According to the manufacturer’s instructions, TGs from liver and serum were measured using commercial kits (BC0265; Solarbio, Beijing, China). FFAs in serum were determined using a LabAssay NEFA kit (294-63601; Wako, Osaka, Japan). ALT and AST levels in serum were examined with commercial kits (E-BC-K235-M and E-BC-K236-M; Elabscience, Wuhan, China).
E bc k236 m
Manufactured by Elabscience
Sourced in United States
The E-BC-K236-M is a laboratory equipment product from Elabscience. It serves as a core measurement instrument for scientific research and analysis. The device's primary function is to perform precise quantitative measurements of various parameters within laboratory settings.
Automatically generated - may contain errors
4 protocols using e bc k236 m
1
Measurement of Liver and Serum Lipids
2
Histological and Hematological Evaluation of Organ Toxicity
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For histological examination, various organs (heart, liver, spleen, and kidney) were collected 1 day after the last bleeding (day 26) in the repeated injection experiment or the second day after 4 times weekly injection. All organs were fixed overnight in 4% paraformaldehyde (E672002; Sangon Biotech, Shanghai, China) at 4°C, embedded in paraffin, sectioned, and stained with H&E according to routine protocols. For hematological examinations, whole blood was centrifuged (2,600 × g, 10 min), and plasma was collected. Alanine aminotransferase (ALT/GPT) and aspartate aminotransferase (AST/GOT) levels were measured following the protocols of ALT/GPT Colorimetric Assay Kit (Reitman-Frankel method) (E-BC-K235-M; Elabscience, Wuhan, China) and AST/GOT colorimetric assay kit (Reitman-Frankel method) (E-BC-K236-M; Elabscience, Wuhan, China). TNF-α levels were measured using a Mouse TNF-α ELISA Kit (PT512; Beyotime, Shanghai, China). Standards were fitted with a four-parameter logistic (4PL) curve.
3
Liver Biochemical Marker Quantification
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The triglyceride and cholesterol contents in the liver were measured using a triglyceride quantification colorimetric kit (K622-100, Biovision, CA, United States) and a cholesterol quantification colorimetric kit (K603-100, Biovision, CA, United States) according to the manufacturer’s protocols. Serum alanine transaminase (ALT) and aspartate transaminase (AST) were detected using an ALT quantification kit (E-BC-K235-M, Elabscience, Wuhan, China) and an AST quantification kit (E-BC-K236-M, Elabscience, Wuhan, China) according to the manufacturer’s protocols.
4
Serum ALP and AST Colorimetric Assay
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The levels of serum alkaline phosphatase (ALP) and aspartate aminotransferase (AST) were measured via colorimetric assay kits (ALP: E-BC-K091-S and AST: E-BC-K236-M, Elabscience, Houston, TX, USA) according to the manufacturer's guideline.
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