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Nef710

Manufactured by PerkinElmer
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The NEF710 is a high-performance liquid chromatography (HPLC) system designed for precise and reliable separation and analysis of a wide range of chemical compounds. It features a dual-piston pump, a built-in autosampler, and a diode-array detector, providing a comprehensive solution for routine laboratory applications.

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Lab products found in correlation

Pvdf blocking reagent, by Toyobo (2 mentions) Mouse monoclonal anti flag m2, by Merck Group (1 mentions) Nel741, by PerkinElmer (1 mentions) Nel753, by PerkinElmer (1 mentions) Pk 6102, by Vector Laboratories (1 mentions)

3 protocols using nef710

1

Multicolor Immunohistochemistry Staining Protocol

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Primary antibodies included polyclonal rabbit anti-Ac-p53 (Lys379) (Thermo Scientific), monoclonal rat anti-HDAC9 antibody (clone 45a7b5b), monoclonal mouse anti-FLAG M2 (Sigma), polyclonal rabbit anti-acetylated lysine (Millipore 06-933), polyclonal rabbit anti-BCL6 (ab19011), anti-CD45R(B220) (ab64100) and anti-CD3 (ab5690). Secondary antibodies included: biotinylated rabbit anti-rat secondary antibody (Vector Laboratories, PK-6104), biotinylated horse anti-mouse secondary (Vector Laboratories, PK6102) and biotinylated goat anti-rabbit secondary (Vector Laboratories, PK6101). Fluorochromes and chromogens included SA-HRP (Vector Laboratories, PK-6102), FITC fluorophore tyramide (Perkin Elmer, NEL741, NEL753), FITC-HRP (Perkin Elmer, NEF710), Cy3 fluorophore tyramide (Perkin Elmer, NEL741, NEL753), Cy5 fluorophore tyramide (Perkin Elmer, NEL745) and AEC (AbD Serotec, BUF019B).
Gil V.S., Bhagat G., Howell L., Zhang J., Kim C.H., Stengel S., Vega F., Zelent A, & Petrie K. (2016). Deregulated expression of HDAC9 in B cells promotes development of lymphoproliferative disease and lymphoma in mice. Disease Models & Mechanisms, 9(12), 1483-1495.
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2

Detection of GRP-10 Binding via FITC-Labeled Ligand Blotting

Cited 1 time
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Western ligand blotting was conducted according to our previously described methods (Hasegawa et al., 2017 (link); Hasegawa et al., 2020 (link)). Membranes were blocked with the PVDF blocking reagent (Toyobo) for 30 min at room temperature. After washing three times with TBST, the blot was probed with FITC-GRP-10 (Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met) at 0.34 μM for 30 min at room temperature. Blotted membranes were washed three times with TBST and incubated with HRP-conjugated goat polyclonal antibody against FITC (NEF710, PerkinElmer) at a 1:2,000 dilution in TBST for 1 h at room temperature. Detection of the signals was performed in a similar method as Western blot.
Delacrétaz E., Michalopoulos P.G., Ruiz J., Saner H, & Meier B. (1998). Management of hyperlipidaemia after coronary revascularisation: follow up study. BMJ : British Medical Journal, 316(7143), 1499-1500.
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3

Detection of GRP-10 Binding via FITC-Labeled Ligand Blotting

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western ligand blotting was conducted according to our previously described methods (Hasegawa et al., 2017 (link); Hasegawa et al., 2020 (link)). Membranes were blocked with the PVDF blocking reagent (Toyobo) for 30 min at room temperature. After washing three times with TBST, the blot was probed with FITC-GRP-10 (Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met) at 0.34 μM for 30 min at room temperature. Blotted membranes were washed three times with TBST and incubated with HRP-conjugated goat polyclonal antibody against FITC (NEF710, PerkinElmer) at a 1:2,000 dilution in TBST for 1 h at room temperature. Detection of the signals was performed in a similar method as Western blot.
Takanami K., Oti T., Kobayashi Y., Hasegawa K., Ito T., Tsutsui N., Ueda Y., Carstens E., Sakamoto T, & Sakamoto H. (2022). Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans. The Journal of comparative neurology, 530(16), 2804-2819.
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