Anti-LGR5 (ab75850) and anti-phospho-Smad2 (Ser465/467) (ab3849) antibodies were purchased from Abcam, Cambridge, MA, USA. Anti-cleaved PARP (5625), anti-cleaved caspase 9 (9502), anti-Smad2/3 (8685), anti-Smad4 (9515), anti-TGFβ RII (11888) and anti-actin (4970) antibodies were obtained from Cell Signaling Technology, Danvers, MA, USA. Anti-TGFβ RI (sc-398) was from Santa Cruz, Dallas, Texas, USA. Anti-Flag (F1804) and anti-HA (MMS-101P) antibodies were purchased from Sigma and Biolegend respectively. Recombinant human TGFβ1 and RSPO1 were purchased from R&D Systems, Minneapolis, MN, USA and Origene, Rockville, MD, USA. The TGFβ RI kinase inhibitor (SB525334) and the human RSPO1 neutralizing antibody (AF4645), which is antigen affinity-purified and displays less than 1% cross-reactivity with other human RSPOs, were purchased from Sigma and R&D Systems respectively.
Anti actin 4970
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-actin (4970) is a primary antibody that detects actin, a cytoskeletal protein found in eukaryotic cells. It is suitable for use in various applications, such as Western blotting and immunohistochemistry, to visualize and quantify actin levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti actin 4970
1
TGFβ/RSPO1 Signaling Pathway Inhibition
2
Investigating Ion Channel Protein Expression
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All primer sequences with clone plasmid and identification can be found in Supplementary Table S1. All cells were grown in high-glucose Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1% antibiotic-antimitotic solution (penicillin, streptomycin, and amphotericin B). Cells were tested negative for mycoplasma contamination, but were not authenticated. ZEA was purchased from Sigma (St. Louis, MO, USA) and dissolved in methanol (20 mM). Primary antibodies against KCNJ4 (PA5-102023), KCNJ12 (PA5-55485), MITOK (PA5-52767) and PINK1 (PA5-86941) were purchased from thermos Fisher (Waltham, MA USA), anti-actin (4970) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
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