400mr ft nmr instrument

Optical rotations were determined with a JASCO P1020 digital polarimeter. IR spectra were taken on a JASCO FT/IR-4100 spectrometer. The NMR spectra were recorded on a Varian 400MR FT-NMR instrument at 400 MHz for ¹H and 100 MHz for ¹³C, and on a Varian Unity INOVA 500 FT-NMR spectrometer at 500 MHz for ¹H and 125 MHz for ¹³C in CDCl₃. LR- and HR-ESIMS were measured with a Bruker APEX II mass spectrometer. Silica gel 60 (230–400 mesh, Merck, Darmstadt, Germany) and SiliaBond C18 silica gel (40–63 µm, 60 Å, 17% carbon loading, SiliCycle, Québec, QC, Canada) were used for column chromatography. Precoated silica gel plates (Kieselgel 60 F254, Merck, Darmstadt, Germany) and precoated silica gel RP-18 plates (Kieselgel 60 F254S, Merck, Darmstadt, Germany) were used for TLC analysis.

Optical rotations were measured on a Horiba High Sensitivity Polarimeter SEPA-300. Ultraviolet spectra were recorded on a JASCO V-650 spectrophotometer. IR spectra were recorded on a JASCO FT/IR-4100 infrared spectrophotometer. CD spectra were recorded on a JASCO J-815 CD spectrophotometer. NMR spectra were recorded on a Varian 400MR FT-NMR instrument at 400 MHz for ¹H and 100 MHz for ¹³C in CDCl₃. LRMS and HRMS were obtained by ESI on a Bruker APEX II mass spectrometer. Silica gel (Merck, 230–400 mesh) was used for column chromatography. Precoated silica gel plates (Merck, Kieselgel 60 F-254, 0.2 mm) were used for analytical TLC. High-performance liquid chromatography was performed on a Hitachi L-2455 HPLC apparatus with a Supelco C18 column (250 × 21.2 mm, 5 μm).

Optical rotations and IR spectra were recorded on a JASCO P-1020 polarimeter and FR/IR-4100 infrared spectrophotometer (Jasco Corporation, Tokyo, Japan), respectively. NMR spectra were acquired on a Varian Unity Inova 500 FT-NMR (Varian Inc., Palo Alto, CA, USA) at 500 and 125 MHz for ¹H and ¹³C, respectively; or on Varian 400 MR FT-NMR instrument at 400 MHz and 100 MHz for ¹H and ¹³C, respectively. The data of low-resolution electron spray ionization mass spectroscopy (LRESIMS) were measured by Bruker APEX II mass spectrometer. The data of HRESIMS were obtained by Bruker Apex-Qe 9.4T mass spectrometer. Silica gel 60 and reverse-phased silica gel (RP-18; 230–400 mesh) were used for normal-phase and reverse-phased column chromatography. Precoated silica gel plates (Kieselgel 60 F254, 0.2 mm) (Merck, Darmstadt, Germany) were used for analytical TLC. High-performance liquid chromatography was performed on a Hitachi L-2455 HPLC apparatus with a Supelco C18 column (ODS-3, 5 μm, 250 × 20 mm; Sciences Inc., Tokyo, Japan).