Fibroblasts were grown on coverslips, fixed with paraformaldehyde 4% for 20 min, treated for 20 min with Universal Blocking Reagent (Biogenex, San Ramon, CA, USA), and then incubated with a rabbit anti-human TP53INP1 (ab202026, Abcam, Cambridge, UK; 1:250 dilution) overnight at 4 °C. The cells were incubated with a secondary antibody (donkey anti-rabbit DyLight-488; 1:450 dilution) for 1 h at room temperature incubated with DAPI for 10 min at room temperature, and then rinsed twice with PBS and mounted with Mowiol medium. Images were obtained with a microscope (Olympus, FsV1000) using a UPlan-FLN 40 NA 1.3 objective. Images were processed using Fiji V1.53 software.
Uplan fln 40 na 1.3 objective
Manufactured by Olympus
The UPlan-FLN 40 NA 1.3 objective is a high numerical aperture (NA) objective lens designed for fluorescence microscopy applications. It has a magnification of 40x and a numerical aperture of 1.3, providing high-resolution imaging capabilities. The objective is optimized for use with standard fluorescent dyes and labels, making it suitable for a variety of biological and materials science applications.
Automatically generated - may contain errors
2 protocols using uplan fln 40 na 1.3 objective
1
Immunofluorescence Staining of TP53INP1
2
Immunofluorescence Analysis of Fibroblasts and ELC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fibroblasts and ELC (1 × 104 cells/cm2) were grown on coverslips, fixed with methanol/acetone at −20 °C (2 min each), and then washed and maintained in PBS at 4 °C. After blocking with a commercial reagent (Universal Blocking Reagent; Biogenex), the cells were incubated overnight with a mouse antihuman α-SMA (Cat. No. A2547-0.5ML Lot# 073M4761; Sigma, St. Louis, MO, USA; 1:100 dilution) and rabbit antihuman E-cadherin (H-108, sc-7870 Lot# K0315; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:25 dilution) at 4 °C, and then washed twice with PBS-Tween 0.5% and incubated with secondary antibodies against mouse (DyLight-488) and rabbit (DyLight-549; 1:250 dilution), for one hour at room temperature. The coverslips were rinsed twice with PBS and mounted with medium containing DAPI to stain cell nuclei (Ultra Cruz Mounting Medium; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Images were obtained with a laser-scanning microscope (Olympus, FsV1000) integrated with an inverted microscope IX81 with UPlanFLN 40 × NA 1.3 objective (Olympus) using Fluoview software. Images were processed and merged using Fiji software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!