All AVR procedures were performed by one of four experienced cardiothoracic surgeons. The technique was similar between surgeons. Access was via standard median sternotomy. Cardiopulmonary bypass was established using aortic and right atrial cannulation with arrest achieved by high dose tepid blood cardioplegia delivered in an antegrade fashion. Four different tissue valve prostheses were used; two porcine (Medtronic Mosaic®, Medtronic Inc. Minneapolis MN; St Jude Medical Epic®, St Jude Medical Inc. Minneapolis MN), two pericardial (St Jude Medical Trifecta®, St Jude Medical Inc. Minneapolis MN; Edwards Perimount Magna®, Edwards Lifesciences, Irvine CA). No patient received a mechanical prosthesis. Intra-operative TEE was used as needed.
Trifecta
Manufactured by Abbott
Sourced in United States
Trifecta is a versatile laboratory equipment that performs three core functions: sample preparation, analysis, and data management. It is designed to streamline various laboratory workflows, providing efficient and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using trifecta
1
Aortic Valve Replacement Techniques
2
Surgical Valve Selection in Transcatheter Aortic Valve Implantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the Translink study, the choice of BHV type was left to the decision of the cardio-surgical team of each center. Patients were implanted with the most frequently implanted BHVs worldwide: two types of surgical porcine valves: Mosaic or Hancock II (Medtronic); 6 different surgical bovine pericardium valves: Perimount Carpentier–Edwards (Edwards Lifesciences), Magna Ease (Edwards Lifesciences), Trifecta (St Jude Medical), Perceval or SOLO bioprostheses (Sorin Biomedica Cardio) and the Mitroflow PRT valve (Sorin Biomedica Cardio); one surgical equine valve: 3F Valve Enable (Medtronic) and two percutaneous pericardium valves (TAVI): CoreValve (porcine pericardium; Medtronic) and SAPIEN valve (bovine pericardium; Edwards Lifesciences). The operating techniques and surgical valve model selection were left to the operating surgeon’s discretion. The distribution of types of implanted biological prostheses in groups B1/B2 is detailed in Fig. 1b .
3
Subcutaneous Implantation of Bioprosthetic Heart Valves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercial BHVs were examined: Sorin SOLO (two samples), St Jude Medical Trifecta (three samples), Carpentier–Edwards and Medtronic 3f (two samples). Each BHV was cut into seventeen 6-mm diameter discs (KRUUSE biopsy punch); 8 discs were pretreated with affinity-purified human anti-Neu5Gc IgG (10 µg ml−1 in PBS) and another 8 discs were treated with control PBS (each disc in 500 µl), incubated for 48 h at 4 °C, then implanted subcutaneously on the back of Cmah−/− mice. Four discs were implanted in each mouse (two discs on each side), two mice per treatment (antibody or control pretreated discs). For implantation, 6–7-week-old mice were anesthetized subcutaneously (1:1:8 of ketamine:xylazine:saline; 10 µl per g of weight); then the skin was sectioned and the pretreated discs gently washed in PBS were implanted subcutaneously using sterile forceps, followed by sewing of the cut skin. During surgery special care was taken to place the discs deep inside the subcutaneous compartment away from the suture. Mice were killed after one month and discs were explanted for further analysis.
4
Randomized Trial of SAVR vs TAVI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients aged ≥70 years with severe AS assessed by the local heart team were included in the trial. A detailed list of inclusion and exclusion criteria have been described previously.15 (link) Enrolled patients were randomized to SAVR using bioprosthetic valves (St Jude Medical Epic®, 29%; Medtronic Mosaic®, 27%; St Jude Medical Trifecta®, 24%; Carpentier-Edwards Perimount®, 10%; and Sorin Mitroflow®, 10%) or TAVI using the first-generation self-expanding Medtronic CoreValve® in all patients. Patients were followed with clinical and echocardiographic visits at 3 and 12 months, and then yearly after the index procedure.
5
Postdilatation Techniques for Transcatheter Valve Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Postdilatation was mainly performed using a noncompliant balloon (True Dilatation balloon or Atlas Gold balloon, C.R. Bard, Murray Hill, NJ, USA) either due to underexpansion of the THV frame or due to optimization of THV frame expansion within a surgical valve known to resist BVF (i.e., Trifecta, St. Jude Medical, Minneapolis, MN, USA). In two procedures, the balloon provided with the Edwards Sapien THV and in one case a NuCLEUS balloon (NuMED Inc., Hopkinton, USA) were used. For hemodynamic analysis, patients with failed valve fracturing attempts were included in the “postdilatation” group.
6
Phospholipid Content and GA Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FREE-treated samples (n ¼ 6) were compared with pericardial samples from either GA-fixed tissue without any further treatment (GA-only [n ¼ 18]) or samples obtained from commercially available valves: Linxtreated tissue (n ¼ 3) from Trifecta valves (St Jude Medical, Saint Paul, Minn), ThermaFix-treated tissue (n ¼ 3) from CE-Perimount Magna Ease valves (Edwards Lifesciences, Irvine, Calif). Trifecta or Perimount valves were rinsed according to their respective instructions for use. After drying and mechanical pulverization of sample tissues, phospholipids were extracted using a chloroform methanol solution similar to that described by Stewart. 14 Phospholipid content was measured using a spectrophotometric quantification after derivatization with ammonium ferrous thiocyanate. Unbound GA was qualitatively assessed by a fuchsine-based staining technique. For each of the 4 groups (GA-only, FREE, Linx, and ThermaFix), 3 samples were stained. Fuchsine stain turns dark blue in the presence of unbound aldehyde functional groups, whereas the lack of unreacted aldehyde groups will result in very little color change.
7
Trifecta Aortic Valve Replacement Outcomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The internal database of Wessex Cardiothoracic Centre at UHS was searched to identify patients who underwent aortic valve replacement with a Trifecta or Trifecta GT (Abbott, Abbott Park, IL, USA) tissue valve at Southampton General Hospital and Spire Hospital Southampton, during the period January 2011–February 2018.
8
Mechanical and Biological Heart Valve Replacements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The surgical procedures were performed using cardiopulmonary bypass, and cardioplegic arrest was achieved using a cold, crystalloid cardioplegic solution. Standard median sternotomy, upper ministernotomy through the fourth intercostal space, or anterior minithoracotomy was used for the surgical approach. The following mechanical prostheses were used: St Jude Regent, St Jude Master (Abbott Laboratories); ATS Open Pivot (Medtronic); On-X Heart Valve (CryoLife Inc); and Carbomedics (LivaNova). The biological prostheses used in the study were Hancock (Medtronic); Epic, Trifecta (Abbott Laboratories); CROWN PRT, SoloSmart; and the sutureless Perceval S valve (LivaNova). The choice of the implant procedure was made according to the current guidelines and the surgeon's discretion as well as with the input of the fully informed patient.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!