clone XMG1.2, Bio X Cell) or anti-mouse CD11c (500 µg, clone N418, Bio X
Cell) or anti-mouse CD8 (250 µg, clone 53–6.72, Bio X Cell) or
anti-mouse CD4 (150 µg, clone GK1.5, Bio X Cell) or anti-mouse CD25 (500
µg, clone PC-61.5.3, Bio X Cell) were injected i.p. at the indicated
times. Isotype control antibodies used were Rat IgG1 or Armenian hamster IgG or
Rat IgG2a from Bio X Cell, respectively.
Jang J.E., Hajdu C.H., Liot C., Miller G., Dustin M.L, & Bar-Sagi D. (2017). Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Reports, 20(3), 558-571.
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2

Quantification of IL-12 and IFN-γ in LLC Tumors

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LLC tumor tissue sections were prepared as previously described (Oh et al., 2017 (link)). Briefly, tumor tissues were fixed in 2% paraformaldehyde overnight, followed by embedding in optimum cutting temperature (O.C.T.; Tissue-Tek) compound, and were cut into 14-μm sections. The sections were blocked with 1.0% BSA in PBST (PBS+ 0.1% Tween 20) and incubated with anti-mouse IL-12 p40 (Bio X Cell) or anti-mouse IFN-γ (Bio X Cell) at room temperature for 1 hr. After wash 3 times with PBST, the sections were stained with goat anti-rat IgG H&L (Alexa Fluor® 568) at room temperature for 1 hr. The sections were next counterstained with DAPI and mounted on glass slides. Images were acquired with a LSM880 microscope with Airyscan and FAST Airyscan High Resolution and 32-channel spectral detectors. Relative fluorescence intensity of IL-12 or IFN-γ was quantified by normalizing integrated fluorescence intensity of IL-12 or IFN-γ to the total cell numbers of macrophages, DCs, or CD8+ T cells per mg of tumor.
Wang Y., Song M., Liu M., Zhang G., Zhang X., Li M.O., Ma X., Zhang J.J, & Huang X.Y. (2021). Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity. Cell reports, 35(1), 108948.
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3

EAE Adoptive Transfer Protocol

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Donor mice were induced with active EAE as described above, but without pertussis toxin. Eleven days after active EAE induction, dLN cells were collected, RBC lysed, and incubated in 10% RPMI containing 10% FBS (Cat#SZ-0501, Hooke Laboratories Inc., MA, USA) with 20 µg/ml MOG35–55 peptide (Mimotopes, Victoria, Australia), 10 µg/ml anti-mouse IFNγ (Cat#BE0055, Bio X Cell, NH, USA), 20 ng/ml recombinant mouse IL-23 (Cat#589004, Biolegend, CA, USA) at 3 million cells/ml for 72 h at 37 °C, 5% CO2, for in vitro reactivation. In vitro reactivated mouse CD4+ T cells were purified as above, and 20 million in vitro reactivated CD4+ T cells were transferred into recipient mice intraperitoneally. Clinical scoring of recipient mice was performed starting 5 days following adoptive/passive transfer of in vitro reactivated CD4+ T cells, every day until the completion of the experiment.
Cho J.J., Xu Z., Parthasarathy U., Drashansky T.T., Helm E.Y., Zuniga A.N., Lorentsen K.J., Mansouri S., Cho J.Y., Edelmann M.J., Duong D.M., Gehring T., Seeholzer T., Krappmann D., Uddin M.N., Califano D., Wang R.L., Jin L., Li H., Lv D., Zhou D., Zhou L, & Avram D. (2019). Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation. Nature Communications, 10, 701.
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4

Treg Cell Ablation and Depletion Protocols

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For Treg cell ablation studies, DT (Sigma-Aldrich) was injected intraperitoneally (i.p.) at 50 μg per kg of body weight at the indicated times. For neutralization and depletion studies, anti-mouse IFN-γ (1 mg, clone XMG1.2, Bio X Cell), anti-mouse CD11c (500 μg, clone N418, Bio X Cell), anti-mouse CD8 (250 μg, clone 53-6.72, Bio X Cell), anti-mouse CD4 (150 μg, clone GK1.5, Bio X Cell), or anti-mouse CD25 (500 μg, clone PC-61.5.3, Bio X Cell) was injected i.p. at the indicated times. Isotype control antibodies used were rat IgG1, Armenian hamster IgG, or rat IgG2a from Bio X Cell, respectively.
Jang J.E., Hajdu C.H., Liot C., Miller G., Dustin M.L, & Bar-Sagi D. (2017). Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Reports, 20(3), 558-571.
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5

T-cell Polarization Cytokine Assay

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After cell transfection and/or treatment, supernatants were replaced by mouse T-cell culture medium or AIM V medium (Gibco) for human cells containing polarization cytokines as follows: anti-mouse IFN-γ (50 μg/mL, clone XMG 1.2; BioXCell) and anti-mouse IL-4 (50 μg/mL, clone 11B11; BioXCell) for mouse TH0 cells; IL-12 (10 ng/mL, Miltenyi Biotec) and anti-mouse IL-4 for mouse TH1 cells; TGF-β (2 ng/mL, Miltenyi Biotec), IL-4 (20 ng/mL, Miltenyi Biotec), and anti-mouse IFN-γ for mouse TH9 cells; IL-6 (20 ng/mL, Miltenyi Biotec), TGF-β, anti-mouse IFN-γ, and anti-mouse IL-4 for mouse TH17, IL-12 (10 ng/mL, R&D System) and anti-human IL-4 (3.5 μg/mL, clone MP4-25D2; BioXCell) for human TH1 cells, TGF-β (5 ng/mL, Miltenyi Biotec), IL-4 (10 ng/mL, R&D System), and anti-human IFN-γ (3.5 μg/mL, clone NIB42; BioLegend) for human TH9 cells. Unless specified otherwise, cells were cultured for 3 days at 37°C under 5% CO2.
Benoit-Lizon I., Jacquin E., Rivera Vargas T., Richard C., Roussey A., Dal Zuffo L., Martin T., Melis A., Vinokurova D., Shahoei S.H., Baeza Garcia A., Pignol C., Giorgiutti S., Carapito R., Boidot R., Végran F., Flavell R.A., Ryffel B., Nelson E.R., Soulas-Sprauel P., Lawrence T, & Apetoh L. (2022). CD4 T cell-intrinsic STING signaling controls the differentiation and effector functions of TH1 and TH9 cells. Journal for Immunotherapy of Cancer, 10(1), e003459.
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6

Quantification of IL-12 and IFN-γ in LLC Tumors

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LLC tumor tissue sections were prepared as previously described (Oh et al., 2017 (link)). Briefly, tumor tissues were fixed in 2% paraformaldehyde overnight, followed by embedding in optimum cutting temperature (O.C.T.; Tissue-Tek) compound, and were cut into 14-μm sections. The sections were blocked with 1.0% BSA in PBST (PBS+ 0.1% Tween 20) and incubated with anti-mouse IL-12 p40 (Bio X Cell) or anti-mouse IFN-γ (Bio X Cell) at room temperature for 1 hr. After wash 3 times with PBST, the sections were stained with goat anti-rat IgG H&L (Alexa Fluor® 568) at room temperature for 1 hr. The sections were next counterstained with DAPI and mounted on glass slides. Images were acquired with a LSM880 microscope with Airyscan and FAST Airyscan High Resolution and 32-channel spectral detectors. Relative fluorescence intensity of IL-12 or IFN-γ was quantified by normalizing integrated fluorescence intensity of IL-12 or IFN-γ to the total cell numbers of macrophages, DCs, or CD8+ T cells per mg of tumor.
Wang Y., Song M., Liu M., Zhang G., Zhang X., Li M.O., Ma X., Zhang J.J, & Huang X.Y. (2021). Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity. Cell reports, 35(1), 108948.
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7

3D Organotypic Tumor Spheroid Assays

Cited 1 time
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Murine-derived and patient-derived organotypic tumor spheroids (MDOTS/PDOTS) were prepared, cultured, and characterized as previously described (summarized in the Supplemental Material).[44 (link)] Immune cells were present from the initial tumor resection, and spheroid-collagen mixtures (10 μL, 2.5 mg/mL tumor spheroids) were injected into the center gel region of the AIM Dax-01 3D microfluidic culture device (AIM Biotech, Singapore). After incubation (30 minutes at 37°C) in sterile humidity chambers, collagen hydrogels containing MDOTS/PDOTS were hydrated with media (Dulbecco's modified Eagle's medium), with or without the indicated treatments. Treatments included the antibodies anti-human IFNγ (10 μg/mL), anti-mouse IFNγ (10 μg/mL) (both from Bio X Cell), anti-human PD-1 (pembrolizumab), and (S)-mepazine. Murine D4M.3A-derived tumor spheroids and MDOTS were cultured ex vivo with MPT-0308 (0.3-10 μM) for 4 days, and murine MC38-derived MDOTS were cultured with MPT-0308 (3 μM ± anti-mouse IFNγ) or anti-mouse IFNγ alone for 6 days. PDOTS established from patients with colorectal cancer (CRC) and melanoma were cultured ex vivo with MPT-0118 or MPT-0308 (3-5 μM [unless otherwise specified] ± anti-PD-1 [pembrolizumab]) for 5 to 7 days; one experiment additionally included each treatment plus or minus anti-human IFNγ.
Di Pilato M., Gao Y., Sun Y., Fu A., Grass C., Seeholzer T., Feederle R., Mazo I., Kazer S.W., Litchfield K., von Andrian U.H., Mempel T.R., Jenkins R.W., Krappmann D, & Keller P. (2023). Translational Studies Using the MALT1 Inhibitor (S)-Mepazine to Induce Treg Fragility and Potentiate Immune Checkpoint Therapy in Cancer. Journal of Immunotherapy and Precision Oncology, 6(2), 61-73.
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