LAD2 cells (3 × 105) were incubated with Spike-RBD protein (5 μg/mL, Genscript, Z03483) in adherent buffer (1 mM CaCl2, 2 mM MgCl2 and 5% BSA, pH 7.4) for 1 h at 4 °C. The cells were then fixed with 4% paraformaldehyde (Sigma–Aldrich) for 30 min at room temperature and stained with anti-His-tag antibodies (Abmart, M30111S). Subsequently, the cells were stained with goat anti-mouse Alexla Fluor 488-conjugated secondary antibodies (Invitrogen, A11001), and were detected with flow cytometry (BD Accuri C6) and analyzed with the FlowJo 7.6.1 software. In some experiments, LAD2 cells were prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 1 h at 37 °C before the incubation with Spike-RBD protein.
Spike rbd protein
Manufactured by GenScript
The Spike-RBD protein is a laboratory-produced protein that corresponds to the receptor-binding domain (RBD) of the SARS-CoV-2 virus spike protein. This protein can be used for various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti his tag antibody, by Abmart (2 mentions)
Goat anti mouse alexla fluor 488 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Af933, by R&D Systems (1 mentions)
Accuri c6, by BD (1 mentions)
Anti ace2 antibody, by R&D Systems (1 mentions)
2 protocols using spike rbd protein
1
Spike-RBD Protein Binding to LAD2 Cells
2
Spike Protein Binding to LAD2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LAD2 cells (3×10 5 ) were incubated with Spike-RBD protein (5 μg/mL, Genscript, Z03483) in adherent buffer (1mM CaCl 2 , 2mM MgCl 2 and 5% BSA, pH 7.4) for 1h at 4 °C. The cells were then fixed with 4% paraformaldehyde (Sigma-Aldrich) for 30 min at room temperature and stained with anti-His-tag antibodies (Abmart, M30111S). Subsequently, the cells were stained with goat anti-mouse Alexla Fluor 488-conjugated secondary antibodies (Invitrogen, A11001), and were detected with flow cytometry. In some experiments, LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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