Secondary antibodies for western blot

3-Methyladenine (3-MA) was purchased from Selleckchem (Houston, TX). Antibodies to p-Smad3, Smad3, p-extracellular signal–regulated kinases 1/2 (ERK1/2), ERK1/2, p-EGFR, Beclin-1, Notch1, p-TAK1, TAK1 were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to Collagen I (A2), GAPDH, EGFR, TGFβRI, p-NF-κB (p65), NF-κB (p65), TNF-α, Jagged-1, CD68 and CD3 were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-p-Histone H3 antibody was purchased from Abcam (Cambridge, MA). MCP-1, RANTES, TGF-β1 ELISA kits and antibody to lipocalin-2 (Lcn2) were purchased from R&D systems (Minneapolis, MN). Anti-LC3 antibody was purchased from Novus Biologicals (Littleton, CO). Anti-β-Catenin antibody was purchased from BD Biosciences (San Diego, CA). Anti-Wnt1 antibody was purchased from Rockland (Limerick, PA, U.S.A.). Vectastain ABC kit was from Vector Laboratories (Burlingame, CA). Malondialdehyde (MDA) and superoxide dismutase (SOD) biochemical reagent kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies to α-smooth muscle actin (α-SMA ) and β-actin, secondary antibodies for Western blot, and all other chemicals were purchased from Sigma (St. Louis, MO).